Clarification on innoculation / incubation - Mushroom Cultivation

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Peace_Patrol
Rambling hippie

Registered: 04/22/02
Posts: 350
Loc: Electric Neverending
Last seen: 20 years, 5 months

Clarification on innoculation / incubation

#659306 - 06/02/02 03:40 PM (22 years, 7 months ago)

Hey everyone
I am just wanting some clarification on innoculation of the jars.
Ok, so at the moment, I have my sterilised jars. PF substrate till 1 inch from the top, then a layer of dry verm to the top. Three holes in each lid. Tinfoil covering lids still from sterilisation.
So, when I innoculate, I take off the tinfoil, but am I supposed to put it on again after injecting the spores? Or do I just leave it with them off?
Also, are the lids supposed to be screwed on all the way? (Tight)

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bassplayer74
old hand

Registered: 11/23/01
Posts: 906
Loc: Tennessee
Last seen: 14 years, 3 months

Re: Clarification on innoculation / incubation

[Re: Peace_Patrol]
#659313 - 06/02/02 03:45 PM (22 years, 7 months ago)

In reply to:

but am I supposed to put it on again after injecting the spores?

I'ts best but you probably wont need it since you put a whole inch of dry verm. on top. I think 1/4-1/2 inch is plenty.

In reply to:

Three holes in each lid.

Theres supposed to be 4 holes in each lid.

In reply to:

are the lids supposed to be screwed on all the way? (Tight)

Yes

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If you will it, it is no dream.

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powderfinger
member

Registered: 06/02/02
Posts: 112
Last seen: 21 years, 4 months

Re: Clarification on innoculation / incubation

[Re: Peace_Patrol]
#659320 - 06/02/02 03:52 PM (22 years, 7 months ago)

I have never had many problems with the pf jars becoming contaminated. For fast colonization, pull the foil off, inocculate, and leave the foil off for gass exchange. Use filter disks for superfast colonization if you can get'em. The only chances for contams are if you don't sterilze thourghly or if you get your syringe dirty.

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Shelter me from the powder and the finger,
cover me with the thought that pulled the trigger.

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Peace_Patrol
Rambling hippie

Registered: 04/22/02
Posts: 350
Loc: Electric Neverending
Last seen: 20 years, 5 months

Re: Clarification on innoculation / incubation

[Re: powderfinger]
#659730 - 06/02/02 11:13 PM (22 years, 7 months ago)

Thanks guys
By the way, it's not 'supposed' to be 4 holes per jar, you can do it with one, for reduced contamination chances, some do it with 2 but I chose 3 because then you still get the all-round spread but with 25% less interference with the jar's environment.

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Anno
Experimenter

Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 13 days, 3 hours

Re: Clarification on innoculation / incubation

[Re: Peace_Patrol]
#659801 - 06/03/02 12:13 AM (22 years, 7 months ago)

>you can do it with one, for reduced contamination chances

I don?t know where you snapped this.
It?s bullshit btw.

--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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