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Offlinedavesj1
Stranger

Registered: 04/11/05
Posts: 260
Last seen: 8 years, 11 months
alternative to LC
    #6741048 - 04/03/07 02:54 AM (17 years, 9 months ago)

I remember reading a post of RR's about using a jar of colonized grain and then mixing some sterile water in with it and then sucking the water back out instead of making a traditional liquid culture.

Can anyone elaborate on this.

Also once the water has been extracted can the grains then be used as spawn or for g2g? Would they need some time to recover? Would they be too wet now?

I have some cyans colonizing right now and would like to try this.

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Invisibleralphroks
humaniform


Registered: 03/25/07
Posts: 553
Loc: Up north passed Alaska
Re: alternative to LC [Re: davesj1]
    #6741069 - 04/03/07 03:33 AM (17 years, 9 months ago)

http://www.shroomery.org/65/Uncle-Lazlos-EZ-Mycelium-Syringe-Tek
<<this might help..I dont see the point, just g2g, or use a small piece
to knock up some honey water if you really want LC. will enuf mycelia
come off the grain? or do you have to blend it?seems like the only diff.
might be.


--------------------
"Please read and learn and relay knowledge not misinformation! Thank you"-hyphae

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Invisiblegeko
Human
Male


Registered: 01/15/07 Happy 18th Shroomiversary!
Posts: 338
Re: alternative to LC [Re: ralphroks]
    #6741130 - 04/03/07 05:01 AM (17 years, 9 months ago)

I tried this and was very optimistic. However, I didn't have much success with it. I knocked some jars (some with as much as a full 10cc syringe of very dense mycellium water) and they took off so poorly and so slowly that I had to dump them. I still have a box of syringes filled with this stuff, but they will probably go down the drain.

Other people may report very different stories. This is just what happened to me.

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OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: alternative to LC [Re: geko]
    #6741591 - 04/03/07 09:53 AM (17 years, 9 months ago)

It works great. I've made a few hundred mycelium syringes this way. You want a fully colonized jar of grains to start with. Add sterilized distilled water to nearly fill up the jar, then shake well, and draw the now myceliated water back out. This is far faster and more dependable than making LC from spores because you can smell the rye jar before using to make sure it isn't contaminated. In addition, a jar of rye colonizes in two weeks, and will make at least 30 syringes. You won't get that performance from karo water.

I agree g2g is a better way to go if you're using grains to inoculate bulk. This tek is for those who still want to use pf tek, as a way to get LC quickly and more safely than with sugar water. The only time I use it is when running experiments on pf cakes, such as the beautiful flush of shiitake I have fruiting now from pf cakes. I've used this tek on cubes and 5 edible species so far, with 100% success. In fact, Roadkill used one of these syringes last November to inoculate some pf cakes as part of my DVD, and those oyster cakes are STILL flushing outdoors. They've produced at least nine or ten flushes so far.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinethemycologist
c21h30o2 inhaler
Registered: 02/28/07
Posts: 213
Loc: spiritual enlightenment
Last seen: 8 months, 1 day
Re: alternative to LC [Re: RogerRabbit]
    #6741774 - 04/03/07 11:10 AM (17 years, 9 months ago)

could this tek be used with a fully colonized pf (brf/verm) jar instead of 100% grains? would the consistency of the cake allow for the water to absorb enough mycelium to make reliable syringes?


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*

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Invisiblegeko
Human
Male


Registered: 01/15/07 Happy 18th Shroomiversary!
Posts: 338
Re: alternative to LC [Re: RogerRabbit]
    #6741992 - 04/03/07 12:18 PM (17 years, 9 months ago)

Quote:

RogerRabbit said:
It works great. I've made a few hundred mycelium syringes this way. You want a fully colonized jar of grains to start with. Add sterilized distilled water to nearly fill up the jar, then shake well, and draw the now myceliated water back out. This is far faster and more dependable than making LC from spores because you can smell the rye jar before using to make sure it isn't contaminated. In addition, a jar of rye colonizes in two weeks, and will make at least 30 syringes. You won't get that performance from karo water.
....
RR




OK, then I have to ask a few questions.... From what you say the colonized grains do not have to be shaken before adding the water, right? As I gather the jar should not be filled to the top with the grains, then it should fully colonize, then I add water filling the empty space, shake, draw the water out... and the grains stay in the jar still compact and white. Is this right?
If so, how much myc water will I need to inoculate a new grain jar after that? And will it take off as fast as with LC?

Sorry for the questions. I have to figure it out, because when I tried it it was a disaster. I took a fully colonized jar, shook the hell out of it, added water, filled syringes with it.... and it didn't work out well.

EDIT: Blutjager answered my questions with his quote. I still don't know why it didn't work for me. I'll try it again.

Edited by geko (04/03/07 01:47 PM)

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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: alternative to LC [Re: geko]
    #6742072 - 04/03/07 12:30 PM (17 years, 9 months ago)

I have had this one book marked fr some time now http://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=6344843&PHPSESSID=&fpart=1#Post6344843 .I cant think of a single bad thing to say about it,I went so far as to shoot a syringe of this stuff into a honey water solution,it got so thick that even the sharp shards of broken glass I use in my baby bottles couldn't break it up....This stuff is amazing:thumbup:

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Offlinedavesj1
Stranger

Registered: 04/11/05
Posts: 260
Last seen: 8 years, 11 months
Re: alternative to LC [Re: geko]
    #6742088 - 04/03/07 12:34 PM (17 years, 9 months ago)

oh jeez, now im gonna do the g2g route. Now i gotta build a glovebox. The shroomery always keeps me busy.

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Invisiblegeko
Human
Male


Registered: 01/15/07 Happy 18th Shroomiversary!
Posts: 338
Re: alternative to LC [Re: davesj1]
    #6742377 - 04/03/07 01:45 PM (17 years, 9 months ago)

Thanks Blutjager. This answers my question. Actually, I did a very simular thing on my first atempt.
It seems that I will have to try this again.

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Offlinedavesj1
Stranger

Registered: 04/11/05
Posts: 260
Last seen: 8 years, 11 months
Re: alternative to LC [Re: geko]
    #6742554 - 04/03/07 02:38 PM (17 years, 9 months ago)

Great Blutjager, that was the post I was originally referring to. But I think im gonna stick w/g2g. Hopefully it will work first time with cyans.

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Invisibleagar
old hand
 User Gallery


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
OG Cultivator
Re: alternative to LC [Re: geko]
    #6743113 - 04/03/07 05:12 PM (17 years, 9 months ago)

Quote:

I took a fully colonized jar, shook the hell out of it, added water, filled syringes with it.... and it didn't work out well.




Part of your mistake, was shaking it FIRST.
Add sterile water, then shake.
That gets you a LOT more myc, in syringes.

How, I do this is.
Make a new wbs/grain jar (quart or 1/2 gallon).
Only fill jar 1/2 way, with wbs/grain.
PC as usual, cool, Innoc & colonize 100%.

Then, inject sterile 3/4 full of sterile water.
THEN, SHAKE the hell out of it.
Then, aspirate out, as many myc syringes, as you want.
Worked GREAT for me.


--------------------

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Invisiblegeko
Human
Male


Registered: 01/15/07 Happy 18th Shroomiversary!
Posts: 338
Re: alternative to LC [Re: agar]
    #6744741 - 04/04/07 12:10 AM (17 years, 9 months ago)

Quote:

agar said:
Quote:

I took a fully colonized jar, shook the hell out of it, added water, filled syringes with it.... and it didn't work out well.




Part of your mistake, was shaking it FIRST.
Add sterile water, then shake.
That gets you a LOT more myc, in syringes.

How, I do this is.
Make a new wbs/grain jar (quart or 1/2 gallon).
Only fill jar 1/2 way, with wbs/grain.
PC as usual, cool, Innoc & colonize 100%.

Then, inject sterile 3/4 full of sterile water.
THEN, SHAKE the hell out of it.
Then, aspirate out, as many myc syringes, as you want.
Worked GREAT for me.




Thanks Agar, that's very clear. I am going to do it again.

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Offlinethemycologist
c21h30o2 inhaler
Registered: 02/28/07
Posts: 213
Loc: spiritual enlightenment
Last seen: 8 months, 1 day
Re: alternative to LC [Re: themycologist]
    #6754506 - 04/06/07 02:28 PM (17 years, 9 months ago)

Quote:

themycologist said:
could this tek be used with a fully colonized pf (brf/verm) jar instead of 100% grains? would the consistency of the cake allow for enough mycelium to transfer to the water to make reliable syringes?




??

thanks!

honey water turned to nasty water . . .


--------------------
"oh god...did you eat all this acid...?
you better pray to god there's some thorazine in that bag,
otherwise you're in bad fucking trouble..." - Raoul Duke

*everything written above is hypothetical, a farce, balderdash*

Extras: Filter Print Post Top
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