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OfflineHUBSonDUBS
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Let me get this right, questions with agar and cloning
    #6449862 - 01/11/07 07:56 PM (14 years, 3 months ago)

I have some agar dishes and I want to clone, so I pick a mushroom preferably one that is in a cluster and large and cut with a clean knife a small piece towards the bottom and place that in my agar dish. When the agar dish colonized I cut out another small piece that has more of a vein look to it then a puffy look and place that into a premaid LC. Anyone have any comments?


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Offlinedist0rt
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Re: Let me get this right, questions with agar and cloning [Re: HUBSonDUBS]
    #6449922 - 01/11/07 08:12 PM (14 years, 3 months ago)

Well.. sounds like you got the jist of it. Follow sterile technique.


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OfflineRogerRabbitM
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Re: Let me get this right, questions with agar and cloning [Re: dist0rt]
    #6450199 - 01/11/07 09:43 PM (14 years, 3 months ago)

Don't wait for the first dish to fully colonize. As soon as it begins to grow, transfer mycelium away from the point of cloning to new dishes. This way, you can isolate healthy mycelium away from contaminants. You won't be doing strain isolation on a clone, so don't bother looking for rhizomorphs. Just get healthy mycelium.
RR


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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