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Invisibledeucedbi9
Stranger
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Registered: 10/24/06
Posts: 4,647
Loc: UK
mothership clone so far so good ...despite my amateurish mistakes
    #6730917 - 03/31/07 06:52 AM (17 years, 2 months ago)

first cloning attempt.
using a variation of creamcorns cloning tek.

:http://www.shroomery.org/forums/showflat.php/Number/5874305#5874305

only difference being, i took the biopsy by push the needle up, rather than through the stem,
in an attempt to avoid the contams on the outside of the stipe.



mistake #1. forgot to put some shrapnel,marble etc in the jar,so i couldn't break the myc up.
mistake #2. no provision for GE, so decided to pc some fresh air :rolleyes: and inject into the jar.
obviously anytime you introduce a foreign object into a jar you risk contamination.

the "mother-ship" resisted all attempts to break her up,she seemed to have the consistantcy of a jellyfish.
how do i extract a sample for the wbs i had in mind for her...
then, a few days ago i noticed what looked like wispy smoke when i gave her a swirl, damn.
did i contaminate her with my, probably unnecessary, fresh air injection.

anyway. i decided to inject her with 4ml of 9% h202 into her 500ml of "sugar water" in the hope of dealing with her possible contam.
next day, her beautiful squidgyness had turned into something, resembling phleam :sad: oh well.
i thought sod it,caution to the wind time.
shook the buggery out of her and...she turned into this.



at least i could get a sample of her now.
she was introduced to some newly made wbs.
that was 6 days ago.



so, so far so good.
she's a little slower than two other wbs jars inoculated with a m/spore l/culture on the same day.
but,i'm guessing/hoping, her slow growth is due to the h202 i subjected her to,and that she will make a full recovery.
we shall see...good vibes welcome :mushroom2:


--------------------
whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule

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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: mothership clone so far so good ...despite my amateurish mistakes [Re: deucedbi9]
    #6730962 - 03/31/07 07:29 AM (17 years, 2 months ago)

It is hard to get a good piece of mycelium with a standard needle and is best to split the cap and take a biopsy from the gore of the mushroom,do this in a glove box

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Invisibledeucedbi9
Stranger
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Registered: 10/24/06
Posts: 4,647
Loc: UK
Re: mothership clone so far so good ...despite my amateurish mistakes [Re: Blutjager]
    #6731030 - 03/31/07 08:15 AM (17 years, 2 months ago)

Quote:

Blutjager said:
It is hard to get a good piece of mycelium with a standard needle and is best to split the cap and take a biopsy from the gore of the mushroom,do this in a glove box




yeah, i used a "sawn off" 18 gauge needle for the biopsy.
wanted to avoid the cap all-together.
didn't want to risk accidentally getting spores in the mix.
this was done in open air.in the kitchen.:shocked:

though, as a tilt at sterility.
i washed hand/arms with bleach water and sprayed the area with it,
donned a mask and otherwise practiced sterile techniques.
must say though, i'd be surprised if this all succeeds.:tongue2:


--------------------
whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule

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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: mothership clone so far so good ...despite my amateurish mistakes [Re: deucedbi9]
    #6731591 - 03/31/07 12:00 PM (17 years, 2 months ago)

Even though H2O2 will appear to "melt" mycelium in a liquid solution, it still does leave a good part of it alive, as long as the concentration isn't too strong.

Psilocybe Fanaticus had a tek on H2O2 cloning.

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