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Offlinesketchydelux
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Re: walls and lid around front of flow hood? [Re: fastfred]
    #6617803 - 02/27/07 06:56 PM (17 years, 2 months ago)

Quote:

fastfred said:






-FF




thats a cool flowhood but jesus that dood has to pull his socks up for equilibrium


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OfflineCryogenicz
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Re: walls and lid around front of flow hood? [Re: sketchydelux]
    #6618301 - 02/27/07 09:02 PM (17 years, 2 months ago)

Gotta love flowhoods


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Invisiblefastfred
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Re: walls and lid around front of flow hood? [Re: caricapapaya]
    #6623013 - 03/01/07 02:12 AM (17 years, 2 months ago)

Quote:

caricapapaya said:
The vertical flow hood shown on that link is a biological safety hood, or type of containment hood. They provide inward airflow to prtoect the user, filtered internal air to protect the project, and filtered exhaust so as not to exhaust dangerous stuff into the airspace in the lab,





Nope, it's a flow hood. Some flow hoods look like fume hoods because they're designed almost identical a lot of times. I've never even used the type of hood you're talking about. Way to expensive. I don't think I've ever even actually seen one.

You don't work with dangerous biologicals in any sort of hood. You do it at your lab bench or in a glove box if it's really dangerous. It's not safe to work with them in a flow hood because it would be blowing it back at you and most fume hoods don't have good enough filtration, if any, on the exhaust. Organisms that produce dangerous airborne particles are pretty rare anyway, about the only one I can think of is anthrax.


-FF

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Invisiblephalcon005

Registered: 12/21/05
Posts: 217
Re: walls and lid around front of flow hood? [Re: fastfred]
    #6623783 - 03/01/07 09:28 AM (17 years, 2 months ago)

Quote:

fastfred said:
Quote:

caricapapaya said:
The vertical flow hood shown on that link is a biological safety hood, or type of containment hood. They provide inward airflow to prtoect the user, filtered internal air to protect the project, and filtered exhaust so as not to exhaust dangerous stuff into the airspace in the lab,





Nope, it's a flow hood. Some flow hoods look like fume hoods because they're designed almost identical a lot of times. I've never even used the type of hood you're talking about. Way to expensive. I don't think I've ever even actually seen one.

You don't work with dangerous biologicals in any sort of hood. You do it at your lab bench or in a glove box if it's really dangerous. It's not safe to work with them in a flow hood because it would be blowing it back at you and most fume hoods don't have good enough filtration, if any, on the exhaust. Organisms that produce dangerous airborne particles are pretty rare anyway, about the only one I can think of is anthrax.


-FF



Caricapapaya had it right, there are vertical flow hoods designed for biological agents and that is how they are all processed in pathogenic labs. For my day job I work in a pathogenic lab that processes pathogenic bacteria, in particular there is a hood 15 feet from me now that I routinely process plague and tularemia samples in. Tularemia is one of the nastiest to work with in a lab, one only has to inhale 5-10 organisms to get pneumonic tularemia which will land you in the hospital for a few weeks. I wish I could get a picture of my hood, but here is something similar


And to try to clarify the air flow

Bleh, that image didn't take so well so here is another

All incoming air comes from the front but is sucked into the system before it gets to your work area. For exhaust, they either have duct work to vent outside or simply recirculate into the room. The one I work with simply filters the air and kicks it back into the room, as most of them do at our facility.

The lab I work with has about 40 of these is our main building. I am tickled to see discussion on using them for mycological purposes because I'm working with maintanence to get my hood when it is decommissioned soon. I would never buy something this elaborate for mycological purposes, but I think it would work nicely. I suppose if you worked with a horizontal flow hood it would take time to get used to the vertical flow and avoiding crossing over your sterile work, but at work we use these for sterile culture work all the time with great success.

Edited by phalcon005 (03/01/07 09:35 AM)

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Offlinecaricapapaya
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Re: walls and lid around front of flow hood? [Re: phalcon005]
    #6624004 - 03/01/07 10:47 AM (17 years, 2 months ago)

Yeah, Phalcon, there are many types of sterile flow hoods, containment hoods being one class. I am sitting in a lab about 20 ft from one right now that I use every day for sterile work.

Ive used vertical and horizontal types with success before.

That would be a great find to get one for free! although I would want to be able to really give it a thourough cleaning if its been used for freaky stuff...


carica

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Invisiblefastfred
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Re: walls and lid around front of flow hood? [Re: caricapapaya]
    #6626455 - 03/01/07 10:54 PM (17 years, 2 months ago)

I know that there are containment hoods, but the one in my post is just a flow hood. Note that there is no vent by the front lip or complicated flow system mounted on it.

I realize that containment hoods exist, I've just never had cause to use one. The worst I've ever worked with is weakened Y. Pestis. All the labs I've been in use just flow hoods and fume hoods. Exposure rarely happens because of bacteria becoming airborne, so there really isn't all that much reason to use a containment hood in most cases.


-FF

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Offlinecaricapapaya
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Re: walls and lid around front of flow hood? [Re: fastfred]
    #6627912 - 03/02/07 12:24 PM (17 years, 2 months ago)

FF, sorry for the confusion, I realize now what you are talkking about,.

I was referring to the link posted by Arp:

http://www.globalrph.com/aseptic.htm

one of the pictures there is the hood I was talking azbout.

I agree with you, especially for mushroom work, theres no real need for something like that.

good growing!
carica

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