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Offlineillahee
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DET Shroom possiblity
    #6616120 - 02/27/07 10:06 AM (17 years, 2 months ago)

So I'm posting this just to get a feedback from the general consensus of the forum as to whether I'm a dumb ass or not. Now I am aware of the search bar, but couldn't find the article I was looking for...

Does anyone remember from a long ass time ago (2 years or so) someone posting a grow log of some cubensis they grew? Oh shit illahee! I remember!!!! lol, jk. It was a special grow log, the guy used top ramen noodles as his substrate. His mushrooms were beautiful but turned out to be a dud.....the ramen just didn't have the nutrients to produce psilocybin.

Well everyone knows (or everyone should know) that the addition of dimethyltryptamine (DMT) into a mushrooms substrate gets turned into DET by the natural hydroxylation process of our friends the mushrooms.


So......

If such a substrate as ramen was used as to give conditions where no natural alkaloids were produced, but the substrate was impregnated with DMT, would you get DET shrooms?

I think this would be pretty fucking cool. RC's at home. Oh yeah, also..... If anyone has info on the separation of baeocystin with a chroma column could they pm me plz.

Thanks all.

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Invisiblefastfred
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Re: DET Shroom possiblity [Re: illahee]
    #6623027 - 03/01/07 02:28 AM (17 years, 2 months ago)

I don't see why it wouldn't work. But you'd need quite a bit of DMT, and DET is less than half as potent as psilocybin. It's also less potent than DMT itself, so I don't see much advantage unless you're simply trying to convert DMT into something that's orally active.

It would be an interesting experiment, but I don't see much practical use for doing it.

EDIT: I should have noticed that you said DET. You'd never get that, but possibly 4-HO-DMT instead.


-FF

Edited by fastfred (03/01/07 11:04 PM)

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OfflineRogerRabbitM
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Re: DET Shroom possiblity [Re: fastfred]
    #6623565 - 03/01/07 08:24 AM (17 years, 2 months ago)

Quote:

Well everyone knows (or everyone should know) that the addition of dimethyltryptamine (DMT) into a mushrooms substrate gets turned into DET by the natural hydroxylation process of our friends the mushrooms.





Prove it. I've seen zero evidence of this. In fact, I've seen zero evidence that substrate plays a roll in potency, provided the basic requirements of the mycelium are met. My isolates produce equal potency regardless of substrate, therefore I say potency is strain related.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinekong
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Re: DET Shroom possiblity [Re: RogerRabbit]
    #6624547 - 03/01/07 02:01 PM (17 years, 2 months ago)

The "hydroxylation process" does exactly that. It will add the "hydroxy" to the 4 position on the dmt. Hence, 4-ho-dmt. It will not change dmt to det.

Being in possension of DMT is good enough in it's self. Possension of DET is just as good as it is much more rare than DMT. You could put DET into a mushroom substrate and get 4-ho-det out, as well as 4-ho-dmt in most cases. Putting DET into a mushroom substrate would be a waste however, because there are many suppliers for 4-ho-det, and almost no sources for DET itself, (it's illegal, and quite good:)).

Heres something interesting http://www.shroomery.org/9040/Tryptamine-Cubensis

I think using mimosa just to make mushrooms more potent is a waste. I believe just about anyone who has ever TRIED dmt would aggree. On the other hand, trypamine is easy to make, and yeilds results that are just as good or better than the DMT laden mimosa, (which should be used to make dmt anyway).

Did I mention tryptamine is easy to make?

Tryptophan is CHEAP AND EASY TO GET. Its one of the essential amino acids the body cannot produce on its own, and as such is widely availible as a suppliment.

Adding tryptamine WORKS, and works well. Get ready to be run over by two gram doses.

***sorry to hijack the thread, but it was based on make-believe chemistry anyway:), and I keep seeing people using precious mimosa, when a easily modified supplement will work better and cheaper. Shit, go overboard...use 1/2g tryptamine a Quart like I do. Eat two grams, and SMOKE your DMT:) Just make sure anyone else understands what they've got***

Spearmint Oil Catalyzed Decarboxylation of Tryptophan

by "Student"

A mixture of 75 mL of turpentine (1), 7.14 grams of L-tryptophan (2), and 15 drops (0.25 grams; 0.3 mL) of spearmint oil (3) were placed in a 250 mL Erlenmeyer flask. A water cooled reflux condenser (4) was attached to the flask by a rubber stopper (5). The mixture in the flask was boiled (6)fast enough that there was at least one drop returning to the flask from the condenser every second. The mixture became transparent in four hours and heating was turned off after another 30 minutes. There was a little yellow solid on the side of the flask above the liquid. After sitting overnight there was a clump of yellow crystals in the corner of the flask and solidified dark oil across the bottom. The flask was refrigerated for the day and the orangish mother liquor was poured off.

The impure tryptamine was purified as follows (7). To the flask were added 150 mL of 5% distilled household vinegar along with 5 mL of chloroform (8) and the flask was briskly swirled until all solid was gone and there was only a little dark brown oil not dissolved in the yellow suspension. The hazy yellow liquid (pH 5-6) upper layer was filtered through a plug of cotton. The small amount of dark brown lower organic layer was extracted with another 10 mL of vinegar, and the resulting upper layer was filtered through the cotton plug. To the combined filtrates were added 5 mL of chloroform and enough sodium bicarbonate (10.58 g) in portions so that further addition caused very little foaming. The flask was swirled thoroughly and the hazy yellow aqueous upper layer was filtered through a fresh plug of cotton. The filtrate was cooled in the freezer for 15 minutes, basified with 12 mL of 25% sodium hydroxide solution, and set back in the freezer for 30 minutes. The solid was dislodged from the sides with a metal scoop and the mixture was filtered through filter paper (9). The flask and crystals were rinsed with 100 mL of ice cold household ammonia in portions (10). The filter paper was pressed between paper towels until damp and set aside to dry. The light yellow crystals weighed 3.64 grams (65% yield).

The turpentine mother liquor from the last reaction, still containing spearmint oil and some tryptamine, was used directly to decarboxylate 7.23 grams of L-tryptophan. This time the reaction took seven hours to become transparent, so apparently some of the catalyst was consumed during the first reaction. This time both the turpentine and the solid product were extracted with vinegar as above, and brought through the same purification process, to give 5.21 grams (92% yield) of light yellow crystals. The combined yield of tryptamine for the last two reactions is 79%. The solid melted at 117-118.5°C (Merck 118°C) and had one tan spot (Rf ~0.1 - 0.2) on silica TLC, eluting with methanol containing ~50 mg of ammonium carbonate.

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Offlineillahee
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Re: DET Shroom possiblity [Re: kong]
    #6624992 - 03/01/07 04:24 PM (17 years, 2 months ago)

thanks for the replies.

Rabbit, do you remember the top ramen thread, grow log? I know it was on the shroomery. no problem on the thread hijack, I was stoned as hell. Thanks guys.

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OfflineJorsher
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Re: DET Shroom possiblity [Re: illahee]
    #6626473 - 03/01/07 10:59 PM (17 years, 2 months ago)

That looks interesting Kong...


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OfflineRogerRabbitM
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Re: DET Shroom possiblity [Re: Jorsher]
    #6626696 - 03/02/07 12:21 AM (17 years, 2 months ago)

I do remember the ramen noodle thread, but I believe it was on a different site, not this one. I don't think it worked out all that well, or he would have kept it up. Pasta is pretty starchy.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineKidgardFromSRQ
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Re: DET Shroom possiblity [Re: RogerRabbit]
    #6904393 - 05/11/07 10:41 PM (17 years, 11 days ago)

Quote:

RogerRabbit said:
Quote:

Well everyone knows (or everyone should know) that the addition of dimethyltryptamine (DMT) into a mushrooms substrate gets turned into DET by the natural hydroxylation process of our friends the mushrooms.







Prove it. I've seen zero evidence of this. In fact, I've seen zero evidence that substrate plays a roll in potency, provided the basic requirements of the mycelium are met. My isolates produce equal potency regardless of substrate, therefore I say potency is strain related.
RR




I know mushrooms love nitrogen, try growing it on pure blood meal. I'm pretty sure the more nitrogen the more the fruiting body creates a stronger immune system, and thus has greater psilocybin. I could be wrong though. But I'd like to hear an antithesis.


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OfflineRogerRabbitM
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Re: DET Shroom possiblity [Re: KidgardFromSRQ]
    #6904598 - 05/11/07 11:58 PM (17 years, 11 days ago)

I'd like to see anything grow on pure blood meal. What makes you think nitrogen boosts psilocybin? (other than you read it somewhere)
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Invisiblefastfred
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Re: DET Shroom possiblity [Re: RogerRabbit]
    #6904967 - 05/12/07 03:26 AM (17 years, 11 days ago)

You've never seen the famed "blood meal boomer" RR?

It's incredibly potent, but can sometimes develop the deadly photocybin if not grown and prepared properly.


-FF

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OfflineKidgardFromSRQ
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Re: DET Shroom possiblity [Re: RogerRabbit]
    #6909869 - 05/13/07 01:09 PM (17 years, 10 days ago)

Quote:

RogerRabbit said:
I'd like to see anything grow on pure blood meal. What makes you think nitrogen boosts psilocybin? (other than you read it somewhere)
RR




Well, my theories are mostly from what I've read. I'm no expert at all in growing shrooms or knowing about growing shrooms. I find it interesting and you could probably say more from field experience and most likely from a mycologists perspective. You've done some cool shit, I've seen it.

From an evolutionary stand point though, I've always looked at things in terms of survival of the fittest. I believe that the more nutrients readily available, the more thing will use those nutrients to build its defense system (which partly is psilocybin, along with metabolism, size growth, etc). But it's all survival tacticts as far as I'm concerned, genetically speaking of course.

Is this theory/hypothesis incorrect?


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