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Offlineeltrkbrd
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Registered: 02/23/07
Posts: 65
Last seen: 6 months, 23 days
Choosing between Stereo Microscopes
    #6755337 - 04/06/07 06:53 PM (17 years, 9 months ago)

Can someone please tell me which of these two Scopes is best for Mycology?

Both scopes offer Three Magnification Levels- 10x, 20x and 40x

However, one scope has just a single WF10x Eyepiece whereas the other scope requires both WF5X & WF10X Eyepieces.

For details see:
http://store.amscope.com/se306-px.html
http://www.microscopestore.com/info.asp?itemid=100049&c=33

Also, does anyone know of a more suitable scope in the $100-$200 price range? I've been checking out Stereo Zoom Microscopes (7x-45x).

This one looks really nice but a bit pricy- http://www.cynmar.com/item_detail.aspx?ItemCode=CSZ08636

-TIA


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OfflineRogerRabbitM
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Re: Choosing between Stereo Microscopes [Re: eltrkbrd]
    #6755410 - 04/06/07 07:21 PM (17 years, 9 months ago)

You won't find a good one in that price range. The cheapies all use halogen lighting which puts out so much heat it will fry your specimen before you're done looking at it. The heat also fogs up petri dishes within seconds. Here's the one I use. It's on a boom stand with a flexible arm so I can use it in front of the flowhood, and it has an LED light ring around the objective for good lighting with no heat.
RR



And a few pictures taken with it; From left to right- a tiny piece of perlite no more than .5mm. A tiny piece of verm no more than .5mm, a piece of each side by side at higher magnification, metabolites on a petri dish(the ring of dots is a reflection of the LED's, and last is a cap with the stem cut off, of P nameko.


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OfflineCryogenicz
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Re: Choosing between Stereo Microscopes [Re: eltrkbrd]
    #6755413 - 04/06/07 07:22 PM (17 years, 9 months ago)

It really depends on what you wanna do with your scope.


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InvisibleBlutjager
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Re: Choosing between Stereo Microscopes [Re: Cryogenicz]
    #6756061 - 04/06/07 10:46 PM (17 years, 9 months ago)

So RR how much does a nice specimen like your go for

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Offlineeltrkbrd
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Registered: 02/23/07
Posts: 65
Last seen: 6 months, 23 days
Re: Choosing between Stereo Microscopes [Re: eltrkbrd]
    #6756682 - 04/07/07 02:46 AM (17 years, 9 months ago)

Hi RR,

Where did you get that Variable LED ring light as seen around the objective on your SZ300 Stereo Zoom Microscope in this post? The ring you use looks different than the one listed here: http://www.microscopesusa.com/LED_ring_light.html

Thanks for the warning on the Halogen lighting! Do you still recommend the LW Achiever as a low cost alternative? What do you think about the SZ4R Series in comparison?

Please see:
http://www.microscopesusa.com/Achiever.html
vs.
http://www.microscopesusa.com/stereozoom_4.html

Do you think the SZ4R with Fluorescent Ring lighting would be an effective low cost alternative?
(http://www.microscopesusa.com/fluorescent_ring_light.html)

RR, I remember you mentioned your scope has a 2" diameter objective lense that lets in a lot of light. How did you come up with this figure?

Regarding Eyepieces and Objectives, it seems like a .5x auxillary objective Lens will give a wider field of view than a 1x standard objective although magnification is reduced. Which is better?

I have noticed that for Stereo Microscopes they usually don't list the N.A. for the Obective lens but rather usually only list the field of view presumably for the eyepiece. Which should I be more concerned with?

I looked the following up in a glossary in regards:
Numerical Aperture(N.A.)specifies the diameter of the objective lens.
FN indicates the diameter of the baffle, which determines the field of view of the eyepiece.

For example the SZ300 specs say WFH10x~D/21. I believe this means the Eyepiece is 10x Wide Field with a Diameter of 21mm, the H standing for High Eyepoint meaning for use with glasses? Is all of this correct?

Do we care more about the diameter of the Eyepiece or of the 1.0x Standard Objective Lens?

To be honest I find all of this a bit confusing.

Thanks again!


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My way of giving back- Thanks Shroomery...
A For Newbs by Newbs Cultivation Resource Journal: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6748262/an/0/page/0

Edited by eltrkbrd (04/07/07 06:33 AM)

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Offlineeltrkbrd
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Registered: 02/23/07
Posts: 65
Last seen: 6 months, 23 days
Re: Choosing between Stereo Microscopes [Re: eltrkbrd]
    #6770103 - 04/10/07 12:39 PM (17 years, 9 months ago)

Anyone?


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A For Newbs by Newbs Cultivation Resource Journal: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6748262/an/0/page/0

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OfflineRogerRabbitM
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Re: Choosing between Stereo Microscopes [Re: eltrkbrd]
    #6770311 - 04/10/07 01:45 PM (17 years, 9 months ago)

You want large objective and eyepieces. Both are important. I estimated a 2" objective by putting a tape measure across it. That's pretty close. If you want to be able to see the cystidia on gills, you better not get the .5 objective.

Scroll through my gallery, and you'll see some closeup pictures of mushroom gills that were taken with minimum magnification of .6 for a total of 6X. At the maximum, you can just start to see the basidia, but you'll need the LED ring or better yet, the fiber optic light because very little light makes it through the objective at maximum zoom.

Yes, mine is the SZ300 with flexible boom stand, with the LED light ring shown, or perhaps the later version of it since the color is different. The package cost about $1400, but I already had the CCD tube for my camera. The CCD tube and camera cost about double the cost of the scope, but I can move the tube and camera from scope to scope if I need to use my light microscope. I use the 3 megapixel CCD camera from that site as well to take pictures, but bear in mind, they had to be reduced to upload here. At home, the micrographs are larger than desktop size and quite detailed.

Perhaps the best bet is to give Jeff a call on the phone at the number listed. He'll answer your questions about the features better than I can, and can suggest lower cost alternatives if you don't need to be taking high resolution pictures. I also have one of those eyepiece cameras he sells on the front page of that site. Resolution is much lower, but it does take color pictures. Just don't get talked into halogen lighting, because it's pretty useless for mycology, as said earlier.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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