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Invisiblefarva86
I dont want alarge farva. Iwant aleeder-a-cola
Male

Registered: 02/23/07
Posts: 267
Loc: MIddle of Nowhere, AR
murky LC-PIC
    #6730250 - 03/31/07 12:26 AM (17 years, 2 months ago)

i inoculated my LC a couple of days ago using 1 packet of sugar in the raw and ~220ml water(Agar's turbinado tek). i PCed it for ~15-20 mins. the only thing ive been able to find suggests a bacterial contam. heres the link: http://www.shroomery.org/forums/showflat.php/Number/5356241#5356241

the whole thing is murky and damned impossible to see through. heres a pic:

i guess i just wanna know for certain that its contamed b4 i toss it.


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Eddie Money doesn't have to put up with this shit.
The wonder has a name: shrooms.

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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: murky LC-PIC [Re: farva86]
    #6730593 - 03/31/07 02:35 AM (17 years, 2 months ago)

Hate to tell you this but I do believe its time to dump that and start over,you could shoot something up with it just to find out for certain but I would make up a test jar or two so as not to waste a ton of spawn

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Invisiblefarva86
I dont want alarge farva. Iwant aleeder-a-cola
Male

Registered: 02/23/07
Posts: 267
Loc: MIddle of Nowhere, AR
Re: murky LC-PIC [Re: Blutjager]
    #6730720 - 03/31/07 03:29 AM (17 years, 2 months ago)

im just gonna toss it.  its only been a couple of days so there wont be enough myc in it for anything and im not interested in growing contams.

in the link above hyphae states that putting it in a pot of boiling water for a minimum of 5 minutes is sufficient.  will 5 mins be able to leave me worry free or do i need to do it longer? basically how long for peace of mind?

will 1cc from a spore syringe be enough to inoculate an lc with ~220ml?

also im pretty sure where i fucked up on the jar above.  it wasnt the spore syringe because the jars i did several days ago are growing great, with the exception of 1:mad2: that i think im gonna blow up.  when i PCed it i only waited until the pressure equalized, not until it hit room temp, thus creating a vacuum.  i ousted the air and put some tyvek over the lid with a rubber band (something i read here on the shroomery) and popped it open.  i think with only the rubberband to keep the tykey on is how contams got in.  but if i had waited like i should have this never would have been a problem.  oh well, live and learn.


--------------------
Eddie Money doesn't have to put up with this shit.
The wonder has a name: shrooms.

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Invisibleagar
old hand
 User Gallery


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: murky LC-PIC [Re: farva86]
    #6731026 - 03/31/07 08:11 AM (17 years, 2 months ago)


If your LC is murky, to start.
Sadly, bacteria bloomed in it quickly.
Either it was not properly sterilized.
The lid, filter or port is not adequate.
The syringe had bacteria in it.
Or, some attached to the needle, during injection.
One way or another, bacteria got in.:mad2:


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