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InvisibleInfested
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Any point in isolating genotype?
    #6755302 - 04/06/07 06:36 PM (16 years, 9 months ago)

Would it be a noticeable difference from using mixed spores, or an isolated mycelium genotype.

What should i expect if i were to plant them separately. And if genotypes can very i would also know how.

If anyone knows anything about this i would appreciate if you tell. Thank you.


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OfflineRogerRabbitM
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Re: Any point in isolating genotype? [Re: Infested]
    #6755325 - 04/06/07 06:47 PM (16 years, 9 months ago)

Isn't this what happens when you use multispore inoculation? Are you talking about mixing spores from different prints of the same species? If so, it can't hurt and could help by bringing in some genetic diversity.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleInfested
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Re: Any point in isolating genotype? [Re: RogerRabbit]
    #6756718 - 04/07/07 03:04 AM (16 years, 9 months ago)

No all same species even the same spore print.

Yes multi spore inoculation and one genotype, isolated from mycelium stage.


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OfflineRogerRabbitM
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Re: Any point in isolating genotype? [Re: Infested]
    #6757670 - 04/07/07 12:26 PM (16 years, 9 months ago)

I have no idea what you're trying to ask.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Invisibleshroomydan
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Re: Any point in isolating genotype? [Re: RogerRabbit]
    #6757978 - 04/07/07 02:07 PM (16 years, 9 months ago)

Cloning vs Multispore.

There is more genetic diversity in multispore, so you should see greater variation in morphology and growth pattern.

I believe the advantage to using a clone is found primarily in vigorous even growth.


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InvisibleSwan Song

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Re: Any point in isolating genotype? [Re: shroomydan]
    #6758486 - 04/07/07 04:32 PM (16 years, 9 months ago)

agreed,
I have done some reading on this and have found that the major advantage is the unifority of colonization and fruit shape and size. Now I am not saying that Isolation makes fruit bigger, it just makes them uniform in size. Other variables contribute to size as we all know; namely, substrate content, substrate depth, and environmental parameters.
Ofcouse, I am no expert and have only read these things so I can't speak on experience.


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OfflineFriendlyNeighbor
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Re: Any point in isolating genotype? [Re: Swan Song]
    #6758741 - 04/07/07 06:20 PM (16 years, 9 months ago)

Once you seperated the desired clone, theoretically, that mycelium culture can be transferred G2G indefinitely, right?

Regards,
FriendlyNeighbor :cool:


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Invisibleagar
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Re: Any point in isolating genotype? [Re: FriendlyNeighbor]
    #6759927 - 04/08/07 01:34 AM (16 years, 9 months ago)

No, it should not be G2G'ed more than 3 times past the mother jar.

After that, the further out you go.
Chances of genetic defects increase.
Plus, as mycelium ages, it loses vigor.

Young vigorous mycelium = young Olympic athlete.
Old mycelium = elderly feeble fart, in rest home.


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InvisibleInfested
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Re: Any point in isolating genotype? [Re: agar]
    #6760308 - 04/08/07 06:50 AM (16 years, 9 months ago)

Tanks guys thats exactly what i wanted to know.

But even grow usually ends up to have higher total production then mixed, correct? After all why would you do it if it didn't.

Also does this mean there will be less/no aborts? If yes does this mean you can chose a bad piece of mycelium and it will only produce aborts :confused:

Thanks again, peace.


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InvisibleSwan Song

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Re: Any point in isolating genotype? [Re: Infested]
    #6803449 - 04/18/07 08:43 AM (16 years, 9 months ago)

I believe that isolate geo's do produce less aborts. (again uniformity) but the type of isolate you have will determine that. IE fuzzy may put off more aborts than rhizo. some experimenting may be necessary.


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OfflineFriendlyNeighbor
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Re: Any point in isolating genotype? [Re: Swan Song]
    #6804007 - 04/18/07 11:30 AM (16 years, 9 months ago)

Assuming that people want the biggest baddest flush, which mushy should they clone?

The biggest fruit from the first, second, or later flushes?

Regards,
FriendlyNeighbor :cool:


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Offlinefiggusfiddus
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Re: Any point in isolating genotype? [Re: FriendlyNeighbor]
    #6806353 - 04/18/07 09:17 PM (16 years, 9 months ago)

Uh. Answering that would require a lot of speculation, guesswork, hobbyist superstition, etc. To be realistic, the mushy you harvest off the left side of a cake or tray in the first flush has a reasonable chance of being the same, genetically, as the one you harvest off the same area in the fourth flush. Just take a nice, big, healthy looking one, gank out the inside of its stem and pop it in the food proceessor (sterile, preferably). Blend well, and serve (to your jars, bags, whatever). Easy peasy, don't overthink it. Your biggest mushroom might not even be genetically predisposed toward size--sometimes that square inch of substrate just happens to be perfectly moist, perfectly rich, whatever. Then again, I'd say the odds are generally in favor of larger fruits if you take tissue from larger samples.


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OfflineFriendlyNeighbor
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Re: Any point in isolating genotype? [Re: figgusfiddus]
    #6806377 - 04/18/07 09:21 PM (16 years, 9 months ago)

Thats a pretty creative cloning method you got there figgusfiddus.

Regards,
FriendlyNeighbor :cool:


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Offlinefiggusfiddus
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Re: Any point in isolating genotype? [Re: FriendlyNeighbor]
    #6806401 - 04/18/07 09:26 PM (16 years, 9 months ago)

Creative, lazy, who knows--I wouldn't trust it to be perfectly sterile, but it's made me upwards of twenty PF-style B+ jars, which in turn have been spawned to bulk. I have yet to see a contaminant problem (not even one jar or myco bag gone bad! 'Cept for a few experimental substrate jars, but those were doomed either way).

Sorry though, didn't mean to derail the thread, just thought I'd toss in my two cents re the likelihood of two fruits from the same patch of mycelium and different flushes being genetically identical.


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InvisiblePsychoslut
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Re: Any point in isolating genotype? [Re: figgusfiddus]
    #6806465 - 04/18/07 09:39 PM (16 years, 9 months ago)

What I would do is clone the biggest mushroom within the biggest cluster. This way there is a good chance the strain you are cloning has a disposition for being a good strong fruiter with good size.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlinefiggusfiddus
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Re: Any point in isolating genotype? [Re: Psychoslut]
    #6806504 - 04/18/07 09:49 PM (16 years, 9 months ago)

That's smart, but more or less equally susceptible to local environmental advantage.


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OfflineFriendlyNeighbor
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Re: Any point in isolating genotype? [Re: figgusfiddus]
    #6807051 - 04/19/07 12:07 AM (16 years, 9 months ago)

True, gotta keep everything as controlled and constant as possible right?

But nonetheless, picking the biggest baddest fruit from a huge cluster is a better strategy than nothing, right?

Regards,
FriendlyNeighbor :cool:


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