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OfflineNickio
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First grow failed, questions regarding verm and inoculation
    #6724872 - 03/29/07 02:51 PM (17 years, 3 months ago)

Background: I've posted here a few times about my first grow, and it seems that I failed. I believe the reason was because I used a pot to sterilize my jars. None of them got contaminated, but they all cracked, and it seems that the growth has stopped. I haven't discarded these jars yet, as I'm hoping for a miracle. If any experts have any input about the thread below please let me know =)

More info: http://www.shroomery.org/forums/showflat.php/Number/6720723#6720723

Pic of one of my stalled jars:



So I'm ready to bite the bullet, get a PCer, order another syringe and try again. I'm really determined to get this to work.

First question: vermiculite. I was able to find vermiculite at the local Lowes, but it isn't chunky, its very fine and powdery. The cracked jars seemed to grow with it though, but I thought I should ask if this is okay to use before using another syringe. Pics of the vermiculite I'm using below:







Second question: Do I need to order another syringe? Can I use pieces of the cakes I have now to inoculate other jars which would save me money and time. Just wondering!

Let me know what you guys think, thanks for reading.


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Offlineatomic1
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Re: First grow failed, questions regarding verm and inoculation [Re: Nickio]
    #6725509 - 03/29/07 05:14 PM (17 years, 3 months ago)

That verm works fine. Just be sure not to compact your jars. Leave them as loose and fluffy as possible. If you don't have anymore spore solution then go ahead and order another syringe. The pic of that jar doesn't look very promising. Oh, and definitely get a pc. You'll be glad you did in the long run.

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OfflineNickio
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Re: First grow failed, questions regarding verm and inoculation [Re: atomic1]
    #6726850 - 03/30/07 12:15 AM (17 years, 3 months ago)

bump


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Offlinejenerikcairet
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Re: First grow failed, questions regarding verm and inoculation [Re: Nickio]
    #6727204 - 03/30/07 06:41 AM (17 years, 3 months ago)

im not sure if transfer mycellium like you can on a grain to grain transfer works as well or at all on pf jars, because im not experienced with vemiculite. i can however tell you that:


A- that vermiculite is fine for pf jars

B- youd be better of not trying to save time and money , spend a little bit on a new syringe, wait a f ew days for it because if you tried to do what youre talking about, contams are very probable on your first grow. also it might not even work. idk, personally i would just order a new syringe and be patient even though thats really hard to do in the beginning.


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Yes, ordinary water. Laced with nothing more than a few spoons full of LSD- professor farsnworth

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OfflineSpekOner
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Re: First grow failed, questions regarding verm and inoculation [Re: jenerikcairet]
    #6727223 - 03/30/07 07:09 AM (17 years, 3 months ago)

i agree with the posts above^^

for my input i would say the poor jar in the pic looks VERY dry. make sure your using the right amount of water... i use 2 parts verm, 1 part BRF and 1 part water.. are they being kept at 80 degree temp?.. and the last thing, if a jar cracks while being sterilized, DO NOT inoculate!

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OfflineJohn0809
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Re: First grow failed, questions regarding verm and inoculation [Re: SpekOner]
    #6727447 - 03/30/07 09:07 AM (17 years, 3 months ago)

add some sterile water and give them a little air exchange by opening the jars in a glove box thats been clean and lysoled..


JS


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Check out my Site!

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OfflineRogerRabbitM
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Re: First grow failed, questions regarding verm and inoculation [Re: John0809]
    #6727454 - 03/30/07 09:11 AM (17 years, 3 months ago)

Why am I the only one that can see that horrible mold at the bottom of the jar where the glass cracked?

You need to toss that thing out. The verm is fine. Next time, elevate your jars off the bottom of the kettle so the glass doesn't crack, which allows contaminants to enter.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinejagg
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Re: First grow failed, questions regarding verm and inoculation [Re: RogerRabbit]
    #6728089 - 03/30/07 01:03 PM (17 years, 2 months ago)

Spore syringe: $20.00
Pressure cooker: $45.00
Case O jars: $8.00
Bag O vermiculite: $5.00
Bag O brown rice: $5.00
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First flush of shrooms, priceless!!!

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Offlinekyleda1kid
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Registered: 03/27/07
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Re: First grow failed, questions regarding verm and inoculation [Re: jagg]
    #6728238 - 03/30/07 01:36 PM (17 years, 2 months ago)

Lol  :blazed: That was funny, but then again, im blazed  :blazed:

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InvisibleBlutjager
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Re: First grow failed, questions regarding verm and inoculation [Re: kyleda1kid]
    #6728999 - 03/30/07 05:20 PM (17 years, 2 months ago)

I would be really REALLY surprised if you could salvage usable mycelium from that jar with out doing agar work,a grain to grain transfer from any jar with contamination is suicide:slitwrist::suicide:

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Offlinejash420
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Re: First grow failed, questions regarding verm and inoculation [Re: Blutjager]
    #6729140 - 03/30/07 06:05 PM (17 years, 2 months ago)

yea man just toss it, take things slower. RR I was wondering if I saw that thing contam'd like crazy also, obviously the eyes were not as deceiving as I thought. Definitly get a PC it will be your best friend in this hobby. Cleanliness is next to godliness in this hobby.


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I love poo, any friend of my mushrooms is a friend of mine..

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Offlinewiggles
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Re: First grow failed, questions regarding verm and inoculation [Re: jash420]
    #6729154 - 03/30/07 06:11 PM (17 years, 2 months ago)

The jar cracked, and they got contammed. Thats why they stalled. It also looks like they were extremely dry, which gave the opportunistic contam the chance to spread.

Next time make sure to put a cloth underneath the jars if you boil - you don't want them directly exposed to heat. If you want to succeed, you really should spend the cash on a decent pressure cooker. Even if you don't take up this hobby, you'll be able to cook some damn tasty meals.

Plus remember to have the pf mix be just wet enough that if you squeeze it water will drip out, but it won't drip if you just hold it. Also make the mix jar by jar. If you try to make it all at once you tend to have wet and dry spots.


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You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye.
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OfflineRogerRabbitM
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Re: First grow failed, questions regarding verm and inoculation [Re: wiggles]
    #6729172 - 03/30/07 06:17 PM (17 years, 2 months ago)

Make pf tek by the recipe. 1 cup of brown rice flour, 1 cup of water, 2 cups of verm. That will fill five 1/2 pint jars. If you can squeeze water out of it, it's too wet.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinemushmonster
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Re: First grow failed, questions regarding verm and inoculation [Re: RogerRabbit]
    #6729893 - 03/30/07 10:37 PM (17 years, 2 months ago)

verm is fine , get pressure cooker and next time u make up jars get some tyvecpaper from home-depot and cut a square 2in. wider than your jars top . and leave out center of jar lid and replace with tyvec paper for air exchange ,and so you don't blow your jars away when you pc air tite lid causes pressure which cracks your jar or you didn't leave jars tyo cool down before takin them out and cool air cracked them


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