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 MinkCoat
Stranger
Registered: 05/14/02
Posts: 6
Loc: 25s, 60w
Last seen: 22 years, 4 months
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 Jar Salvage 
#666216 - 06/06/02 02:41 PM (22 years, 6 months ago) |
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I wasn't planning on posting here at all, since I've been reading through A LOT of posts and have read a lot about mushies, but of course, things had to get hairy, and now I don't know what I'm doing...any help would be greatly appreciated. 
Here's my 'dilema'...I innoculated 8 jars with B+ a week ago. Yesterday I checked the jars and many of them were fairly dry but one or two had little dots of mycelium - I hadn't taped the holes well enough, but thought they might need some air anyway. I live in a very dry place so I guess they just dried up a little too fast - and it's very hard to create a high humidity storage area.
To make a long story short, I sterilized my working area with lysol and 70% peroxide. Then with clean hands emptied the jar contents into my pressure cooker (sterilized of course), and added a peroxide solution to moisten the substrate. There was a tiny bit of mycelium in one of the jars (that I could see), so I figured it would start to colonize even with the peroxide solution (something like 1% isopropyl). I then sealed up the pressure cooker to prevent it from drying out like last time... I have read that in dry areas contams are easier to aviod, but I'm not sure. Will this work?
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"Experience is the only form of knowledge...
and words have no intrinsic value. Recorded knowledge is false, and is pulling us farther away from true knowledge." - The homeless guy outside Safeway.
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 phrozendata
Carpal Tunnel
Registered: 04/23/00
Posts: 5,015
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 Re: Jar Salvage [Re: MinkCoat]
#666406 - 06/06/02 05:05 PM (22 years, 6 months ago) |
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Doesn't sound too good...
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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley
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cbull
enthusiast
Registered: 05/11/02
Posts: 363
Last seen: 21 years, 29 days
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Re: Jar Salvage [Re: MinkCoat]
#666458 - 06/06/02 05:40 PM (22 years, 6 months ago) |
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well i dunno really but if that jar comes to life and u see contams this is what i do i empty the jar out in a glove box take the pice of mycelium the contam didnt get to yet and put it in a new jar that i sterlized with a pressuar cooker this way even if the jar gets contamed make a new jar and it will have the moisture and this way u can put tinfoil inder the cap with no holes so no water can escape
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FAPA - Member Since -5-21-2002
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MinkCoat
Stranger
Registered: 05/14/02
Posts: 6
Loc: 25s, 60w
Last seen: 22 years, 4 months
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Re: Jar Salvage [Re: cbull]
#666541 - 06/06/02 06:41 PM (22 years, 6 months ago) |
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thanx for the advice,
actually i guess i didn't actually say what the problem was with the jars...they almost dried up. if I left them for another 2-3 days i'm sure they would be completely dried up and useless. i would have just poured water through the holes in the top but the substrate wasn't having that... so i emptied everything into a pot and poured water over them...sounds bad but i made sure everything was a sterile as possible. didn't want to waste the substrate/spores. if anyone else has had to do something like this how did it go?
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"Experience is the only form of knowledge...
and words have no intrinsic value. Recorded knowledge is false, and is pulling us farther away from true knowledge." - The homeless guy outside Safeway.
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Drummer
CelestialMechanic
 
Registered: 08/15/00
Posts: 708
Loc: SE Michigan
Last seen: 1 year, 7 months
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Re: Jar Salvage [Re: MinkCoat]
#666555 - 06/06/02 06:49 PM (22 years, 6 months ago) |
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The only thing I ever done was inject a few cc's of water with a syringe when mine dried up. Of course, I did use sterile water and the same precautions as I would when innoculating. I had excellent results. Since then I fixed my incubator and never had another problem.
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People don't want other people to get high, because if you get high, you might see the falsity of the fabric of the society we live in.
-Ken Kesey
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