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JoelLoveland5
Giant Reptile


Registered: 11/11/06
Posts: 222
Last seen: 16 years, 10 months
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Sterile Syringe for LC
#6599356 - 02/22/07 08:15 PM (16 years, 11 months ago) |
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When Using an LC to inoculate I was wondering what the common practice was on sterilization of the syringe.
My syringes come new in sealed plastic. Because of this would I only need to flame the needle before I suck up some LC with it and then again before I inject. Or would a more strict regiment need to be implemented.
Thanks for your thoughts guys.
Later
-JL5000
-------------------- Newb Friendly Tek for The Lazy by "Hydro420" http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5278987/an/0/page/0 Great Newb Friendly Cloning Tek by "Creamcorn" http://www.shroomery.org/forums/showflat.php/Number/5874305#5874305 One Goal, One Passion - Joel Loveland "Dont Be Wise In Your Own Opinions"
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figment
Apothecary


Registered: 01/04/06
Posts: 522
Loc: Quidity
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you're correct. but make sure you cool the needle with an an alcohol swab before you inject...that will also insure the outside of the needle is sterile.
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JoelLoveland5
Giant Reptile


Registered: 11/11/06
Posts: 222
Last seen: 16 years, 10 months
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Re: Sterile Syringe for LC [Re: figment]
#6599403 - 02/22/07 08:27 PM (16 years, 11 months ago) |
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So no steam sterilization or anything?
Later
-JL5000
-------------------- Newb Friendly Tek for The Lazy by "Hydro420" http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5278987/an/0/page/0 Great Newb Friendly Cloning Tek by "Creamcorn" http://www.shroomery.org/forums/showflat.php/Number/5874305#5874305 One Goal, One Passion - Joel Loveland "Dont Be Wise In Your Own Opinions"
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JonnyOneNut
Sponge


Registered: 11/13/06
Posts: 303
Loc: Massachusetts
Last seen: 7 years, 11 months
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You could boil the syringe in some hot water first then suck up some boiling water a few times. Followed by a little bit of alcohol, then some more boiling water (a few times) to clean it out. Then flame and cool with alcohol pad or alcohol soaked paper towel.
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BABY BOTTLE LC TEK Fix it or accept it, but do not stress about it.
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rogermarklar
TranscendentalJournier


Registered: 12/23/06
Posts: 62
Last seen: 16 years, 10 months
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Re: Sterile Syringe for LC [Re: JonnyOneNut]
#6599565 - 02/22/07 09:08 PM (16 years, 11 months ago) |
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This is how I did it, and i have had a 100% success rate this way. I know you have brand new steril syringes.. but i will include sterilizing them regardless just for anyone that doesnt have them new in plastic.
Fill your syringes w/ water and wrap them in tinfoil. I wrap them several different layers. Also wrap the needle that attaches to the syringe in foil. Pop them in a PC and cook them for 90 minutes.
In a clean area, or w/ a glove box, have a couple cotton balls soaked in alcohol on a clean plate and a lighter.
throw out the water that was in the syringe when you PC'd it. Assemble the syringe w/ the needle. Use a lighter and get the needle red hot. make sure you hold the needle high above the tip of the flame or else you will get black shit all over it. use one of the alcohol cotton balls and wipe off the needle. this also allows the needle to cool. pop the sucker into your LC and fill the syringe. when you take the needle out of the LC, do the same thing. Flame it, and wipe it.
Before sticking your needle into your jars, take a different alcohol soaked cotton ball and wipe downt hte entire surface of the tyvek. then inoculate it with a few CC's.. between each jar, rewipe w/ alcohol as well as that jars tyvek cover.
I only flame before and after going into my LC bottle...
hope that helps!
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JoelLoveland5
Giant Reptile


Registered: 11/11/06
Posts: 222
Last seen: 16 years, 10 months
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Re: Sterile Syringe for LC [Re: rogermarklar]
#6599619 - 02/22/07 09:19 PM (16 years, 11 months ago) |
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Thanks for the info guys
Later
-JL5000
-------------------- Newb Friendly Tek for The Lazy by "Hydro420" http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5278987/an/0/page/0 Great Newb Friendly Cloning Tek by "Creamcorn" http://www.shroomery.org/forums/showflat.php/Number/5874305#5874305 One Goal, One Passion - Joel Loveland "Dont Be Wise In Your Own Opinions"
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Hypercube
80 SRM



Registered: 12/18/05
Posts: 814
Last seen: 11 years, 21 days
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IMO, 90 mins in a PC is way overkill for a syringe. 20 mins works for me, I just load them in an autoclave bag, rolled up w/ a rubber band on it. Also, alcohol + flame in a glove box is not the best idea.
If you have new syringes/needles, no need to flame anything and just wipe the needle with alc swab after loading it with LC in a g/b. I never flame sterilize needles anyways, but that's just me.
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nugjug
Wanderer

Registered: 11/07/05
Posts: 703
Last seen: 5 years, 11 months
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Re: Sterile Syringe for LC [Re: Hypercube]
#6600527 - 02/23/07 01:10 AM (16 years, 11 months ago) |
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Quote:
Hypercube said: IMO, 90 mins in a PC is way overkill for a syringe. 20 mins works for me, I just load them in an autoclave bag, rolled up w/ a rubber band on it. Also, alcohol + flame in a glove box is not the best idea.
If you have new syringes/needles, no need to flame anything and just wipe the needle with alc swab after loading it with LC in a g/b. I never flame sterilize needles anyways, but that's just me.

Some syringes can't hold up to that high of heat for that long. I had some cheaper ones that I ordered off the internet and after 60 minutes the rubber gasket tore. I also don't use a flame anymore after the time I spilt rubbing alcohol in my glove box and then subsequently lit it on fire. Now I just use agar's advice of the injection condom using project foam soaked in lysol to cover the syringe needle. When you inject it compresses and when you pull the needle out it decompresses keeping the needle covered at all times.
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ItsaMeMario
Stranger
Registered: 01/01/07
Posts: 359
Last seen: 5 years, 8 months
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Re: Sterile Syringe for LC [Re: nugjug]
#6600711 - 02/23/07 02:19 AM (16 years, 11 months ago) |
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can you just suck up rubbing alchohol with the syringe couple of times? thats what i did last time with my LC and it contaminated. but logically it seems like it should work tho...
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: Sterile Syringe for LC [Re: ItsaMeMario]
#6601201 - 02/23/07 08:22 AM (16 years, 11 months ago) |
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Quote:
ItsaMeMario said: can you just suck up rubbing alchohol with the syringe couple of times? thats what i did last time with my LC and it contaminated. but logically it seems like it should work tho...
Alcohol is not a sterilizer. It's a disinfectant, but it cannot sterilize.
I think some of you are going a little overboard with your sterilization procedures, that is unless you've thrown your syringes into a mud bog or the trash or something.
All I have ever done with spore syringes or LC is to bring some water to a boil in a pot on the stove. I aspirate the boiling water into the syringe, spray the water out of the syringe into the sink, and repeat 2-3 times, making sure to dip as much of the needle into the hot water as possible. On the last go, I don't spray the water out, I just keep the syringes full of hot water and let it sit on the counter for 30 minutes.
Then I empty the syringes, alcohol swab the needle and the injection point, and start sucking up LC.
All my personal results. I haven't yet had a contaminated syringe in 3 years using this technique, so I haven't had a need to consider lengthier methods yet.
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