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 phishyfriend
newbie
Registered: 05/14/02
Posts: 34
Loc: new england
Last seen: 22 years, 7 months
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 CLONING in a hurry????? 
#665799 - 06/06/02 09:38 AM (22 years, 7 months ago) |
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Could someone tell me how to clone i have some puerto ricans(pardon spelling)and there pinning outside and i wanna bring them in. is there a way to do this fast? and is there a way to do this without a petri dish. could you give me exact measurments if needed please some one help. 
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TheShroomHermit
Divine Hermit of the Everything
Registered: 02/19/02
Posts: 7,575
Loc: border of Canada and Mexi...
Last seen: 1 year, 7 months
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 Re: CLONING in a hurry????? [Re: phishyfriend]
#665801 - 06/06/02 09:42 AM (22 years, 7 months ago) |
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If you want to learn about cloning puerto ricans, go see star wars: episode 2.
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,615
Loc: Oregon, USA
Last seen: 18 hours, 14 minutes
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OMG!!!!!!! That is the funniest thing I have read in a long time! If I was drinking milk I am sure it would have come out my nose.
Workman wipes the tears from his eyes.
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Research funded by the patrons of
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Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification  
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phishyfriend
newbie
Registered: 05/14/02
Posts: 34
Loc: new england
Last seen: 22 years, 7 months
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i saw it but it didnt tell me how it only showed the outcome. i think that is the funniest thing ive heard in a long time....good one but seriously some one should help cause the only thing ive done since i posted this is laugh really hard
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Alpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 18 years, 1 month
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Re: CLONING in a hurry????? [Re: phishyfriend]
#666657 - 06/06/02 07:52 PM (22 years, 7 months ago) |
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ill give it a go....
ive heard this works well, but have yet to try it. im gonna try it in a few days.
This is for PFtek jars, but can be used in other teks. Take normal sterility precautions.
K, take a mushroom (preferably a big one, or one that grew fast) and cut the cap off, then take the stem of the mushroom and put it in a blender with about a cup of 1.5% hydrogen poroxid (w/ water). Turn on and off the blender quickly, use a pulse button if you have one on your blender. About 10 pulses should do the trick. Im pretty sure the water will be a blue slurry now, but im not sure. Take this mushroom slury and draw some up into a syringe and inject PF cakes like you would with spores. The colonization process should be faster cause you wont have to wait for germination.
correct me if im wrong about this tek.
hope that helped 
good luck.
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blasted holes into night until she bled sunshine
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 zeta
Stranger
Registered: 05/24/02
Posts: 3,972
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you americans, you love to think everbody is american. temuera morrison is a new zealand maori
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 days, 23 hours
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Re: CLONING in a hurry????? [Re: zeta]
#666857 - 06/07/02 03:17 AM (22 years, 7 months ago) |
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LOL !
... the bounty hunter. Storm troopers from NZ, never thought of that.
Peace,
MAIA
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Spiritual being, living a human experience ... The Shroomery Mandala
 
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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phishyfriend
newbie
Registered: 05/14/02
Posts: 34
Loc: new england
Last seen: 22 years, 7 months
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Re: CLONING in a hurry????? [Re: Alpheratus]
#667068 - 06/07/02 07:03 AM (22 years, 7 months ago) |
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thanks i will try that and good luck on yours. anyone else gotanother way or is this the way
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dubt
Tao Teh Shroom
Registered: 05/26/02
Posts: 266
Loc: Florida
Last seen: 11 years, 2 months
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Re: CLONING in a hurry????? [Re: Alpheratus]
#668293 - 06/08/02 03:20 AM (22 years, 7 months ago) |
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>put it in a blender with about a cup of 1.5% hydrogen poroxid (w/ water).
do you mean one cup of 1.5% h202 straight from the bottle or a 1:10 ratio of 1.5% h202 and water. i'd like to try this and i don't want to fuck it up.
peace
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I'ts all a fucking lie! Everything written by me is fictional. I don't even exist!
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Baby_Hitler
Errorist
Registered: 03/06/02
Posts: 27,986
Loc: To the limit!
Last seen: 5 hours, 23 minutes
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Re: CLONING in a hurry????? [Re: zeta]
#668311 - 06/08/02 03:35 AM (22 years, 7 months ago) |
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Yeah but... The mushrooms are PR's.
Get it?
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Morality is just aesthetics, meatbags.
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ggordon
Stranger
Registered: 06/17/02
Posts: 17
Last seen: 19 years, 3 months
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Re: CLONING in a hurry????? [Re: phishyfriend]
#687869 - 06/19/02 07:54 AM (22 years, 7 months ago) |
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Dont want to sound like a dick but the first thing you should do is go out and buy the bible of mushroom cultivation "The Mushroom cultivator" by Paul Stamets. Great book and will help you with your sterile technque.
If you are using the blender then you have to make sure that you dont introduce contams. You do this by slicing the outer layer of the mushroom without touching it. As or the H2O2 (if you want to go that way) You should get the manual "growing mushrooms with H2O2." That way you will have a better idea of the volumes to use in your cloning. If you do use this tek then colonization will be impeded but your chances of contams greatly reduced.
It sounds like by the time you get this post your mushroom you want to clone will be long gone so you should do some reading and be ready for your next chance.
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