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FGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 10 years, 8 months
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5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow
#6751851 - 04/05/07 07:04 PM (17 years, 9 months ago) |
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I inoculated some spawn bags (5 liters of substrate for bag)4 days ago with 40ml of L.C (pan Vietnam) each . When should I see some visible grow?
the substrate is:
12 parts of homemade compost (horse manure/straw based) 8 parts Rye seeds 1 part of verm
this was sterilized for two and a half hours at 15-18psi.
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figment
Apothecary



Registered: 01/04/06
Posts: 522
Loc: Quidity
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: FGL]
#6751866 - 04/05/07 07:07 PM (17 years, 9 months ago) |
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okay, you didn't inject the bag with the spores did you? if you did it's not going to work.
also, i have some pans going now and it seems to me that they take considerably longer to colonize than regular cubes.
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FGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 10 years, 8 months
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: figment]
#6751884 - 04/05/07 07:11 PM (17 years, 9 months ago) |
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Quote:
figment said: okay, you didn't inject the bag with the spores did you? if you did it's not going to work.
also, i have some pans going now and it seems to me that they take considerably longer to colonize than regular cubes.
I used 40ml of liquid culture per bag...
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figment
Apothecary



Registered: 01/04/06
Posts: 522
Loc: Quidity
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: FGL]
#6752046 - 04/05/07 07:59 PM (17 years, 9 months ago) |
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oh okay. you're cool then. pans just take time.
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coAsTal
Friend



Registered: 04/04/06
Posts: 2,970
Loc: 8a
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: figment]
#6752187 - 04/05/07 08:37 PM (17 years, 9 months ago) |
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Are you keeping temps in the low 80's? Perhaps it's a bit too cool...
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats
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agar
old hand



Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: FGL]
#6752405 - 04/05/07 09:25 PM (17 years, 9 months ago) |
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Leave the bags alone. Don't touch/handle them. About 72/74 F is max incubation temperature. (once they start to colonize, they will generate heat).
IGNORE THEM for a few more days.
Pan myc is wispy & looks almost like cobweb. It doesn't look like P. Cubensis myc. The first bag I ever did, I thought was contaminated. It wasn't. Just thin wispy myc colonizing.:p
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figment
Apothecary



Registered: 01/04/06
Posts: 522
Loc: Quidity
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: agar]
#6752637 - 04/05/07 10:37 PM (17 years, 9 months ago) |
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TRUE THAT. it doesn't look like cube myc at all. it's very outstretching and whispy as mentioned above. i think that's why it takes so long to colonize to 100%...b/c it's so thin. patience for real...i inoc'd 10 jars about 3 weeks ago and some are 90%, but there are others that are about 60%...i don't know if it was the syringe,or the conditions. the puerto ricans did great...they were done exactly the same, at the same time. who knows.
but i'm betting on the fact that pans are different. obviously.
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FGL
Stranger

Registered: 06/25/05
Posts: 572
Last seen: 10 years, 8 months
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: figment]
#6753823 - 04/06/07 11:15 AM (17 years, 9 months ago) |
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Many thanks friends! I wont to look at them for a few days 
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