Home | Community | Message Board


Mycotrop.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Edibles   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Original Sensible Seeds High THC Strains   PhytoExtractum Kratom Powder for Sale   Amazon ½ Pint Jars, Agar, Magnetic Stirrer, Petri Dish, Portable Greenhouse

Jump to first unread post. Pages: 1
InvisibleHelltick
Player
 User Gallery

Registered: 10/30/05
Posts: 684
Loc: Deep...
Is this the right Agar?
    #6609009 - 02/25/07 05:05 PM (14 years, 2 months ago)



If so...could someone please point me to the best Tek to prepare this stuff in order to bring back a dried specimen?

Thx.


--------------------
  My Agar Tek. 
  My Greenhouse Tek. 

"It's not good when it's done, it's done when it's good"


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,200
Loc: upstate NY, USA
Last seen: 2 days, 19 hours
Trusted Cultivator
Re: Is this the right Agar? [Re: Helltick]
    #6609027 - 02/25/07 05:11 PM (14 years, 2 months ago)

That's the stuff. Bear in mind, it's just the jelling agent.

I've had the best luck bringing back dried fruits by placing in a jar of sterilized distilled water with zero nutrients added. Be sure to use a filter in the lid for fresh air. It will also help if you have a magnetic stirrer. It takes about six to eight weeks, and you'll see mycelium growth into the distilled water. At this time, transfer some of the mycelium to your agar mix to isolate away from any contaminants that may be growing along with it.

I use 20 grams agar agar, 20 grams malt dextrose, and 2 grams brewers yeast per liter of water.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleHelltick
Player
 User Gallery

Registered: 10/30/05
Posts: 684
Loc: Deep...
Re: Is this the right Agar? [Re: RogerRabbit]
    #6609326 - 02/25/07 06:39 PM (14 years, 2 months ago)

Thanks RR,

What about a fresh specimen? how long does that take?
Or even spores that have gathered in my greenhouse?

BTW...I found this at a local grocery store in the Asian/Oriental Isle.
Don't ask the staff, cuz they'll just look at You like WTF and say no we don't have it.

Look your self for "Agar Agar" it's $1.35 per package.

Thx.


--------------------
  My Agar Tek. 
  My Greenhouse Tek. 

"It's not good when it's done, it's done when it's good"


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleHelltick
Player
 User Gallery

Registered: 10/30/05
Posts: 684
Loc: Deep...
Re: Is this the right Agar? [Re: Helltick]
    #6609446 - 02/25/07 07:13 PM (14 years, 2 months ago)

I've also read you can substitute table sugar for dextrose...is this true?

I will be using potato water also.

Thx.


--------------------
  My Agar Tek. 
  My Greenhouse Tek. 

"It's not good when it's done, it's done when it's good"


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinechucklehead
GeneticThrowback toNeanderthal
Male User Gallery

Registered: 02/17/07
Posts: 1,338
Loc: Chucky Cheese
Last seen: 1 year, 10 months
Re: Is this the right Agar? [Re: Helltick]
    #6609522 - 02/25/07 07:31 PM (14 years, 2 months ago)

Are you kidding me?!?! I paid $40 for my pre-mixed Agar/Malt after shipping. DOH! I've said it before and I'll keep on saying it. Ignorance and laziness are expensive.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Is this the right Agar? [Re: Helltick]
    #6609704 - 02/25/07 08:22 PM (14 years, 2 months ago)

I use this one...

-PDYA-

500ml water
5 grams of instant potato flakes
9 grams agar agar
10 ml honey
1 gram of Nutritional Yeast(optional)


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleHelltick
Player
 User Gallery

Registered: 10/30/05
Posts: 684
Loc: Deep...
Re: Is this the right Agar? [Re: Roadkill]
    #6609836 - 02/25/07 08:52 PM (14 years, 2 months ago)

Thanks guys,

I went shopping and being Sunday all brew stores were closed.
I have a couple questions I really need answered...

-Can I substitute dextrose with normal table sugar?
-Do I need brewers yeast or does normal bread yeast work?
-Can I use these...



...as petri dishes?

Thx a bunch.


--------------------
  My Agar Tek. 
  My Greenhouse Tek. 

"It's not good when it's done, it's done when it's good"


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,200
Loc: upstate NY, USA
Last seen: 2 days, 19 hours
Trusted Cultivator
Re: Is this the right Agar? [Re: Roadkill]
    #6609847 - 02/25/07 08:54 PM (14 years, 2 months ago)

To tell ya the truth, forty bucks isn't that bad for a pound of premixed agar. It's enough for about 500 petri dishes so it goes a long way. I've mixed it myself, and I've bought it premixed. I prefer the latter, but that's because I have too many other projects going, so it helps to let somebody else do part of the work.

When you go to the oriental food store, ask for 'auger auger' because that's how they say it. If you pronounce it aager, they will just look at you funny.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleHelltick
Player
 User Gallery

Registered: 10/30/05
Posts: 684
Loc: Deep...
Re: Is this the right Agar? [Re: RogerRabbit]
    #6609875 - 02/25/07 09:00 PM (14 years, 2 months ago)

Ok...but I already have this stuff and was local.

Can I use the above things as petri dishes?


--------------------
  My Agar Tek. 
  My Greenhouse Tek. 

"It's not good when it's done, it's done when it's good"


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,200
Loc: upstate NY, USA
Last seen: 2 days, 19 hours
Trusted Cultivator
Re: Is this the right Agar? [Re: RogerRabbit]
    #6609884 - 02/25/07 09:02 PM (14 years, 2 months ago)

Those look a bit small to be of much use. I also doubt you could PC them. You want petri dishes that are about the diameter of your beer can.

I recommend the plastic, presterilized dishes. They're in a sleeve of 20. Don't open the sleeve until you're ready to pour the agar, and open it inside the glovebox. After pouring, put the sleeve back over the stacked dishes. It will help reduce condensation and will protect the unsealed dishes from contaminants as they cool.

Let your agar cool 'almost' to room temperature before pouring. I estimate my agar is about 100F to 110F when I pour. You should need no protection from the heat of the bottle for your pouring hand. This will cut down drastically on condensation on the lids.

It's cheapest to buy a box of 500 dishes at a time. It's amazing how fast you go through them once you get to isolating strains.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleHelltick
Player
 User Gallery

Registered: 10/30/05
Posts: 684
Loc: Deep...
Re: Is this the right Agar? [Re: RogerRabbit]
    #6610283 - 02/25/07 10:33 PM (14 years, 2 months ago)

Whats the difference between Nutritional Yeast and normal bread yeast you buy at the grocery store?


--------------------
  My Agar Tek. 
  My Greenhouse Tek. 

"It's not good when it's done, it's done when it's good"


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleHelltick
Player
 User Gallery

Registered: 10/30/05
Posts: 684
Loc: Deep...
Re: Is this the right Agar? [Re: Helltick]
    #6610652 - 02/25/07 11:35 PM (14 years, 2 months ago)

Well, guess I'll just STFU and use the search function...but it's just so vast when it comes to agar prep.

I have a very good idea...just a few missing links.
If anyone is kind enough to share there experience with agar...that would certainly be awesome.

But I'll tell ya...when I figure this shit out, I'm gonna write the best
Pictorial agar Tek ever!

Thx.


--------------------
  My Agar Tek. 
  My Greenhouse Tek. 

"It's not good when it's done, it's done when it's good"


Edited by Helltick (02/25/07 11:48 PM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleBlutjager
Inhuman
Male

Registered: 06/12/06
Posts: 9,220
Re: Is this the right Agar? [Re: Helltick]
    #6611293 - 02/26/07 03:01 AM (14 years, 2 months ago)

Quote:

Helltick said:
When I figure this shit out, I'm gonna write the best
Pictorial agar Tek ever!




If you spend half as much effort on it as you did with your kick ass greenhouse tek it will really be an asset to many members of the shroomery including myself and I look forward to seeing it


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Bridgetown Botanicals CBD Edibles   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Original Sensible Seeds High THC Strains   PhytoExtractum Kratom Powder for Sale   Amazon ½ Pint Jars, Agar, Magnetic Stirrer, Petri Dish, Portable Greenhouse

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Malt and agar dosent react after 12 days !!! TheMessenger 889 2 05/05/03 03:02 AM
by Prisoner#1
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 16,559 38 05/11/01 01:08 PM
by MAIA
* some questions about agar ar393 887 3 06/11/01 05:04 PM
by Anno
* Agar question sill_o_cin 3,079 4 11/21/01 07:23 PM
by sill_o_cin
* Re: agar questions Anonymous 1,094 6 05/30/00 05:07 PM
by Anonymous
* Agar Anonymous 1,302 8 04/15/04 12:15 PM
by Bi0TeK
* Question about agar nd595 3,316 18 03/10/04 06:35 PM
by Anonymous
* dish sterilization dog 1,064 7 01/31/03 02:34 PM
by deanofmean

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
1,177 topic views. 13 members, 82 guests and 48 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.009 seconds on 16 queries.