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farva86
I dont want alarge farva. Iwant aleeder-a-cola


Registered: 02/23/07
Posts: 267
Loc: MIddle of Nowhere, AR
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salvage contamed LC????
#6752904 - 04/06/07 12:19 AM (16 years, 10 months ago) |
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this is bullshit. this was my second attempt at an lc. first one turned murky...contams...this one is turning murky...fucking contams. i was wondering if there is any possible way to salvage it seeing as how i used the last of my spores on it. maybe a few drops of H2O2??? is it even possible to salvage one from contams???
-------------------- Eddie Money doesn't have to put up with this shit. The wonder has a name: shrooms.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 22 days
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Re: salvage contamed LC???? [Re: farva86]
#6752927 - 04/06/07 12:38 AM (16 years, 10 months ago) |
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I've been saying ever since it started that this silly lc from spores fad is BS. It only sets people back. Spores should be proved on the flat plane of a petri dish before being placed into liquid culture for commercial level production. For the home grower, you're miles ahead inoculating cakes or grains with spores rather than lc which grows at less than half the speed of grains. You can do g2g with healthy grain jars in half the time you can get an lc ready, and you can tell from the smell if a grain jar is good or not, so there's none of the guessing.
Save lc for the commercial guys that have already proved their strains on agar, and use conventional methods if you just want a few mushrooms for home use. You'll be far ahead.
I'm sure there will be a few come in and say 'lc is great' but it's a crapshoot, and luck plays a major part in it. Learn agar skills and you can easily isolate healthy mycelium away from contaminants. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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shroomsynergy
Fungi Freak


Registered: 01/17/06
Posts: 861
Loc: Andromeda
Last seen: 4 years, 5 months
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Re: salvage contamed LC???? [Re: RogerRabbit]
#6752979 - 04/06/07 01:27 AM (16 years, 10 months ago) |
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the majority of prints you'll probably get are infact contaminated. Placing them on a nutrient agar, then cleaning them before creating a LC is almost a requirement today. RR is right, but that is not to say that it cant be done. it's just alot more difficult with all of the bullshit proceedures going around here.
RR is a guru. heed his advice.
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agar
old hand



Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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RR is right (of course). But, many who are taking their first baby steps into mush/cult. Have not yet built a glove box.
Building a laminar flowhood, isn't something they would even consider. And, they are intimidated by agar/perti work. Not to mention, don't have the supplies to do it. Some are also inhibited by budget, others by space, etc.
Spore syringes from long standing Shroomery vendors.
http://sporeworks.com/store/catalog/Psilocybin-Genera-Spores-Major-and-Minor-p-1-c-250.html
http://www.ralphstersspores.com/
Are usually 97% clean/viable.
Otherwise, they would not still be standing. So, odds are in your favor, you can get by with it. (I wish I had 90+% favorable odds, at a crap table.)
As RR has mentioned before: Another way to create LC, if you have the right type LIDS . Is to only fill a wbs/grain spawn jar. A 1/4, 1/3rd or half full of seed/grain. PC the jar as usual & cool to room temps.
Inoculate with spore solution & incubate it. If it grows out healthy & uncontaminated. (you then KNOW you have a clean culture)
Inject 250 ml of sterile water in it. Shake it, then aspirate out X amount of the solution. Which will carry significant amounts of mycelium, in it. (however many syringes you want)
Then, if you want more LC. Use 1 of those syringes to propagate more LC. In a nutrient solution jar, with optimal lids .
BTW, trying to salvage a contaminated LC. IS NOT GOING TO HAPPEN. EXAMPLE: If someone pissed in your chicken noodle soup. Could you salvage it? Answer: --->NO
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: salvage contamed LC???? [Re: agar]
#6753346 - 04/06/07 07:46 AM (16 years, 10 months ago) |
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I agree with RR and his "Liquid mycelium tek" from grain jars is absolutely wonderful,you know its good because you can smell it,I also agree with Agar that providing you got clean syringes and follow basic sterile tek (Glovebox,bleach,etc...)you should be fine in making lc.
I may be a bit biased because long before I could get a jar to be any color but green using multispore,I already had tons of healthy lc.How is this possible,I'm not sure but I am fairly positive it was from other screw ups along the way in prepping my jars (Maybe I had the wrong lids right Agar )
All in all I see the draw in making lc from nutrient solution but it does not really speed things up the way ppl think it does,I am one of those people who has not gotten into working with agar and use still air over a flowhood,I think it is just a matter of preference and when it comes down to it people are going to do what they are comfortable with any way
By the way every time I have shot mycelium from RR's tek into honey water if gets so thick that nothing whatsoever will break it up,I use 2 sharp shards of broken glass in each bottle of lc I make and it is defenseless against this "Super mycelium"
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fetalscab



Registered: 12/28/06
Posts: 463
Loc: not quite sure?
Last seen: 8 years, 8 months
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Re: salvage contamed LC???? [Re: Blutjager]
#6754100 - 04/06/07 12:47 PM (16 years, 10 months ago) |
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see im poor as hell without a bank card and i cant aford agar or find petrie dishes. i think lc is a great way to clone without agar, i dont see any point in making lc from spores but, i use it for cloning and i dont think i will ever do it any other way(until i have a job), its just to fucking easy.
-------------------- The mind is a bright blue sky Clouds are thoughts and feelings floating by You have them but you are not them Whatever you do Don't let them have you
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farva86
I dont want alarge farva. Iwant aleeder-a-cola


Registered: 02/23/07
Posts: 267
Loc: MIddle of Nowhere, AR
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Re: salvage contamed LC???? [Re: fetalscab]
#6754272 - 04/06/07 01:31 PM (16 years, 10 months ago) |
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well im not doing it to speed things up as has been mentioned. i just wanted to use the last of my spores to get some extra myc for more jars. i got my spores from hawks eye and the jars done with that syringe are growing great right now, so i know its not the spores. i do all my work in a still air glove box. obviously my sterile technique needs some work, but i thought i was being pretty damn sterile...guess not. i have 1 jar thats lagging behind and i think ill do a G2G with some of it for RR's liquid myc tek. i dont think ill be doing agar work until i get my own place/get rid of my roomies.
i just thought of this while typing: would it be possible to take a few colonized grains and toss then in an LC? i doubt ill try it just wondering if its feasible.
-------------------- Eddie Money doesn't have to put up with this shit. The wonder has a name: shrooms.
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