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Offlinejaranth
Singularitarian
Male


Folding@home Statistics
Registered: 01/25/06
Posts: 80
Loc: Home is where I put my ha...
Last seen: 17 years, 9 months
This happen to you?
    #6766047 - 04/09/07 03:18 PM (17 years, 9 months ago)

THE SETUP:

6 jars were prepared with filter disks. 

Medium used was WBS, prepared/sterilized in the usual way.

All were inoculated with 1.5 cc of B+ spore water from a well-shaken, single syringe under a professional-rated flowhood.

Germination occurred in 5 of the 6 jars.

Two weeks later, myc is growing nicely in 5 jars... however one jar still has nothing.

I don't understand... how could this happen? :confused:


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Offlineatomic1
enthusiast
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Registered: 09/18/03
Posts: 1,123
Loc: Appalachia
Last seen: 5 years, 3 months
Re: This happen to you? [Re: jaranth]
    #6766180 - 04/09/07 03:43 PM (17 years, 9 months ago)

Possible contam, possible that you used the asshole of a spore syringe and didn't get any/enough spores in there to germinate. Hard to say, it may even be a late bloomer. Shake those syringes.

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OfflineCheesekiller
Mad Scientist
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Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 1 year, 20 days
Re: This happen to you? [Re: atomic1]
    #6766242 - 04/09/07 03:55 PM (17 years, 9 months ago)

Try shaking that jar too!


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Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup

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Offlinecoda
Banjo Goiter
Male


Registered: 03/20/01
Posts: 8,750
Last seen: 1 year, 9 months
Trusted Cultivator
Re: This happen to you? [Re: Cheesekiller]
    #6766454 - 04/09/07 04:42 PM (17 years, 9 months ago)

try and waft the jars smell to you, it's always a bad idea to stick your nose right over something that's possibly contaminated, but if you have to be as careful as possible. If it doesn't smell like grain does after it comes out of the PC, if it smells sour and nasty, you have a bacterial contam.


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To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.

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Offlinejaranth
Singularitarian
Male


Folding@home Statistics
Registered: 01/25/06
Posts: 80
Loc: Home is where I put my ha...
Last seen: 17 years, 9 months
Re: This happen to you? [Re: coda]
    #6770859 - 04/10/07 03:35 PM (17 years, 9 months ago)

Hmmmm... right now it's a wait-n-see if the stubborn jar yields anything interesting at all.


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Offlineexagram
Phear the shroom
 User Gallery


Registered: 02/28/07
Posts: 302
Last seen: 16 years, 7 months
Re: This happen to you? [Re: jaranth]
    #6770891 - 04/10/07 03:41 PM (17 years, 9 months ago)

Yup, happened to me. Out of 8 PF jars, 3 came up with nothing. When I re-inoculated them, two started up, one didn't. I tossed that one, even though I didn't smell anything suspicious (just got rid of the substrate, put in some new stuff and inoculated again, things are going fine).

Just put it down to a witch's curse and start over. :tongue:


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Raoul Duke: What Leary took down with him was the central illusion of a whole lifestyle that he helped create - a generation of permanent cripples, failed seekers, who never understood the essential old-mystic fallacy of the acid culture: the desperate assumption that somebody, or at least some force, is tending the light at the end of the tunnel.

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InvisibleSwan Song

Registered: 02/08/06
Posts: 559
Re: This happen to you? [Re: jaranth]
    #6770895 - 04/10/07 03:42 PM (17 years, 9 months ago)

I have had some colonize the inside portion of the grains then finally colonize the outer visable portions.
but I was patient and kept the jar isolated from the rest while waiting.

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