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Offlinesoulman1990
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spore germination
    #6427938 - 01/05/07 01:02 PM (17 years, 4 months ago)

I was wondering when mycelium is growing on grain from a multi spore inoculation is all the mycelium genetically the same or do different spores carry different traits of the parent. Does stamets book deeply cover the biology of mycelium reproduction.

Thanks much, mike

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Invisiblefastfred
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Re: spore germination [Re: soulman1990]
    #6427982 - 01/05/07 01:15 PM (17 years, 4 months ago)

> is all the mycelium genetically the same

No. It will all have the same parents, but you can expect the same variation that you would see between siblings. That is to say that all the substrains will share 50% of their genes.

There can be quite a bit of variation between brothers/sisters.


-FF

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Offlinesoulman1990
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Re: spore germination [Re: soulman1990]
    #6428241 - 01/05/07 02:27 PM (17 years, 4 months ago)

well, the real question is what determines the mycelias genetics from the multispore solution.

Edited by soulman1990 (01/05/07 02:28 PM)

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Invisiblefastfred
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Re: spore germination [Re: soulman1990]
    #6428248 - 01/05/07 02:29 PM (17 years, 4 months ago)

50% from each parent.

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Offlinesoulman1990
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Re: spore germination [Re: fastfred]
    #6428398 - 01/05/07 03:08 PM (17 years, 4 months ago)

when you say parent do you mean spore. if so are there several mycelia families in my jars

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OfflineRogerRabbitM
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Re: spore germination [Re: fastfred]
    #6428408 - 01/05/07 03:10 PM (17 years, 4 months ago)

Of course there's thousands of parents if thousands of spores germinate from multispore inoculation. Eventually, most of the substrains combine into a single organism through the process of anastomosis.

If one wishes to separate out the individual strains, or substrains as they're sometimes called in order to preserve the diversity, you want to place the multispore culture on agar where you can observe the growth in the flat, two dimensional plane of the Petri dish. This process needs to begin within a day or two of germination so you can catch the individual strains before they combine with other dikaryons via anastomosis.

Some strains are not compatible, so this is the reason on many substrates that were inoculated by multispore you'll see drastically different looking(and performing) mushrooms on the same flush. It's because those that are not compatible usually lay claim to their niche of real estate and fruit from there.
RR


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Re: spore germination [Re: RogerRabbit]
    #6429953 - 01/06/07 02:12 AM (17 years, 4 months ago)

I wonder how common anastomosis is. I can't imagine that substrains of different mating types would form successful junctions.

Once anastomosis has occurred they must simply share the mycelial network. Both individuals would still be present in the media.

I would be interested to see how clamp connections form near the joint. Do you have any observations on this RR?


-FF

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OfflineRogerRabbitM
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Re: spore germination [Re: fastfred]
    #6433246 - 01/07/07 12:25 AM (17 years, 4 months ago)

No. By the time it gets to that point, my liquid slides are so thick with growth, the microscope light can't shine through them. According to stamets, and it seems to match my observation, most of the time all the substrains combine into a coherent whole. In other words, if you took a sample of mycelium from each corner of a fruiting tray and placed them on the same petri dish, they'd grow together seamlessly without forming a zone of inhibition.

Still, there are times when it's fairly obvious by observation that more than one substrain is at work in the same fruiting tray.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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