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Offlineflipside13
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LC life span..
    #6772222 - 04/10/07 08:26 PM (17 years, 9 months ago)

Hey Gang,

How long does LC last if left at incubation temps? I made some up, got good growth in 3-5 days, then nothing for the past 3 weeks. I used honey, and the myc is a bit brownish. Is it likely still viable after 3 weeks at 75-80 degrees?
Flipside13


--------------------
Flipside13
- I intend to live forever. So far, so good.

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InvisibleRoachMan
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Re: LC life span.. [Re: flipside13]
    #6772264 - 04/10/07 08:32 PM (17 years, 9 months ago)

Still viable, and the LC would probably start growing again if you were able to agitate the solution to get it oxygenated again.


--------------------
"The mistake that I make is to try to come awake in a place you're just supposed to get shit faced or baked."

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OfflineCarharttKidd
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Re: LC life span.. [Re: RoachMan]
    #6772537 - 04/10/07 09:18 PM (17 years, 9 months ago)

I have had more success with karo. Only one way to find out if it is still viable... If you keep LC's in a cool clean place, they will go for months.

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Offlineflipside13
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Re: LC life span.. [Re: CarharttKidd]
    #6772576 - 04/10/07 09:24 PM (17 years, 9 months ago)

Awesome. I just sucked up about 5 cc of brown furry stuff and innoc'ed two 500 ml jars with 5 holes. Will see in a few weeks I guess.
Thanks for the quick feedback.
Flipside13


--------------------
Flipside13
- I intend to live forever. So far, so good.

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Offlinedelafonze19
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Re: LC life span.. [Re: flipside13]
    #6772705 - 04/10/07 09:48 PM (17 years, 9 months ago)

So by agitating it oxygen just get suspended in the solution? I am also curious about what the end product should look like. Will the lliquid become fully colonized. The one I have seen look like treads drifting around. The treads seem to collect around the block spores that came from the syringe.

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InvisiblecoAsTal
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Re: LC life span.. [Re: delafonze19]
    #6772769 - 04/10/07 09:58 PM (17 years, 9 months ago)

I've heard about injecting hydrogen peroxide in after growth stalls out to oxygenate the myc. Verified true?


PS-- I know it kills spores-- I'm talking *after* myc. growth...


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List

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Offlinedelafonze19
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Re: LC life span.. [Re: coAsTal]
    #6772777 - 04/10/07 09:59 PM (17 years, 9 months ago)

Interesting. I thought the h2o2 was to work as a preservative and fight off contamination.

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Offlinefungusfun1
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Re: LC life span.. *DELETED* [Re: delafonze19]
    #6773259 - 04/10/07 11:37 PM (17 years, 9 months ago)

Post deleted by fungusfun1

Reason for deletion: not swim format


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Offlinejash420
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Re: LC life span.. [Re: fungusfun1]
    #6773813 - 04/11/07 03:08 AM (17 years, 9 months ago)

sounds good, just let em keep going.. keep us posted.


--------------------
I love poo, any friend of my mushrooms is a friend of mine..

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Offlineflipside13
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Re: LC life span.. [Re: jash420]
    #6776976 - 04/11/07 09:11 PM (17 years, 9 months ago)

So, am I to take from this is that by agitating the LC you are trying to accomplish reoxygenation of the LC so it can grow more? I have also read that it breaks up the myc so it can be sucked up in a syringe.
Comments?
Flipside13


--------------------
Flipside13
- I intend to live forever. So far, so good.

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Offlinedelafonze19
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Re: LC life span.. [Re: fungusfun1]
    #6777030 - 04/11/07 09:26 PM (17 years, 9 months ago)

That is exactlly what the one I have seen looks like too. Do these ever get fully colonized?

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InvisibleBlutjager
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Re: LC life span.. [Re: fungusfun1]
    #6777142 - 04/11/07 10:03 PM (17 years, 9 months ago)

Quote:

fungusfun1 said:
hey guys i was looking at the LCs i innoc'd the other day and they are at varying stages of fuzziness...i did PF classic, cambodian and pan cyans...that is the order of their vigor as well...the PF classic culture has what looks like a bunch of black spores stuck together and there is this white fuzziness connecting them so its kind of like a little cotton wad with black flecks in it...this is what i want right? i swear the PFs got fuzzy like six hours after i innoc'd the cultures, i was really impressed...the other two are showing some fuzz the last couple of days so its looking good...

peace




Anything you see growing in a matter of hrs is not something that you want to be growing

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Offlinefungusfun1
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Re: LC life span.. [Re: Blutjager]
    #6777779 - 04/12/07 01:35 AM (17 years, 9 months ago)

hey blutjager thanks for stopping in...i kind of thought the same thing myself but the growth is white fuzzy stuff so i don't know...the fuzzy stuff was coming off these black specks too and the only black specks i put in the solution was spores...i guess we will see...thankfully the pan cyan culture has started to take off and it looks really good, all these little white dots sitting in suspension i'm just starting to be able to make them out...anyway i know at least one of these cultures will be ok and i think the next ones will be much better as it will be the second time and i will be more copasetic about sterility...

i used about 3ccs out of each of my 3 syringes for these cultures, i think i'm going to make a glovebox and do 3 more cultures with the balance of the syringes...see how they look in comparison...i'm kind of cheating on this first grow of mine and have ordered presterilized bags of spawn and substrate, lol...less things for me to screw up as a noob to the bulk casing thing...

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Offlinejapa2222
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Re: LC life span.. [Re: fungusfun1]
    #6777888 - 04/12/07 02:40 AM (17 years, 9 months ago)

I'm too lazy to quote it but someone said something about H2O2 being a preservitive. I think how H2O2 would reoxygenated the LC would be through it breaking down. 2H2O2--->2H2O+O2. I'd be careful with using H2O2 in too large of quantities though. Way easier to just shake it a little than risk it.

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InvisibleBlutjager
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Re: LC life span.. [Re: japa2222]
    #6777972 - 04/12/07 03:50 AM (17 years, 9 months ago)

Only once did I do a experiment with peroxide in an lc and it never grew anything at all:thumbdown:

Good luck though I'm sure that you will get a good lc going

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Offlinefungusfun1
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Re: LC life span.. [Re: Blutjager]
    #6800916 - 04/17/07 06:09 PM (17 years, 8 months ago)

ok guys the cultures have gotten going pretty good, my buddy's going to let them go for another day or two and then knock up all his spawn and substrate bags...

he has a couple of questions about inoculating with the mycelia though, if u all don't mind...

at present moment he don't have any sterile needles...he does however have three partially used spore syringes, so here's his plan:

1) squirt balance of spore solution in new LC container
2) fill empty syringe with H2O2.
3) squirt H2O2 into established LC
4) shake LC
5) suck up LC solution into empty/used syringe
6) Wipe needle with iso alcohol
7) inoculate bags through resealable silicon sites

Does this sound like the way to go? he was wondering if he could just pour the peroxide into a shot glass or something and draw it out of that in to the syringe? Also he was wondering if peroxide is totally necessary? it seems like it helps a lot...

peace

Edited by fungusfun1 (04/17/07 10:33 PM)

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Offlinedelafonze19
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Re: LC life span.. [Re: fungusfun1]
    #6800998 - 04/17/07 06:30 PM (17 years, 8 months ago)

I did exaclty all seven of your steps just recentlly. 1 out of four got conaminated and the LC still looks good(better then the one I have iwthout h2o2). So I think you are good to go. Oh yeah I discarded the first h2o2o rinse, refilled,emptied all but 1/2cc and injected that into the LC.

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Offlinecookeman
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Re: LC life span.. [Re: fungusfun1]
    #6801126 - 04/17/07 07:03 PM (17 years, 8 months ago)

Boil some water for 10 minutes or so. Suck up a full syringe of the boiling water and squirt it into a seperate container twice. You now have a perfectly fine syringe to work with. No peroxide needed. In fact adding peroxide to LC can harm the LC mix.


--------------------
“Let’s put it this way – to lump psychedelic mushrooms into the same group as methamphetamine is like lumping the Bible into the same group as Mein Kampf.
I mean shit; they’re both books, right?”

Joe Rogan


R.I.P. - "Bones" - One of the greatest people I've ever had the pleasure of getting to know and become friends with.

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Offlinefungusfun1
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Re: LC life span.. [Re: cookeman]
    #6801795 - 04/17/07 09:41 PM (17 years, 8 months ago)

hey guys thanks for responding :stoned:

delafonze 3 out of 4 sounds pretty good bro...

cookeman that's a good suggestion about sterilizing the syringe, my buddy liked it a lot...however he has read that h2o2 is good for the culture since it kills everything off but the mycelia and adds oxygen for the mycelia...at this point tho its understandable if its overkill, and the LC looks good so why would he mess with it...he's going to inoculate substrate with the cambodian culture first since its notorious for being hardy and contam resistant (reason they were bought)...after that will be the pans...:syringe:


EDIT- so my buddy felt like there was no time like the present and did the above steps but left out the peroxide.  we will see how that goes.  three 5-pound bags of hpoo based substrate were inoculated with cambodian myc and he also made a new PF LC with 7ml of spore solution so that should get going quick, lol.  bags will go in incubator overnight with LCs, then take them out as soon as the myc takes hold.

thanks again guys

peace :stoner:

Edited by fungusfun1 (04/18/07 12:29 AM)

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Offlineflipside13
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Re: LC life span..-update [Re: flipside13]
    #6830911 - 04/25/07 07:05 AM (17 years, 8 months ago)

Hey Gang,

I innoc'ed two jars with that brown stuff that had been sitting for 3 weeks and now I have one jar that is colonizing like mad, and another that is showing signs of starting, so....the brown stuff works! :-)


--------------------
Flipside13
- I intend to live forever. So far, so good.

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OfflineSilllo
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Re: LC life span..-update [Re: flipside13]
    #6830913 - 04/25/07 07:09 AM (17 years, 8 months ago)

Quote:

flipside13 said:
Hey Gang,




Good work, Shaggy!


--------------------
A child's rhyme stuck in my head.
It said that life is but a dream.
I've spent so many years in question
to find I've known this all along.

The truth is in between the 1st and 40th drink.

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