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OfflineCryogenicz
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microscope
    #6647343 - 03/07/07 11:50 PM (17 years, 2 months ago)



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OfflineRogerRabbitM
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Re: microscope [Re: Cryogenicz]
    #6648003 - 03/08/07 07:50 AM (17 years, 2 months ago)

It all depends on what you want it for. The lenses on the objectives look really small, so they won't take in much light.

I actually use my stereo microscope much more often than my light microscope. The light microscope is good if you want to identify bacteria or observe clamp connections in mycelium. If you want macro shots of gill structures or looking at the outside of objects, a stereo or zoom microscope is what you want. For a light microscope, look for an adjustable condenser so you can focus the light and the largest objectives you can find. By largest objectives, I mean the largest diameter, not the highest power. I rarely use 1000X. 400X gives much a clearer picture of clamp connections, so diameter of the lense becomes much more important then power.
RR


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OfflineWorkmanV
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Re: microscope [Re: Cryogenicz]
    #6648168 - 03/08/07 08:56 AM (17 years, 2 months ago)

That is the model I use. It is great for the price.


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OfflineCryogenicz
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Re: microscope [Re: Workman]
    #6648629 - 03/08/07 11:27 AM (17 years, 2 months ago)

I need something for detecting contaminates in LC, spores and aqueous solutions.


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Invisiblefastfred
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Re: microscope [Re: Cryogenicz]
    #6648707 - 03/08/07 11:41 AM (17 years, 2 months ago)

That looks like a pretty decent scope to me. They're standard DIN objectives, so you could always change them out. It doesn't list the NA specs for the objectives, but I would imagine that they are pretty standard numbers.


-FF

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OfflineCryogenicz
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Re: microscope [Re: fastfred]
    #6649306 - 03/08/07 02:19 PM (17 years, 2 months ago)

Can you tell if the stage moves x y and z?


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OfflineCryogenicz
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Re: microscope [Re: Cryogenicz]
    #6649316 - 03/08/07 02:21 PM (17 years, 2 months ago)



--------------------
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OfflineRogerRabbitM
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Re: microscope [Re: Cryogenicz]
    #6651089 - 03/08/07 10:24 PM (17 years, 2 months ago)

The last one for sure has x,y and z adjustments. It's the shaft that hangs down from the stage near the base with two knobs on it. One moves the stage left and right, the other front and back. The focus knob moves it up and down.
RR


--------------------
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OfflineCryogenicz
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Re: microscope [Re: RogerRabbit]
    #6651302 - 03/08/07 11:30 PM (17 years, 2 months ago)

RR - Which one of the AMscopes on ebay would you get? Im thinking the last link I sent is a keeper.. what do you think?


--------------------
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OfflineRogerRabbitM
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Re: microscope [Re: Cryogenicz]
    #6651361 - 03/08/07 11:47 PM (17 years, 2 months ago)

I'm not experienced with any of those, so I'd go with what workman said. I got ripped off by ebay/paypal five years ago and I've never forgiven them, so I refuse to shop there.

I have a couple of scopes in that genre that sit on my shelf unused. I bought a medical lab scope that is so much clearer, with larger objectives and eyepieces and a light that can be focused, so it's the one I use. Definitely get a trinocular model with a ccd tube adapter so you can take pictures.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlineshroomsynergy
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Re: microscope [Re: RogerRabbit]
    #6651717 - 03/09/07 02:23 AM (17 years, 2 months ago)

thats about the same that i have, and i can see the contams pretty easy. i tend to go too far in, and dont know what the fuck i'm looking at, but if i stay between 300-700 i seem to be able to tell better whats going on!\

i'm still new at this too, but well, thats what i noticed so far. lol


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OfflineCryogenicz
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Re: microscope [Re: shroomsynergy]
    #6654333 - 03/09/07 09:44 PM (17 years, 2 months ago)

ok, cool... Thank you for the information.


-Graham


--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
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orders@mycopath.com
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OfflineCryogenicz
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Re: microscope [Re: Cryogenicz]
    #6663586 - 03/13/07 01:16 AM (17 years, 2 months ago)



--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.

www.FungiForum.com

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OfflineRogerRabbitM
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Re: microscope [Re: Cryogenicz]
    #6664034 - 03/13/07 09:07 AM (17 years, 2 months ago)

Get ready to be addicted to the miniature world for a while when it comes. There's a whole new universe you'll see and it's a facinating one. Did you get a camera too? The best microscope cameras are the ccd tube mounts, but they're a bit spendy. A cheaper alternative is the ones that replace an eyepiece, but they don't have the clarity or resolution, but still get the job done. Have fun!
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineCryogenicz
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Re: microscope [Re: RogerRabbit]
    #6664316 - 03/13/07 11:03 AM (17 years, 2 months ago)

I didnt get a camera, I cant afford it.

I really just need one now so I can verify that my cultures are contaminate free.

The camera is next on the list, but I dont see how I "have" to have it now.

-Graham


--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.

www.FungiForum.com

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OfflineRogerRabbitM
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Re: microscope [Re: Cryogenicz]
    #6664352 - 03/13/07 11:19 AM (17 years, 2 months ago)

If you make your product in large batches, it's always best to test on grains or agar a sample from each batch. Sometimes the single drop of liquid you put on a slide will be clean, but there could still be bacteria or mold spores in the rest of it. Just a heads up.

Which reminds me. Be sure to order a box of slides and cover slips.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineWorkmanV
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Re: microscope [Re: RogerRabbit]
    #6664390 - 03/13/07 11:34 AM (17 years, 2 months ago)

Yeah, RR is right. Visually checking for contamination with a microscope isn't going to work very well. Its fine for checking the identity of contaminates you can already see like a spot of mold or bacteria on a petri dish. I guess it might be helpful to look at questionable liquid cultures that have large amounts of bacteria or yeast, but a simple drop test on agar would be more definative.

Enjoy the scope, they are fun!


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Invisiblefastfred
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Re: microscope [Re: Workman]
    #6666325 - 03/13/07 08:01 PM (17 years, 2 months ago)

I think it's pretty useful to have a microscope for checking contamination. With liquid cultures possibly contaminated with yeast or bacteria it's quite easy to check for that under the scope. Yeast and bacteria replicate very quickly and by the time the culture is ready or you notice any suspicious signs you will be able to spot the contamination in every drop you throw on a slide. Checking cultures this way saves the cost of a petri and the time it takes to grow it out.

You can't verify a culture is free from a handful of stray spores this way, so it's not the most effective way to find fungal spore contamination. OTOH there are times when you spot a white fungal spot on a petri and can't visually tell what it is right away. Using a scope you can look at the morphology and figure out if it's contamination or not right away. So using a scope to check petris also helps save time and the danger of growing up contaminants to the point where they produce spores.

A couple tricks for being able to look directly at petris under your scope is to either get an ULWD (ultra long working distance) objective or to pour your petris very full so that the distance looking through the dish to the surface is reduced. It's very fun to be able to look directly at a growing dish without disturbing it or the hassle of preparing slides. Some scopes have mechanical stages that will hold a whole petri, with others it's slightly more difficult but still very useful. The one you got looks like the clip is big enough to hold the petri in it, which makes it very easy to move around. Using parafilm or some other sort of wrap around the edge of your dishes is also helpful by keeping them together and sealed while working with them.


-FF

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OfflineCryogenicz
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Re: microscope [Re: fastfred]
    #6666555 - 03/13/07 09:02 PM (17 years, 2 months ago)

Thank you for all of your information.  You have been very helpfull.

Thankyou RR, FF, and Workman :smile:

-Graham


--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.

www.FungiForum.com

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OfflineRogerRabbitM
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Re: microscope [Re: Cryogenicz]
    #6666746 - 03/13/07 09:46 PM (17 years, 2 months ago)

I can put a closed petri in my cheap microscope, but my better lab one doesn't have the working distance to be that far away, even if I fill the dish up. In fact, with the stage all the way down, I can't even fit a petri in under anything but the 4X objective. It's barely above a cover slip on a slide when the lenses are in focus at anything over the 10X objective. That's the only reason I don't get rid of the cheapie.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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