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OfflineBlueJay
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Re: HUGE mexicana sclerotia :) [Re: Una]
    #639113 - 05/21/02 10:35 AM (20 years, 10 months ago)

Ok so if someone wants to continue those genetics would you cut a piece from the best sclerotia, then get it back to veg state then fruit and collect spores? How would one go about getting that piece of sclerotia to go back into growth and possible fruit as is????? Ordered strain A yesterday....... Gonna work on this....Whats the standard for getting the Mexicans to fruit...Lent out the TMC for a few weeks so I cant refer back...Being the good guy I am...J


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Respect all, Fear None!
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Cacti are my new life.....


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OfflineAnnoA
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Re: HUGE mexicana sclerotia :) [Re: BlueJay]
    #639252 - 05/21/02 12:12 PM (20 years, 10 months ago)

>How would one go about getting that piece of sclerotia to go
>back into growth and possible fruit as is?????

Cut a piece of the sclerotia inner tissue and place on agar. It will revert to vegetative growth again, then you can use this mycelium as your stock culture.


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OfflineBlueJay
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Re: HUGE mexicana sclerotia :) [Re: Anno]
    #639262 - 05/21/02 12:24 PM (20 years, 10 months ago)

That was the exact procedure that I was thinking of SIR ANNO....just to lazy to type all that...But wanted to know if I was on track in my thinking....Do you have much experience with P. Mexican,,,Anno?


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Respect all, Fear None!
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Cacti are my new life.....


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OfflineAnnoA
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Re: HUGE mexicana sclerotia :) [Re: BlueJay]
    #639305 - 05/21/02 01:08 PM (20 years, 10 months ago)

>Do you have much experience with P. Mexican,,,Anno?

Much? No, not much. I fruited them once and I have some jars with sclerotia going.
But I tried the aforementioned procedure with tampanesis scerotia and it turned out ok.


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Offlinedumlovesyou
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Re: HUGE mexicana sclerotia :) [Re: Anno]
    #640293 - 05/22/02 09:07 AM (20 years, 9 months ago)

Can you do this with spores?? I don't master the agar tehnique yet. For example, inoculate spores instead of mycelium?


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OfflineAnnoA
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Re: HUGE mexicana sclerotia :) [Re: dumlovesyou]
    #641037 - 05/22/02 09:12 PM (20 years, 9 months ago)

Yes, you can.


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OfflineBlueJay
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Re: HUGE mexicana sclerotia :) [Re: Anno]
    #641521 - 05/23/02 08:33 AM (20 years, 9 months ago)

{Cut a piece of the sclerotia inner tissue and place on agar. It will revert to vegetative growth again, then you can use this mycelium as your stock culture.}

I was really trying to see if anyone had a tech to fruit the sclertia directly...In nature these are produced to withstand unideal conditions then when its optimal growth continues. Now would it start back in veg or would it fruit immediately to reproduce???Any ideas???whats the experience on these like ???more energetic or drunk wobbley>Closer to a pan buzz?????Thanks.




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Respect all, Fear None!
The Peacemaker

Cacti are my new life.....


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