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VampireSlayer
killing ghosts,zombies andvampires forlife



Registered: 08/26/06
Posts: 2,529
Last seen: 17 years, 3 months
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question about cloning
#6668110 - 03/14/07 09:43 AM (17 years, 10 months ago) |
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I don't understand it. Since cloning isn't isolating a strain, the how does it work. If you clone a mushroom lets say using liquid culture, are you getting a single strain of mushroom or is the mycellium in the lc still a bunch of different strains as if you used multispore innocultion?
-------------------- I Don't come to fight flesh and blood but spiritual wickedness in high and low places
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Premedman1
Assistant to the insistent



Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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I can see how this would be confusing. Cloning is not really "isolating" because there's nothing to isolate away from. When you clone, the resulting mycelium will have the same genetics as the parent fruit. It will be comprised of one single "sub-strain". Isolation is done on agar, where you are isolating away contaminates or less desirable "sub-strains". I hope this helps, probably just made it more confusing.
-------------------- Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: question about cloning [Re: Premedman1]
#6668306 - 03/14/07 10:43 AM (17 years, 10 months ago) |
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In addition, when multispore inoculation is used, more than one strain often 'collaborate' to produce fruits, so cloning isn't always a guarantee of a single strain. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Premedman1
Assistant to the insistent



Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: question about cloning [Re: RogerRabbit]
#6668333 - 03/14/07 10:54 AM (17 years, 10 months ago) |
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I don't understand RR. So a single fruit can contain genetics from more than one "parent" strain, and each's attributes could present themselves in future grows from a single clone? Could you please explain?
-------------------- Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: question about cloning [Re: Premedman1]
#6668366 - 03/14/07 11:04 AM (17 years, 10 months ago) |
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Paul does a much better job of explaining it in GGMM than I can, but yes, with multispore inoculation it sometimes happens. I've cloned tissue from fruits before and observed multiple sectors branch out. I don't mean different mycelium types, but actual sectors of non-compatible strains. It's fairly rare because most strains combine via anastomosis prior to flushing, but it does happen sometimes, and it's much more common when cloning wild fruits. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Premedman1
Assistant to the insistent



Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: question about cloning [Re: RogerRabbit]
#6668392 - 03/14/07 11:14 AM (17 years, 10 months ago) |
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I see, thank you. I never knew that was possible. I hope this helps the original poster. +5 for you Roger.
-------------------- Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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VampireSlayer
killing ghosts,zombies andvampires forlife



Registered: 08/26/06
Posts: 2,529
Last seen: 17 years, 3 months
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Re: question about cloning [Re: Premedman1]
#6668622 - 03/14/07 12:19 PM (17 years, 10 months ago) |
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yes thank you RR. I am so glad you are around to share your knowlege.
-------------------- I Don't come to fight flesh and blood but spiritual wickedness in high and low places
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Civ
Pinning



Registered: 10/14/04
Posts: 2,537
Loc: California
Last seen: 3 months, 22 days
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Quote:
I am so glad you are around to share your knowlege
Or since he has answered this, and 100s have asked this already- you could have found your answers via the search engine.
-------------------- "...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..." -Agar
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fahtster
Now With 33%More Faht


Registered: 06/17/06
Posts: 9,716
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Re: question about cloning [Re: Civ]
#6668957 - 03/14/07 01:50 PM (17 years, 10 months ago) |
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reposting info never hurts in forums this size, esp. since there are people that love helping out. I don't know how many times I've learned something that I've wondered about by just reading what others have to say in various posts that I was wasn't even looking for... like this thread for example... I didn't know that one fruitbody could be from two parent strains... I don't think I would ever have looked for that info but there it is. 
fahtster
Edited by fahtster (03/14/07 02:00 PM)
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deucedbi9
Stranger


Registered: 10/24/06
Posts: 4,696
Loc: UK
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Re: question about cloning [Re: fahtster]
#6669431 - 03/14/07 04:08 PM (17 years, 10 months ago) |
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Quote:
fahtster said: reposting info never hurts in forums this size, esp. since there are people that love helping out. I don't know how many times I've learned something that I've wondered about by just reading what others have to say in various posts that I was wasn't even looking for... like this thread for example... I didn't know that one fruitbody could be from two parent strains... I don't think I would ever have looked for that info but there it is. 
fahtster
its like the open university of mycology  www.open.ac.uk/ don't know if they do mycology yet 
does this look like it has some "sectors" to anyone?
-------------------- whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.
even though I'm feeling good
Something tells me I'd better activate my prayer capsule
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