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Offlinephishyfriend
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Registered: 05/14/02
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CLONING in a hurry?????
    #665799 - 06/06/02 11:38 AM (15 years, 25 days ago)

Could someone tell me how to clone i have some puerto ricans(pardon spelling)and there pinning outside and i wanna bring them in. is there a way to do this fast? and is there a way to do this without a petri dish. could you give me exact measurments if needed please some one help.


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OfflineTheShroomHermit
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Re: CLONING in a hurry????? [Re: phishyfriend]
    #665801 - 06/06/02 11:42 AM (15 years, 25 days ago)

If you want to learn about cloning puerto ricans, go see star wars: episode 2.


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OfflineWorkmanV
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Re: CLONING in a hurry????? [Re: TheShroomHermit]
    #665886 - 06/06/02 12:35 PM (15 years, 25 days ago)

OMG!!!!!!! That is the funniest thing I have read in a long time! If I was drinking milk I am sure it would have come out my nose.

Workman wipes the tears from his eyes.


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Offlinephishyfriend
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Registered: 05/14/02
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Loc: new england
Last seen: 15 years, 11 days
Re: CLONING in a hurry????? [Re: TheShroomHermit]
    #666575 - 06/06/02 09:01 PM (15 years, 25 days ago)

i saw it but it didnt tell me how it only showed the outcome. i think that is the funniest thing ive heard in a long time....good one but seriously some one should help cause the only thing ive done since i posted this is laugh really hard


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OfflineAlpheratus
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Registered: 03/23/02
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Re: CLONING in a hurry????? [Re: phishyfriend]
    #666657 - 06/06/02 09:52 PM (15 years, 25 days ago)

ill give it a go....
ive heard this works well, but have yet to try it. im gonna try it in a few days.

This is for PFtek jars, but can be used in other teks. Take normal sterility precautions.
K, take a mushroom (preferably a big one, or one that grew fast) and cut the cap off, then take the stem of the mushroom and put it in a blender with about a cup of 1.5% hydrogen poroxid (w/ water). Turn on and off the blender quickly, use a pulse button if you have one on your blender. About 10 pulses should do the trick. Im pretty sure the water will be a blue slurry now, but im not sure. Take this mushroom slury and draw some up into a syringe and inject PF cakes like you would with spores. The colonization process should be faster cause you wont have to wait for germination.
correct me if im wrong about this tek.

hope that helped
good luck.


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Invisiblezeta
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Registered: 05/25/02
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Re: CLONING in a hurry????? [Re: TheShroomHermit]
    #666844 - 06/07/02 04:50 AM (15 years, 24 days ago)

you americans, you love to think everbody is american. temuera morrison is a new zealand maori


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OfflineMAIA
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Re: CLONING in a hurry????? [Re: zeta]
    #666857 - 06/07/02 05:17 AM (15 years, 24 days ago)

LOL !
... the bounty hunter. Storm troopers from NZ, never thought of that.

Peace,
MAIA


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Offlinephishyfriend
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Registered: 05/14/02
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Loc: new england
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Re: CLONING in a hurry????? [Re: Alpheratus]
    #667068 - 06/07/02 09:03 AM (15 years, 24 days ago)

thanks i will try that and good luck on yours. anyone else gotanother way or is this the way


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Offlinedubt
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Re: CLONING in a hurry????? [Re: Alpheratus]
    #668293 - 06/08/02 05:20 AM (15 years, 23 days ago)

>put it in a blender with about a cup of 1.5% hydrogen poroxid (w/ water).

do you mean one cup of 1.5% h202 straight from the bottle or a 1:10 ratio of 1.5% h202 and water. i'd like to try this and i don't want to fuck it up.
peace


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OfflineBaby_Hitler
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Re: CLONING in a hurry????? [Re: zeta]
    #668311 - 06/08/02 05:35 AM (15 years, 23 days ago)

Yeah but... The mushrooms are PR's.

Get it?



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Offlineggordon
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Re: CLONING in a hurry????? [Re: phishyfriend]
    #687869 - 06/19/02 09:54 AM (15 years, 12 days ago)

Dont want to sound like a dick but the first thing you should do is go out and buy the bible of mushroom cultivation "The Mushroom cultivator" by Paul Stamets. Great book and will help you with your sterile technque.

If you are using the blender then you have to make sure that you dont introduce contams. You do this by slicing the outer layer of the mushroom without touching it. As or the H2O2 (if you want to go that way) You should get the manual "growing mushrooms with H2O2." That way you will have a better idea of the volumes to use in your cloning. If you do use this tek then colonization will be impeded but your chances of contams greatly reduced.
It sounds like by the time you get this post your mushroom you want to clone will be long gone so you should do some reading and be ready for your next chance.


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