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Kamin
Registered: 02/04/07
Posts: 449
Last seen: 12 years, 5 months
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Rye bags show no progress
#6697506 - 03/21/07 10:53 PM (17 years, 12 days ago) |
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I'm using two rye bags from mushbox.com. Both were inoculated with about 2 cc's of a spore syringe. After incubating for 2 weeks at 80 degrees, they showed zero progress. I waited another week and still nothing. Not knwoing what to do, I tried innoculating them again a little less than a week ago with about 3cc's each. They show no signs of contams, which is good, but I also don't see any mycelial progress. What could be going on?
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Tobolam
Cow fieldlawnmower
Registered: 02/26/07
Posts: 63
Last seen: 16 years, 11 months
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Re: Rye bags show no progress [Re: Kamin]
#6697547 - 03/21/07 11:04 PM (17 years, 12 days ago) |
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The bags suck? Well check it to make sure there is enough moisture as they could have dried out through a filter, also make sure it's really 80 degrees and not 90 or so, because too high could prevent growth
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Kamin
Registered: 02/04/07
Posts: 449
Last seen: 12 years, 5 months
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Re: Rye bags show no progress [Re: Tobolam]
#6697583 - 03/21/07 11:14 PM (17 years, 12 days ago) |
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the bags definitely arent too dry, if anything, i was a little worried about high moisture content. And I just checked again, the incubator is around 80.5 degrees.
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acoustick1tty
Stranger
Registered: 03/18/07
Posts: 5
Last seen: 17 years, 12 days
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Re: Rye bags show no progress [Re: Kamin]
#6697664 - 03/21/07 11:33 PM (17 years, 12 days ago) |
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I don't use bags, but I've had a problem attempting to grow on rye. I'm pretty sure that this is due to fungicide in my supposedly "organic" rye flour. The worst part is that I really would like to nail the company on this, but can't, for obvious reasons.
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geko
Human
Registered: 01/15/07
Posts: 338
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How big are these bags? I am not positive on this, but 2cc for a decent sized bag seems too little to me. Besides, if it looks damp, there is probably bacterial contamination. Smell through the filter. If it smells soar, fermenting, then you can not save the bags. Anyway - 2 weeks with no progress at incubation temperature and damp grains means 99% no chance. I would start over. By now you would have had them 100% colonized if everything was right.
Very sorry for the bad news. I know how it is.
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Kamin
Registered: 02/04/07
Posts: 449
Last seen: 12 years, 5 months
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Re: Rye bags show no progress [Re: geko]
#6698610 - 03/22/07 09:14 AM (17 years, 11 days ago) |
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Yeah, makes me thnk something was wrong with the syringes or the bags. The other bag that was knocked up with z-strain in the same fashion had no problems. The bags are the 3 lb bags. On mushbox they have a video that explains how much to put in, and they said 2-3 cc's so I'm a little confused as to why nothing happened lol.
Edited by Kamin (03/22/07 09:16 AM)
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Zepplin
journeyman
Registered: 12/31/01
Posts: 732
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Re: Rye bags show no progress [Re: Kamin]
#6698771 - 03/22/07 10:32 AM (17 years, 11 days ago) |
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Quote:
Kamin said: I'm using two rye bags from mushbox.com. Both were inoculated with about 2 cc's of a spore syringe. After incubating for 2 weeks at 80 degrees, they showed zero progress. I waited another week and still nothing. Not knwoing what to do, I tried innoculating them again a little less than a week ago with about 3cc's each. They show no signs of contams, which is good, but I also don't see any mycelial progress. What could be going on?
Well, for one..your temperature is way too high. Incubate at room temperature, esp. with bags. Remember, the bags will generate heat so the temperature inside the bag is higher than ideal. Also the higher temp. is an ideal breeding ground for bacteria.
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