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Tooly
Stranger
Registered: 03/30/07
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Last seen: 17 years, 9 months
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requirements for h/poo to colonize with spore inoculation
#6730475 - 03/31/07 01:42 AM (17 years, 9 months ago) |
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I have 12 spawn bags, 200 lbs of horse poop, rye flour, brf, wbs, flax seed and vermiculite. Will these things be enough to properly colonize the poo?
Also, I read a couple posts where they ask if adding brf to poo will allow for simple spore injection, the answer was basically no. It is required for the spores to germinate first or something to that extent. However spawn bags can be used with poo and adding grains to often results in success right?
whats up?
thankyou
Edited by Tooly (03/31/07 02:01 AM)
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6730634 - 03/31/07 02:53 AM (17 years, 9 months ago) |
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What are the spawn bags full of ?? If you have a pressure cooker there is no need to mess with the rye flour, brf, wbs, flax seed or vermiculite
You will likely have no success with inoculating poo,you will need colonized grains to use as spawn,I suggest rye although plenty of people like birdseed as well
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jenerikcairet
cognitivelibertist



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Re: requirements for h/poo to colonize with spore inoculation [Re: Blutjager]
#6730732 - 03/31/07 03:34 AM (17 years, 9 months ago) |
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it literally PAINS me that you have all the shit and dont nkow wtf to do with it.
-------------------- Yes, ordinary water. Laced with nothing more than a few spoons full of LSD- professor farsnworth
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6730838 - 03/31/07 05:41 AM (17 years, 9 months ago) |
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-------------------- Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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Hobosapien
Mushmage


Registered: 02/25/07
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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6730960 - 03/31/07 07:28 AM (17 years, 9 months ago) |
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Quote:
Tooly said:
Also, I read a couple posts....
yup, read a couple more...
ACTUALLY, even though this site is a BOMB ass resource, never underestimate having a big fad mushroom growing BIBLE at your bedside table, or under your pillow if your down with osmosis....
Mr. Stamets has one or two of said books available.
-------------------- [img][url=http://www.shroomery.org/forums/files/07-13/511364480-allseeingeye.gif]
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Tooly
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Last seen: 17 years, 9 months
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Re: requirements for h/poo to colonize with spore inoculation [Re: Roadkill]
#6731436 - 03/31/07 11:09 AM (17 years, 9 months ago) |
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Know any good ratios when adding the grain to the h/poo?
"What are the spawn bags full of ?? If you have a pressure cooker there is no need to mess with the rye flour, brf, wbs, flax seed or vermiculite
You will likely have no success with inoculating poo,you will need colonized grains to use as spawn,I suggest rye although plenty of people like birdseed as well "
there are teks for loading spawn bags and show success, no spawn used. However I am looking for what ratio to add my grains to my poo.
"it literally PAINS me that you have all the shit and dont nkow wtf to do with it. "
assuming you were talking to me, although you didn't reply to me, I know it al can be used I just dont know the RATIO!!!!!!!
PLEASE DOES ANYONE KNOW A RATIO OF GRAIN TO H/POO?!!!!!!!!!!!!!
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agar
old hand



Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6731468 - 03/31/07 11:21 AM (17 years, 9 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/6347213#Post6347213
Optimal colonized spawn ratio to a bulk substrate is; 1 to 4, or 1 to 5 = 20% to 25%.

It takes considerable skill & expertise to do LC to bulk. In bags. Don't try it now.
You will do far better, colonizing wbs/grain jars. Then, use THAT as spawn to bulk substrate.
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Tooly
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Re: requirements for h/poo to colonize with spore inoculation [Re: agar]
#6731487 - 03/31/07 11:25 AM (17 years, 9 months ago) |
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no I wasn't looking for a spawn to h/poo ratio
I have spawn bags and straw and h/poo
H/poo wont colonize with spore injection so I need to add grains right?
What ratio?
thankyou
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agar
old hand



Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6731505 - 03/31/07 11:30 AM (17 years, 9 months ago) |
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You need to add COLONIZED grains. Adding straight grain to h/poo. Then, injecting with spore solution. Will get you nowhere. So there IS NO RATIO, for what you are asking.
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Tooly
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Re: requirements for h/poo to colonize with spore inoculation [Re: agar]
#6731516 - 03/31/07 11:32 AM (17 years, 9 months ago) |
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why are spawn bags sold?
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Tooly
Stranger
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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6731524 - 03/31/07 11:34 AM (17 years, 9 months ago) |
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Could I use straw and add grains to it?
I was going to do that but I don't have a weedwacker and it was taking forever cutting that shit in 1-3 inch pieces
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Tooly
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Re: requirements for h/poo to colonize with spore inoculation [Re: agar]
#6731544 - 03/31/07 11:42 AM (17 years, 9 months ago) |
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bro I have 12 spawn bags and 12 syringes how can I colonize these bags???
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agar
old hand



Registered: 11/21/04
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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6731557 - 03/31/07 11:48 AM (17 years, 9 months ago) |
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 To cultivate mushrooms in. Once YOU KNOW HOW.
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Tooly
Stranger
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Re: requirements for h/poo to colonize with spore inoculation [Re: agar]
#6731572 - 03/31/07 11:51 AM (17 years, 9 months ago) |
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A bulk even half as good as that would be awesome 
I just need a formula for colonizing spawn bags with SPORE inncoulation, that's all....
Can you help me out?
Edited by Tooly (03/31/07 11:57 AM)
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agar
old hand



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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6731602 - 03/31/07 12:05 PM (17 years, 9 months ago) |
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The only time I have had success with innoc'ing bags. Was using LC (liquid culture) & MycoNitro substrate. (MycoNitro is a very specialized compost & not commercially available)
I colonized jars of it, first, as a test.
 Before even attempting bags.
 Learn grain spawn, in jars.
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Tooly
Stranger
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Re: requirements for h/poo to colonize with spore inoculation [Re: agar]
#6731640 - 03/31/07 12:23 PM (17 years, 9 months ago) |
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there is a tek here for loading spawn bags (with poo) but simply says "final substrate" is doesnt clarify what to make that final substrate of
H/poo can be used with spawn bags, if modified
Nobody seems to know, insisting they have to colonize on grains first
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agar
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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6731659 - 03/31/07 12:35 PM (17 years, 9 months ago) |
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RogerRabbit
Bans for Pleasure


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Re: requirements for h/poo to colonize with spore inoculation [Re: Tooly]
#6731660 - 03/31/07 12:35 PM (17 years, 9 months ago) |
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What part of "colonize grains from spores, then use colonized grains to inoculate manure" are you failing to understand?
I've seen a grow or two where uncolonized grains were mixed with manure and injected with spores, but that is a recipe for failure and is not sound mycological technique. The reason is that grains require sterilization, and manure does best with pasteurization. They also each have different moisture requirements and different gas/air exchange requirements, so mixing them uncolonized is not recommended. Follow the advice agar gave above if you want to succeed. Nobody wants to help you fail. RR
-------------------- Download Let's Grow Mushrooms
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
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Tooly
Stranger
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Re: requirements for h/poo to colonize with spore inoculation [Re: RogerRabbit]
#6731666 - 03/31/07 12:38 PM (17 years, 9 months ago) |
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Alright I guess I'll move back to grains and the pf tek
Since I have rye flour, wbs, .ect and no use now
Can I modify the pf tek with these grains?
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Tooly
Stranger
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Last seen: 17 years, 9 months
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Re: requirements for h/poo to colonize with spore inoculation [Re: agar]
#6731675 - 03/31/07 12:41 PM (17 years, 9 months ago) |
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I've read that tek a million times and still it says nothing on WHAT to load them with
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