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OfflineHUBSonDUBS
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Microwave jar of water to sterilize and create syringes?
    #6726043 - 03/29/07 08:05 PM (17 years, 9 months ago)

I was going to fill a mason jar with tap water put saran wrap on the top and place in the microwave for 3 minute. After it cooled place a print inside and suck up into a syringe. Does this sound good enough?


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InvisibleLayYouIn
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Re: Microwave jar of water to sterilize and create syringes? [Re: HUBSonDUBS]
    #6726064 - 03/29/07 08:12 PM (17 years, 9 months ago)

wont the saran wrap melt?

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OfflineHUBSonDUBS
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Re: Microwave jar of water to sterilize and create syringes? [Re: LayYouIn]
    #6726075 - 03/29/07 08:15 PM (17 years, 9 months ago)

Nope.


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InvisibleKilroyMilosevik
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Re: Microwave jar of water to sterilize and create syringes? [Re: LayYouIn]
    #6726079 - 03/29/07 08:16 PM (17 years, 9 months ago)

nah, saran wrap won't melt... Yea I assume if you bring the water in the jar to a boil for a few minutes it would be perfectly fine... just my opinion; I'm no expert by any means

Edit: Actually, I don't think you would even need to cover it if you're going to boil it


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-The door.
-The door is closed.
-Why is the door closed?
        *Gasps*
-Why DOES the door close!?

Edited by KilroyMilosevik (03/29/07 08:17 PM)

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InvisibleLayYouIn
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Re: Microwave jar of water to sterilize and create syringes? [Re: LayYouIn]
    #6726124 - 03/29/07 08:30 PM (17 years, 9 months ago)

weird...i never knew saran wrap could be microwaved.

personally, i think it would be easier to fill the syringes with water, wrap in tin foil, and simmer/PC for and hour...then just squirt the water into the print and suck it back in. maybe use an extra syringe so that you get full ones.

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InvisiblePoC
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Re: Microwave jar of water to sterilize and create syringes? [Re: LayYouIn]
    #6726227 - 03/29/07 09:01 PM (17 years, 9 months ago)

I've microwaved karo/water solution in a plastic tupperware that had a small nail-sized hole in it covered entirely w/ saran wrap to make a liquid culture. Wiped the saran wrap over the hole down w/ alcohol, shot spores in, left it to grow myc. w/ micropore tape over the hole, and took it back out. I've also used it to just put spores in there and draw out karo/water/spore solution into syringes and let it grow but it's less effective b/c there's no gas exchange.

Worked just fine but if you have a pressure cooker then you should just get pumped for a bit and forget about being lazy :P


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OfflineRogerRabbitM
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Re: Microwave jar of water to sterilize and create syringes? [Re: PoC]
    #6726247 - 03/29/07 09:07 PM (17 years, 9 months ago)

Just pressure cook a jar of distilled water with a filter on it and don't take chances. Microwaves are usually the filthiest appliance in a house because there's no way to clean up inside the area where the fan circulates air past the magnetron tube and back to the chamber. I don't consider microwaves suitable for any mycology project. Others disagree, but that's my stand. I hate failure, and go to great lengths to avoid it.
RR


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Offlinemikemushroom
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Re: Microwave jar of water to sterilize and create syringes? [Re: RogerRabbit]
    #6726527 - 03/29/07 10:15 PM (17 years, 9 months ago)

Another option-

Go to the grocery. Look in the baby food aisle. Find the Pedialyte sterile water.

I have not tried this, but I do not see why it would not work.
__ __ __

I may try an experiment in the near future:

Get the Pedialyte. Swab the foil seal with 70% alc. Insert syringe through seal. Suck up the appropriate amount of water per syringe. Inject the water into a sterile jar w/ lid and spores (from print) then draw up the solution through the syringe.

I should mention the lid of the jar would need to have a large enough hole for a 16-18G needle.

MM


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Want to be of the shaman mind? Carlos Castaneda is the godfather of the New Age Movement which is based off the peyote & shroom eating natives in Mexico.

Read his library of books to expand your mind.

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Offlinewiggles
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Re: Microwave jar of water to sterilize and create syringes? [Re: mikemushroom]
    #6726536 - 03/29/07 10:18 PM (17 years, 9 months ago)

Syringes would not work. You cant put a metal needle in a microwave or else
:microwave:


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You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye.
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Offlinemikemushroom
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Re: Microwave jar of water to sterilize and create syringes? [Re: wiggles]
    #6726555 - 03/29/07 10:23 PM (17 years, 9 months ago)

Uhhh...no one suggested putting a needle in the microwave.:crazy:


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Want to be of the shaman mind? Carlos Castaneda is the godfather of the New Age Movement which is based off the peyote & shroom eating natives in Mexico.

Read his library of books to expand your mind.

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Offlinewiggles
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Re: Microwave jar of water to sterilize and create syringes? [Re: mikemushroom]
    #6726564 - 03/29/07 10:25 PM (17 years, 9 months ago)

The title mentioned syringes, so i figured :smile:


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You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye.
Hunter S. Thompson

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Invisibleagar
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Re: Microwave jar of water to sterilize and create syringes? [Re: HUBSonDUBS]
    #6726607 - 03/29/07 10:47 PM (17 years, 9 months ago)

It is not quite that simple.
I did just prepared an LC, in the microwave, though.:grin:

Going to innoc, as soon as cooled to room temps.

And, for anybody who might argue.
A microwaved LC doesn't work.
Here is a test jar from last week.

It's all in the lid & knowing how.


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Edited by agar (03/29/07 10:57 PM)

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InvisiblePoC
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Re: Microwave jar of water to sterilize and create syringes? [Re: agar]
    #6726699 - 03/29/07 11:10 PM (17 years, 9 months ago)

Hrm so I don't want to throw this thread off topic but agar has a note to not PM about mush/cult questions which I guess this falls under...

Agar, what's your method on getting good pictures of LC? I had success before by putting it in front of a black tray with light shining in on the myc but it has lately been hit or miss getting good focus on the myc instead of the jar or something else.


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Invisibleagar
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Re: Microwave jar of water to sterilize and create syringes? [Re: PoC]
    #6726720 - 03/29/07 11:15 PM (17 years, 9 months ago)


Focus a lamp to the side, or under it.
Take about 10 shots & pick a good one.


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InvisiblePoC
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Re: Microwave jar of water to sterilize and create syringes? [Re: agar]
    #6726768 - 03/29/07 11:31 PM (17 years, 9 months ago)

Haha that simple huh?  Well I just took another stab at this and here's the results.  Turned out way better than what I had been getting recently though...wonder why.

   
(Acadian Coast)
And just a shot of the jar the LC is in which is about the clarity I normally get from a picture of an LC lol:



Anyhow thanks for the response...back on topic! :smile:


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Edited by PoC (03/29/07 11:35 PM)

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OfflineJohn0809
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Re: Microwave jar of water to sterilize and create syringes? [Re: PoC]
    #6727378 - 03/30/07 08:38 AM (17 years, 9 months ago)

i sterlize my needle and MEtal tips in the Microwave all the time..the metal neele is too little of metal to actually cause problems...

Honestly tho I sterilize the whole needle in the microwave in a tupperware container in about 2-3 inches of water with the lid 3/4 the way secured run the microwave for about 5 mins...

JS


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OfflineRogerRabbitM
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Re: Microwave jar of water to sterilize and create syringes? [Re: John0809]
    #6727480 - 03/30/07 09:20 AM (17 years, 9 months ago)

It should be noted that if you're using karo, you don't need to sterilize at all. Mrs rabbit has a jar of Karo that she's been using for nearly a year, opening it in the kitchen and sometimes leaving it open for the whole time she's cooking and it has zero contamination in it. When I say don't microwave LC, I'm referring to using malt extract/yeast, which should be PC'd for half an hour.

As for distilled water for syringe making, as agar said, a good filtered lid is the key, so anything that makes the water boil to kill off bacteria is going to work. Personally, I pc mine.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinemikemushroom
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Re: Microwave jar of water to sterilize and create syringes? [Re: John0809]
    #6728350 - 03/30/07 02:07 PM (17 years, 9 months ago)

Quote:

John0809 said:
i sterlize my needle and MEtal tips in the Microwave all the time..the metal neele is too little of metal to actually cause problems...

Honestly tho I sterilize the whole needle in the microwave in a tupperware container in about 2-3 inches of water with the lid 3/4 the way secured run the microwave for about 5 mins...

JS




Sterilizing needles in the MW? With all due respect, this is the stupidest thing I have ever heard.

Put a staple in the microwave and see what happens.

FYI: Just to prove this point, I stuck an extra needle in a MW (in my garage, of course). It took 1/10 of a second to start making a ZZZZZZ sound and a flash of electricity.

I call bull shit.

MM


--------------------
Want to be of the shaman mind? Carlos Castaneda is the godfather of the New Age Movement which is based off the peyote & shroom eating natives in Mexico.

Read his library of books to expand your mind.

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InvisiblePoC
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Re: Microwave jar of water to sterilize and create syringes? [Re: mikemushroom]
    #6728527 - 03/30/07 02:55 PM (17 years, 9 months ago)

D'no...it's probably not the smartest idea but it could probably work without blowing anything up. You ever put a CD in your microwave just to see the lightning show? That doesn't blow up your microwave...at least it hasn't blown up any of my old friends'


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InvisibleBlutjager
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Re: Microwave jar of water to sterilize and create syringes? [Re: PoC]
    #6728897 - 03/30/07 04:49 PM (17 years, 9 months ago)

Quote:

PatternOfChaos said:
D'no...it's probably not the smartest idea but it could probably work without blowing anything up.  You ever put a CD in your microwave just to see the lightning show?  That doesn't blow up your microwave...at least it hasn't blown up any of my old friends'




I thought I was the only one who has done this:lol:

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