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OfflineFGL
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Registered: 06/25/05
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5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow
    #6751851 - 04/05/07 07:04 PM (17 years, 9 months ago)

I inoculated some spawn bags (5 liters of substrate for bag)4 days ago with 40ml of L.C (pan Vietnam) each . When should I see some visible grow?

the substrate is:

12 parts of homemade compost (horse manure/straw based)
8 parts Rye seeds
1 part of verm

this was sterilized for two and a half hours at 15-18psi.

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Invisiblefigment
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: FGL]
    #6751866 - 04/05/07 07:07 PM (17 years, 9 months ago)

okay, you didn't inject the bag with the spores did you? if you did it's not going to work.

also, i have some pans going now and it seems to me that they take considerably longer to colonize than regular cubes.


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OfflineFGL
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: figment]
    #6751884 - 04/05/07 07:11 PM (17 years, 9 months ago)

Quote:

figment said:
okay, you didn't inject the bag with the spores did you? if you did it's not going to work.

also, i have some pans going now and it seems to me that they take considerably longer to colonize than regular cubes.




I used 40ml of liquid culture per bag...

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Invisiblefigment
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: FGL]
    #6752046 - 04/05/07 07:59 PM (17 years, 9 months ago)

oh okay. you're cool then. pans just take time.


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InvisiblecoAsTal
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: figment]
    #6752187 - 04/05/07 08:37 PM (17 years, 9 months ago)

Are you keeping temps in the low 80's? Perhaps it's a bit too cool...


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Invisibleagar
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: FGL]
    #6752405 - 04/05/07 09:25 PM (17 years, 9 months ago)

Leave the bags alone.
Don't touch/handle them.
About 72/74 F is max incubation temperature.
(once they start to colonize, they will generate heat).

IGNORE THEM for a few more days.:popcorn:

Pan myc is wispy & looks almost like cobweb.
It doesn't look like P. Cubensis myc.
The first bag I ever did, I thought was contaminated.
It wasn't. Just thin wispy myc colonizing.:p


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Invisiblefigment
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Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: agar]
    #6752637 - 04/05/07 10:37 PM (17 years, 9 months ago)

TRUE THAT. it doesn't look like cube myc at all. it's very outstretching and whispy as mentioned above. i think that's why it takes so long to colonize to 100%...b/c it's so thin. patience for real...i inoc'd 10 jars about 3 weeks ago and some are 90%, but there are others that are about 60%...i don't know if it was the syringe,or the conditions. the puerto ricans did great...they were done exactly the same, at the same time. :confused: who knows.

but i'm betting on the fact that pans are different. obviously.


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OfflineFGL
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Registered: 06/25/05
Posts: 572
Last seen: 10 years, 8 months
Re: 5 liters spawn bags inoculated (pans cyanescens) 4 days ago, still no visible grow [Re: figment]
    #6753823 - 04/06/07 11:15 AM (17 years, 9 months ago)

Many thanks friends!
I wont to look at them for a few days :grin::thumbup:

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