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theanarchyway
funknchunkbuds


Registered: 02/28/07
Posts: 70
Last seen: 17 years, 4 months
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Contamination have to start over, got some questions...
#6770146 - 04/10/07 12:56 PM (17 years, 9 months ago) |
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1st grow, All casings were contaminated by green mold the casing mix was not fully sterilized But with a backup jar remaining, trying the pf tek sounds good, to start smaller and work up.
I have a colonized wbs pint jar of z.
1. Is there any way to do a g2g to a pf jar?
2. I was thinking about doing the mycelium inoculation tek, is this a good idea for my situation?
-------------------- Everything I say on here is Fictional.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
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Re: Contamination have to start over, got some questions... [Re: theanarchyway]
#6770319 - 04/10/07 01:48 PM (17 years, 9 months ago) |
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Quote:
All casings were contaminated by green mold the casing mix was not fully sterilized
Which wasn't the cause. Sterilizing casing mix will actually cause it to contaminate faster. I'd imagine your spawn was contaminated, so I'd be leary of using anything from that batch for grain to grain transfer. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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coAsTal
Friend



Registered: 04/04/06
Posts: 2,970
Loc: 8a
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Re: Contamination have to start over, got some questions... [Re: RogerRabbit]
#6770402 - 04/10/07 02:02 PM (17 years, 9 months ago) |
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Sorry bro-- time to revisit tek tech...
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats
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BlehMaestro
Stranger



Registered: 03/02/07
Posts: 754
Last seen: 12 years, 9 months
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Re: Contamination have to start over, got some questions... [Re: theanarchyway]
#6770417 - 04/10/07 02:05 PM (17 years, 9 months ago) |
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Quote:
theanarchyway said: 1st grow, All casings were contaminated by green mold the casing mix was not fully sterilized
Pasteurize casing mix / bulk substrate. Try again, and you'll be back 'on the horse' in no time.
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professor_mudd
Easy-going drunk



Registered: 01/06/07
Posts: 154
Loc: USofA
Last seen: 17 years, 7 months
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Re: Contamination have to start over, got some questions... [Re: BlehMaestro]
#6770549 - 04/10/07 02:29 PM (17 years, 9 months ago) |
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RR said:
Quote:
Sterilizing casing mix will actually cause it to contaminate faster.
Why??
-P.M.-
-------------------- All I have written is and always will be fiction, with nothing anchored in the realm of reality.
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Blirska
Stranger


Registered: 03/15/07
Posts: 52
Last seen: 9 years, 7 months
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Re: Contamination have to start over, got some questions... [Re: professor_mudd]
#6770592 - 04/10/07 02:38 PM (17 years, 9 months ago) |
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Because some bacterias present in the casing mix will slow down foreign contaminants. The point of pasteurasition is to get the population of microorganisms down to a ''safe'' level while not killing it completely.
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xaxphaanes
Mycologist


Registered: 08/08/05
Posts: 2,988
Last seen: 2 years, 5 months
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Re: Contamination have to start over, got some questions... [Re: professor_mudd]
#6770593 - 04/10/07 02:38 PM (17 years, 9 months ago) |
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because when you dont sterilize it, it has bacteria that fights off contamination.if you sterilize it, it will kill the bacteria so nothing is fighting off contamination.at least this is what it states in mc
-------------------- "Anything i say is fictional"
what you should look for in manure
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theanarchyway
funknchunkbuds


Registered: 02/28/07
Posts: 70
Last seen: 17 years, 4 months
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Re: Contamination have to start over, got some questions... [Re: xaxphaanes]
#6770620 - 04/10/07 02:43 PM (17 years, 9 months ago) |
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the jar is not contaminated, solid white. The spawn I used was not contaminated and colinized great. It wasnt until the casing layer that contams took over.
-------------------- Everything I say on here is Fictional.
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theanarchyway
funknchunkbuds


Registered: 02/28/07
Posts: 70
Last seen: 17 years, 4 months
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Re: Contamination have to start over, got some questions... [Re: theanarchyway]
#6770630 - 04/10/07 02:45 PM (17 years, 9 months ago) |
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has anyone had any experience with the mycelium inoculation tech?
-------------------- Everything I say on here is Fictional.
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xaxphaanes
Mycologist


Registered: 08/08/05
Posts: 2,988
Last seen: 2 years, 5 months
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Re: Contamination have to start over, got some questions... [Re: theanarchyway]
#6770660 - 04/10/07 02:52 PM (17 years, 9 months ago) |
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Have a link ? for this tek ?how did you make the casing ? what did you use in it exactly ?
-------------------- "Anything i say is fictional"
what you should look for in manure
Edited by xaxphaanes (04/10/07 02:53 PM)
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