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Offlineeltrkbrd
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Registered: 02/23/07
Posts: 65
Last seen: 6 months, 23 days
Agar Flask - Autoclavable Cap vs. Polyfill covered with Tinfoil
    #6754062 - 04/06/07 12:30 PM (17 years, 9 months ago)

Hi all,

I have a 1000ml Kimax Erlenmeyer Flask with Autoclavable Screw Cap for working with Agar and it comes with an inert white rubber liner that is resistant to steam sterilization.

My question is should I use this autoclavable cap by putting it on loosely and then tightening down to seal after the PC cools enough or should I use polyfill stuffed in the top of the flask covered with tinfoil instead?

Also, about how long do I have to let the Petri's cool in the Glovebox before transfering live mycelium to the dishes and wrapping them with Parafilm? (I am covering the Petri's with the plastic slip they came in beforehand.)

I am following RR's recommendations on Pouring Agar and Stacking Dishes in a Glovebox to avoid contamination and condensation on the Petri lids.

-TIA

Edited by eltrkbrd (04/06/07 06:16 PM)

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