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HUBSonDUBS


Registered: 09/01/05
Posts: 890
Last seen: 19 days, 9 hours
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Is this healthy growth on my agar?
#6489129 - 01/23/07 11:00 AM (17 years, 27 days ago) |
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This is about 4 or 5 days old it is PE LQ that I put onto some agar this is my first attempt but for some reason I dont think it went well someone reassure me.
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boogertool
Stranger
Registered: 08/26/06
Posts: 532
Loc: are you eyeballing me?
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Re: Is this healthy growth on my agar? [Re: HUBSonDUBS]
#6489133 - 01/23/07 11:02 AM (17 years, 27 days ago) |
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is that black mold in the top left? .... how come you didnt use the lc?
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RoachMan
Old Man



Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: Is this healthy growth on my agar? [Re: HUBSonDUBS]
#6489136 - 01/23/07 11:03 AM (17 years, 27 days ago) |
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Kind of hard to tell by the quality of the picture, however, it looks like there is some decent rhyzomorphic growth there.
If boogertool is right about the black mold...wrap it with a series of layers of duct tape before you go toss it in some misc. dumpster. You could try isolating the healthy growth onto a new dish, but I wouldn't open any contamination that looks black (or any for that matter).
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HUBSonDUBS


Registered: 09/01/05
Posts: 890
Last seen: 19 days, 9 hours
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Re: Is this healthy growth on my agar? [Re: boogertool]
#6489139 - 01/23/07 11:04 AM (17 years, 27 days ago) |
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Yea you are right I forgot to mention that I dont really mind that I figure its my first attempt but is that mycelium of cobweb. I plan on cloning I just didnt have any good fruits at the time so I put some LC in it.
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RoachMan
Old Man



Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: Is this healthy growth on my agar? [Re: HUBSonDUBS]
#6489157 - 01/23/07 11:13 AM (17 years, 27 days ago) |
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Oh, and when inoculating make sure you do it from the side of the dish without ever taking the cover off. Like this:

(if you are not already)
Oh, and cobweb mold doesn't form thick rhyzos like that. (Someone correct me if I'm wrong.)
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Is this healthy growth on my agar? [Re: RoachMan]
#6489200 - 01/23/07 11:30 AM (17 years, 27 days ago) |
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it looks like mycelium to me, though the picture is fuzzy
the black definitely appears to be a contaminate, notice the line of inhibition (the border where nothing wants to go) between the black and white.
i can assure you its definitely NOT cobweb... cobweb would be a pretty obvious grey and not look anything like that
LC is a pretty poor choice when it comes to inoculating agar, that extra moisture is not a desirable thing to have. normally you could get a clean sample from say the bottom right area to transfer to another dish, but with that moisture running around in there its more likely to spread the nasty stuff, rather than it being restricted to just a small area in the dish.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
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Re: Is this healthy growth on my agar? [Re: creamcorn]
#6489487 - 01/23/07 01:33 PM (17 years, 27 days ago) |
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In a glovebox you can easily get a tiny piece of good mycelium from that dish and move to a fresh one. Get the mycelium from the opposite side of the dish to the contaminant.
Once you have the mycelium on a new dish, about two to three days later, you'll see the mycelium crawling off the wedge to the new agar. At this point, transfer a tiny piece of the fresh growth to a new plate. If you make the second transfer before the mold germinates, you now have a clean culture. If not, continue doing transfers until you get ahead of the mold. It's easy to clean up cultures from contamination on agar.
You need to wrap your dishes to prevent contaminants getting under the lid. Remember, any time the air temperature in your room changes, the agar expands and contracts, forcing air in and out of the dish. This causes contamination if you don't have a gas permeable wrap around the edges such as parafilm. Some growers use cling wrap with success, but I stick with parafilm. At any rate, get the edges wrapped. Good luck. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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HUBSonDUBS


Registered: 09/01/05
Posts: 890
Last seen: 19 days, 9 hours
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Re: Is this healthy growth on my agar? [Re: RogerRabbit]
#6490020 - 01/23/07 04:48 PM (17 years, 27 days ago) |
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Yea thats what I figured im not too worried. Can I just put them into a clean glade Tupperware container instead of wrapping them?
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