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Offlineetam
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casing contam problems.
    #6470161 - 01/17/07 04:22 PM (17 years, 2 months ago)

Lately, everything I've cased has been getting trich. Just today I lost 4 casings. I'm using pasteurized MGMC as a casing material. Any idea what I'm doing wrong? Perhaps I didn't pasteurize it correctly. What is a good pasteurization method for casing layers? Any help is appreciated.

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Invisiblecreamcorn
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Re: casing contam problems. [Re: etam]
    #6470244 - 01/17/07 04:39 PM (17 years, 2 months ago)

are you getting it before fruiting, or after a flush or more?

does it seem like its on the top surface of the casing layer, or does it seem to run deeper like it might be possibly coming from underneath?

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InvisibleBlutjager
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Re: casing contam problems. [Re: creamcorn]
    #6470308 - 01/17/07 04:58 PM (17 years, 2 months ago)

I have always pasteurized my miracle grow the same way the pasteurization is done if you scroll down in this thread by Monster Mitch http://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=6194327&fpart=&PHPSESSID= it has always worked out good for me

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Offlineetam
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Re: casing contam problems. [Re: creamcorn]
    #6470311 - 01/17/07 04:59 PM (17 years, 2 months ago)

Quote:

creamcorn said:
are you getting it before fruiting, or after a flush or more?

does it seem like its on the top surface of the casing layer, or does it seem to run deeper like it might be possibly coming from underneath?




I'm getting it before knotting a few days after I put them in my greenhouse, which is humidified by a cool mist. It's on top of the casing layer, definitely not coming from underneath. Everything in the greenhouse that is uncased is doing fine. Flourishing even.

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Invisiblecreamcorn
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Re: casing contam problems. [Re: etam]
    #6470342 - 01/17/07 05:11 PM (17 years, 2 months ago)

odd. reasons i asked was because if you see mold BEFORE fruiting, it points to a less than healthy substrate... but if you say everything else is doing fine its probably ok to assume that isn't the case... if it were later flushes that would be a buffering thing

i don't know at any rate i've never had problems with mine early on like that except for times when the substrate was sketchy and probably doomed to fail

i both give mine a proper pasteurization (hydrated and placed in quart jars, treated to a hot water bath and watch for the temp to rise to 170, keep it stable there for an hour then cut the heat and leave it til morning - i do this in my PC because its also the biggest pot i have, but there's no pressure involved) and then i also more often microwave it - prep and hydrate, place in microwave safe container and cover, nuke 5 minutes, wait 15, nuke 3 more, wait 15 more, nuke 3 more finally and leave it til cool. both ways turn out fine for me and work about equally as well from what i can tell - the microwave one requiring less attention and effort IMO...

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Offlineetam
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Re: casing contam problems. [Re: creamcorn]
    #6470361 - 01/17/07 05:22 PM (17 years, 2 months ago)

I suppose I'll try the microwave thing and give it another go. I have some PF cakes available to crumble so I suppose I'll just try it on them. Thanks for the help cc.

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InvisibleMyPhilosophy
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Re: casing contam problems. [Re: creamcorn]
    #6470367 - 01/17/07 05:23 PM (17 years, 2 months ago)

Damn etam I'm sorry to hear about this bro. Hopefully it wasn't your PR strain, I knew you were pretty excited about that. Hopefully everything goes well and you figure your problem out. Best of luck man. :laugh:

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Offlineetam
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Re: casing contam problems. [Re: MyPhilosophy]
    #6470372 - 01/17/07 05:24 PM (17 years, 2 months ago)

Quote:

MyPhilosophy said:
Damn etam I'm sorry to hear about this bro. Hopefully it wasn't your PR strain, I knew you were pretty excited about that. Hopefully everything goes well and you figure your problem out. Best of luck man. :laugh:




It's not strain specific.  It's everything I'm working with.  I just threw out a Cambodian, B+, and Texas casing.  The PR's are still colonizing :wink:.  Along with my new OI's.

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InvisibleMyPhilosophy
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Re: casing contam problems. [Re: etam]
    #6470390 - 01/17/07 05:30 PM (17 years, 2 months ago)

Oh ok well I'm about to case also and hopefully my case doesn't catch the flu either. I will be using MGMC also.

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InvisibleBlutjager
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Re: casing contam problems. [Re: MyPhilosophy]
    #6470454 - 01/17/07 05:43 PM (17 years, 2 months ago)

I hate trich :crankey: I remember when all my jars used to get it,good luck Etam,I know how it feels

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Offlinetonyperez420
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Re: casing contam problems. [Re: Blutjager]
    #6470463 - 01/17/07 05:47 PM (17 years, 2 months ago)

ETAM

PC all your casings

I got tons of contams microwaving

GL

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Offlineetam
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Re: casing contam problems. [Re: tonyperez420]
    #6470494 - 01/17/07 05:57 PM (17 years, 2 months ago)

Quote:

tonyperez420 said:
ETAM

PC all your casings

I got tons of contams microwaving

GL




I've tried sterilizing my casing layer with the same results. My understanding is that you need the 'good bacteria' that is otherwise killed when you PC your casing layer to help ward off things like trich.

I know that many don't even pasteurize their casing layers with great results. Like RR, for example, in the thread discussing the advantages/disadvantages of casing and not casing.

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Offlineetam
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Re: casing contam problems. [Re: Blutjager]
    #6470723 - 01/17/07 06:43 PM (17 years, 2 months ago)

Quote:

Blutjager said:
I have always pasteurized my miracle grow the same way the pasteurization is done if you scroll down in this thread by Monster Mitch http://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=6194327&fpart=&PHPSESSID=  it has always worked out good for me




That is precisely how I've been pasteurizing mine... except I never hydrated the casing material beforehand.  I bet that's the problem.

:banghead:

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InvisibleBlutjager
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Re: casing contam problems. [Re: etam]
    #6470941 - 01/17/07 07:26 PM (17 years, 2 months ago)

Quote:

etam said:
Quote:

Blutjager said:
I have always pasteurized my miracle grow the same way the pasteurization is done if you scroll down in this thread by Monster Mitch http://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=6194327&fpart=&PHPSESSID=  it has always worked out good for me




That is precisely how I've been pasteurizing mine... except I never hydrated the casing material beforehand.  I bet that's the problem.

:banghead: 




Well what are you waiting for get out the old PC and hop to it  :hehehe:  :lol:

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Offlineetam
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Re: casing contam problems. [Re: Blutjager]
    #6471006 - 01/17/07 07:43 PM (17 years, 2 months ago)

Quote:

Blutjager said:
Quote:

etam said:
Quote:

Blutjager said:
I have always pasteurized my miracle grow the same way the pasteurization is done if you scroll down in this thread by Monster Mitch http://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=6194327&fpart=&PHPSESSID=  it has always worked out good for me




That is precisely how I've been pasteurizing mine... except I never hydrated the casing material beforehand.  I bet that's the problem.

:banghead: 




Well what are you waiting for get out the old PC and hop to it  :hehehe:  :lol:




*sigh*
Harvesting a couple uncased trays right now, then I'll get the PC going.  Gonna crumble up a few Cambodian and A Strain PF cakes I have laying around in the fridge and give it another go.

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InvisibleBlutjager
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Re: casing contam problems. [Re: etam]
    #6471250 - 01/17/07 08:46 PM (17 years, 2 months ago)

Quote:

etam said:
Quote:

Blutjager said:
Quote:

etam said:
Quote:

Blutjager said:
I have always pasteurized my miracle grow the same way the pasteurization is done if you scroll down in this thread by Monster Mitch http://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=6194327&fpart=&PHPSESSID=  it has always worked out good for me




That is precisely how I've been pasteurizing mine... except I never hydrated the casing material beforehand.  I bet that's the problem.

:banghead: 




Well what are you waiting for get out the old PC and hop to it  :hehehe:  :lol:




*sigh*
Harvesting a couple uncased trays right now, then I'll get the PC going.  Gonna crumble up a few Cambodian and A Strain PF cakes I have laying around in the fridge and give it another go.




Any advice for fruiting uncased,Hippie chick has got me thinking about trying it again but last time I could never get the damn thing to pin

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Offlinetonyperez420
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Re: casing contam problems. [Re: etam]
    #6471294 - 01/17/07 08:57 PM (17 years, 2 months ago)

Quote:

etam said:
Quote:

tonyperez420 said:
ETAM

PC all your casings

I got tons of contams microwaving

GL




My understanding is that you need the 'good bacteria' that is otherwise killed when you PC your casing layer to help ward off things like trich.






Dont be tricked into trying new ideas..
Casings are suppose to be of ZERO nutritive value
they are suppose to "case" the moisture so it is held in your substrate
there is no such a thing as "good bacteria" in casings
if you dont believe me check out TMC Stamets Pg 130

Try using more orthodox methods, especially procedures used by proven growers...

as far as casings go

when I microwaved for 10 mins=
50/50 contam

pasteurized= VERY high contam rate, waste of my time

PC at 15 psi anywhere from 30 - 90 mins
its been pretty perfect

I do have a flow hood... you may want to check the cleanliness of your FC setup while adding casing

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Offlinetonyperez420
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Re: casing contam problems. [Re: etam]
    #6471314 - 01/17/07 09:01 PM (17 years, 2 months ago)

Quote:

etam said:

I know that many don't even pasteurize their casing layers with great results.




again, there is only 1 outspoken member that speaks highly in this regard, RR

PC your casings

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Offlineetam
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Re: casing contam problems. [Re: tonyperez420]
    #6471367 - 01/17/07 09:12 PM (17 years, 2 months ago)

I will pasteurize my casings this time around. Thanks for the input.

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InvisibleMajorDick
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Re: casing contam problems. [Re: etam]
    #6471402 - 01/17/07 09:19 PM (17 years, 2 months ago)

Alas, there is no correct way to prepare a casing.
Every grow room/home is essentially different unless you have a 'clean room'. The mold/bac counts are never the same from place to place. The same goes for the casing material itself.

If pasteurizing it didn't work. Then, by all means, try sterilizing it next time or leaving it untreated.

If the infections still persist I would think it's time for some vigorous house cleaning.

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Offlineetam
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Re: casing contam problems. [Re: MajorDick]
    #6471442 - 01/17/07 09:28 PM (17 years, 2 months ago)

Quote:

MajorDick said:
Alas, there is no correct way to prepare a casing.
Every grow room/home is essentially different unless you have a 'clean room'. The mold/bac counts are never the same from place to place. The same goes for the casing material itself.

If pasteurizing it didn't work. Then, by all means, try sterilizing it next time or leaving it untreated.

If the infections still persist I would think it's time for some vigorous house cleaning.




I completely agree. The last time I pasteurized my casing material I failed to hydrate it before pasteurizing it. I'm pretty sure this is my problem, which is why I'm trying pasteurization again. I had a failed run with sterilizing the casing material before as well, but I also did not hydrate it prior to sterilizing it.

If I still can't get it to work, house cleaning is indeed in store. I'm also moving soon, so I'll be sure to make sure the new place is spotless and as sporeless as possible.

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Offlinetonyperez420
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Re: casing contam problems. [Re: etam]
    #6471473 - 01/17/07 09:37 PM (17 years, 2 months ago)

Quote:

etam said:
I will pasteurize my casings this time around. Thanks for the input.




DONT PASTEURIZE, PRESSURE COOK!

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Offlineatomic1
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Re: casing contam problems. [Re: etam]
    #6471488 - 01/17/07 09:41 PM (17 years, 2 months ago)

You said you were using a cool mist.....if it is a "wick" type humidifier then its possible that the wick is spreading contams since its paper like and stays wet. Someone else was talking about this the other day. I have a wick type myself but don't use it for growing.

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Offlineetam
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Re: casing contam problems. [Re: tonyperez420]
    #6471489 - 01/17/07 09:41 PM (17 years, 2 months ago)

Quote:

tonyperez420 said:
Quote:

etam said:
I will pasteurize my casings this time around. Thanks for the input.




DONT PASTEURIZE, PRESSURE COOK!




How about I do both? One jar each? :P

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InvisibleMajorDick
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Re: casing contam problems. [Re: etam]
    #6471509 - 01/17/07 09:48 PM (17 years, 2 months ago)

I kinda had a problem with pasteurizing.
I hydrated a mix inside a large ziplock bag, and guess what happened.
The fucking bag came open and saturated my mix.
Oh well.
I stab the bag a dozen or so times with my thermoprobe, and say "Fuck it! It'll just go quicker."!
Working with time constraints I get to squeeze a HOT ASS BAG down to filed capacity. Tossed that fucker in a trash bag, tied it up, and called it a day.
No contams.. And it was coir so I guess that says something. (nowhere near the cleanest place either)


HC says in a concurrent thread that she hydrates her casings with a spray bottle AFTER she applies them to the substrate.
I think I'm gonna try that eventually.

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