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Offlinebbtr
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Registered: 01/31/07
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Last seen: 1 year, 8 months
Liquid Culture without Agar - your input, please!
    #6518622 - 02/01/07 01:51 AM (14 years, 3 months ago)

Hi there!
I want to start a liquid culture but it seems I can't find agar where I live.
Also, I want to keep it for a longer time afterwards, that's why I suppose using honey wouldn't be preferable.

After searching FastFred's Media Cookbook (v0.97) Now 60 Recipes, Indexed!, I found Ragadinks Liquid Medium recipe. But it says very little:
" 16.5 g dextrose
1.5 g yeast
500 ml tap water [16] "

So, if someone has experience, please add to this! I'd like to use dextrose, avoid any hard-to-find stuff and keep it simple.

Thanks!


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Offlinethenewguy05
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Re: Liquid Culture without Agar - your input, please! [Re: bbtr]
    #6518628 - 02/01/07 01:54 AM (14 years, 3 months ago)

you don't need agar to start a LC. the honeywater tek stores just fine... i have cultures that are almost 3 years old and are as viable as they were the day i made them.

Mix 96ML or grams of warm water to 4ML or grams of honey and pressure cook like a pf jar for 15-20 minutes. as usual... longer is better but more than 20 minutes will carmelize the honey. when i say like a pf jar i mean cover the top with foil as to not throw off the moisture.


Edited by thenewguy05 (02/01/07 02:02 AM)


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InvisibleBlutjager
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Registered: 06/12/06
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Re: Liquid Culture without Agar - your input, please! [Re: thenewguy05]
    #6518655 - 02/01/07 02:09 AM (14 years, 3 months ago)

Yes,I have to agree,LC lasts forever,and no you don't need agar to make an LC.I cant even tell you how many great lc's I have made using nothing but water,honey and either a spore print or a spore syringe




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Offlinebbtr
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Registered: 01/31/07
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Re: Liquid Culture without Agar - your input, please! [Re: Blutjager]
    #6518701 - 02/01/07 02:37 AM (14 years, 3 months ago)

Thanks, guys!
I read some posts where people said that honey LCs can last for several weeks only, that's why I started searching for other options.
Guess I'll try honey too.

But in case anyone can contribute to Ragadinks Liquid Medium recipe, please do! It's always nice to have an alternative.


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Offlinethenewguy05
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Re: Liquid Culture without Agar - your input, please! [Re: bbtr]
    #6518707 - 02/01/07 02:43 AM (14 years, 3 months ago)

vacutainers are a nice long-term storage container.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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