|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
psily_sarah
Stranger
Registered: 12/28/06
Posts: 3
Last seen: 15 years, 4 months
|
sclerotia question - please help!!
#6405921 - 12/28/06 05:52 PM (17 years, 1 month ago) |
|
|
Hello all! I have been viewing these forums for some time but have only just registered.
I have a question concerning sclerotia forming species (psilocybe atlantis, in particular): Is it possible to use the sclerotia to colonize new substrates? I shall be growing the sclerotia on a brown rice substrate from a (purchased) spore syringe, but am unsure about carrying-on the growing process once the sclerotia have formed.
My thoughts were that i could select the largest sclerotia, remove a segment from its center, under sterile conditions, and use this to colonize new substrate jars. Is this possible? I assume grain-to-grain transfer can be done, but is there a way to select only the best-growing "strain"? (if that's the right word).
I suppose i could add a casing layer to grow the mushrooms and proceed as normal, but i was wondering whether this was necessary. Apologies if this has been covered elsewhere, i have been unable to find such information. Any help greatly appreciated!
Sarah xx
|
Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
Last seen: 10 years, 8 months
|
Re: sclerotia question - please help!! [Re: psily_sarah]
#6405959 - 12/28/06 06:07 PM (17 years, 1 month ago) |
|
|
Hem.... I do believed I answered this exact same question at mycotopia....
You want me to say it again?
--------------------
Ronhero on the Growery is me
|
Helltick
Player


Registered: 10/30/05
Posts: 684
Loc: Deep...
|
Re: sclerotia question - please help!! [Re: psily_sarah]
#6405963 - 12/28/06 06:08 PM (17 years, 1 month ago) |
|
|
I've grown p.mexicana-A in 1 quart rye jars...dont know about using the actual sclerotia to knock up more jars...I just wait til jar is fully colonized (prior to any sclerotia forming) and knock up more jars via G2G.
-------------------- My Agar Tek. My Greenhouse Tek. "It's not good when it's done, it's done when it's good"
|
Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
Last seen: 10 years, 8 months
|
Re: sclerotia question - please help!! [Re: Helltick]
#6405974 - 12/28/06 06:11 PM (17 years, 1 month ago) |
|
|
Question from mycotopia:
Quote: Originally Posted by phungus View Post Is it possible to use the sclerotia to colonize new substrates?
Yes, but it is not exacxtly that simple
Quote: Originally Posted by phungus View Post My thoughts were that i could select the largest sclerotia, remove a segment from its center, under sterile conditions, and use this to colonize new substrate jars. Is this possible?
Yea but I would suggest that you first clone it on agar
Quote: Originally Posted by phungus View Post I suppose i could add a casing to grow the mushrooms and proceed as normal, but i was wondering whether this was necessary.
For atlantis to fruit you need a casing layer. 4:1 peat / calcium carbonate for your casing . Note: You want real calcium carbonate not crushed oyster shell for the simple fact that crushed oyster takes too long to break down and is used as a long term buffer not short.
--------------------
Ronhero on the Growery is me
|
psily_sarah
Stranger
Registered: 12/28/06
Posts: 3
Last seen: 15 years, 4 months
|
Re: sclerotia question - please help!! [Re: Babo911]
#6406022 - 12/28/06 06:28 PM (17 years, 1 month ago) |
|
|
haha, you're right babo911! I just wondered what people here had to say on the matter. Nothing personal!! Just trying to get as many opinions as possible. Thanks for your response(s!!) by the way =) I should have guessed that a lot of the same people are at both sites =P Ah well!
Helltick, do you notice a loss of potency over time with this method? (i think that i read elsewhere that psilocybin content decreases the more one does grain-to-grain transfers).
Also i wonder whether either of you have experience with growing this strain (or other sclerotia-forming stains)on brown rice? I know that rye grass seed is supposedly the best for sclerotia but i can't seem to find any locally... brown rice will have to do!! Interested to hear your thoughts on this..
Thanks for the quick response =)
|
Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
Last seen: 10 years, 8 months
|
Re: sclerotia question - please help!! [Re: psily_sarah]
#6406072 - 12/28/06 06:48 PM (17 years, 1 month ago) |
|
|
Rye grass seed is seccond to rye berries aka winter rye.
I buy mine 50 lbs for $6 plus tax


They are from a few weeks ago but that is my sclerotia as of now (atlantis #7)
You get no loss in anything grain to grain. And youre better off with white rice then brown from what I have read.... Don't know why
--------------------
Ronhero on the Growery is me
|
psily_sarah
Stranger
Registered: 12/28/06
Posts: 3
Last seen: 15 years, 4 months
|
Re: sclerotia question - please help!! [Re: Babo911]
#6406184 - 12/28/06 07:21 PM (17 years, 1 month ago) |
|
|
Babo911, i have a few questions if you wouldn't mind helping me out: 1) Do you use organic rye, or is normal rye ok? 2) I also wondered how you prepare the rye. Is it the same as for cubensis (with 250ml rye and 180ml water for a quart (1000ml) jar? - pressure cooked at 15psi for an hour?) 3) Is 25 C the best temperature for growth (as i have read) or have you found it to be better at a higher temperature? 4) what kind of yield would one get from a quart jar? 5) I'm also interested in what the trip is like... is it similar to mexicana sclerotia or any other mushroom trip that you could compare it to?
sorry for all the questions, this is my first sclerotia grow and i just want to make sure all goes well.
|
Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
|
Re: sclerotia question - please help!! [Re: psily_sarah]
#6406217 - 12/28/06 07:30 PM (17 years, 1 month ago) |
|
|
I have always been fascinated by sclerotia,I mean whats not to like,jars sitting in your closet for endless months on end and next yr when they are ready they taste all good and crunchy.....IL take my cubes any day,when I'm moving to different species I'm going to pans or even edibles,sclerotia seems wasteful to me,not material wasteful,just allot of time
|
Brainiac
Rogue Scientist


Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋
|
Re: sclerotia question - please help!! [Re: Blutjager]
#6406416 - 12/28/06 09:05 PM (17 years, 1 month ago) |
|
|
Quote:
Blutjager said: I have always been fascinated by sclerotia,I mean whats not to like,jars sitting in your closet for(about 3mouths) endless months on end and next yr when they are ready they taste all good and crunchy.....IL take my cubes any day,when I'm moving to different species I'm going to pans or even edibles,sclerotia seems wasteful to me,not material wasteful,just allot of time
Have you tryed doing a search, on this web page .There a lot of info on it. If I where doing I would make four jars every mouth,so you have a new jars ready for you. 1) organic rye,your eating some thats grow on it. 2) I also wondered how you prepare the rye. Is it the same as for cubensis (with 250ml rye and 180ml water for a quart (1000ml) jar? - pressure cooked at 15psi for an hour?)yes 3) Is 25 C the best temperature for growth (as i have read) or have you found it to be better at a higher temperature?Room temp 70-75f 4) what kind of yield would one get from a quart jar?120g wet at 30% around 40 dry 5) I'm also interested in what the trip is like... is it similar to mexicana sclerotia or any other mushroom trip that you could compare it to?Unknown 
Edited by Brainiac (12/28/06 09:13 PM)
|
Dem_Bones
Strangler



Registered: 02/12/05
Posts: 307
Loc: Oklahoma
Last seen: 1 year, 10 months
|
Re: sclerotia question - please help!! [Re: Brainiac]
#6406558 - 12/28/06 09:55 PM (17 years, 1 month ago) |
|
|
Quote:
Brainiac said: 1) organic rye,your eating some thats grow on it.
Oh thats just kinda silly what is the difference between rye and organic rye ? about 50 bucks what the hell is the point you can get a 50 lbs bag of clean rye grain from a CO-OP or feed store for 5 to 10 Bucks why pay any more its sold as feed.
and further more organic food waste land, it take almost twice as much land to grow the same about of crops with out fertilizers than it does with. If all are food was grown that way there would be no room left for forest and wildlife because it would all be wasted on farm land, and you would use alot more fuel driving farm equipment all over the need land, eat organics food if you are scared there could be pesticides on it or what ever, but don't to if you think it help the environment because it does not, all it does is use more land and make food more expensive. see that pic down there if it were not for man made chemical fertilizer from natural minerals in order to make that much food the amount of farm land would have to increase just as much. More farm land = less natural habitat bottom line. chemical fertilizer saves billions of acres of wild natural habitat from becoming farm land. the world has 6.5 billion hungry people to feed.
-------------------- At the bottom of this page is a search bar to search all the post and threads on the forum so you can see all the other 500 people who asked the same thing.use the top search too it has shroomery approved FAQs USE IT YOU CAN DO IT !
Edited by Dem_Bones (12/28/06 09:57 PM)
|
Helltick
Player


Registered: 10/30/05
Posts: 684
Loc: Deep...
|
Re: sclerotia question - please help!! [Re: Dem_Bones]
#6406639 - 12/28/06 10:33 PM (17 years, 1 month ago) |
|
|
The problem with sclerotia is not only the time it takes (months). ...Rather, harvesting this shit is just a bitch...not just like picking a tray.
Been there done that...and IMHO its a waste of time,unless your hardcore and really want some stones.
Good luck tho!
-------------------- My Agar Tek. My Greenhouse Tek. "It's not good when it's done, it's done when it's good"
|
Rahz
Alive Again


Registered: 11/10/05
Posts: 9,252
|
Re: sclerotia question - please help!! [Re: psily_sarah]
#6406656 - 12/28/06 10:38 PM (17 years, 1 month ago) |
|
|
If I wanted to clone sclerotia, I would put the jar in a glove box along with a liquid culture medium, and transfer a nugget over.
Sclerotia aren't a bad option in some situations. It's the ultimate stealth grow. Yield per jar isn't all that bad either. They make good tea, the same as fruits do.
Tip I found, when you remove your jars from the PC, give them a good shake. This will cause some of the material to stick to the glass above the substrate level, which causes the mycelium to climb the glass, which causes a large portion of the sclerotia to form above the substrate... which means you can just open the jar and pick them out. Would be ideal for a jar to LC transfer.
-------------------- rahz comfort pleasure power love truth awareness peace "You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi
|
RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
|
Re: sclerotia question - please help!! [Re: Dem_Bones]
#6406660 - 12/28/06 10:39 PM (17 years, 1 month ago) |
|
|
Let's can the off topic preaching please. The chemicals and salts in man made fertilizers trash the land and pollute the rivers, killing fish and rendering previously good soil barren. There's two sides to every story, and I'm sure a forum for them too, but this isn't it.
Sclerotia is not a waste of time at all. I can grow half a quart of sclerotia from one quart jar of rye berries in 8 weeks. The key is to hydrate the berries in weak coffee, and remember to add a pinch of gypsum to each jar, or use it in the soak water for the grains.
It's easy to isolate for a good sclerotia producer on agar, and use that strain to inoculate jars for producing stones. You can also isolate for fruiting qualities and use that strain for generating prints/fruits. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 1 month, 19 days
|
Re: sclerotia question - please help!! [Re: RogerRabbit]
#6406726 - 12/28/06 10:56 PM (17 years, 1 month ago) |
|
|
I've heard Paul mention harvesting stones and simply burying them in potting soil for fruiting. Although it's a time consuming process, the technique looks very sound. It may worth looking into. Or you can use rye grass seed and gypsumed water at a ratio of 2:1 , mixed well in the jars and then pc'd for an hour. Then inoculate the jars and await colonization. Make sure the colonization is complete. By that I mean; there's an initial colonization of the grain that's very fast, yet isn't thick as it should be. Just wait another 10 days or so for the initial colony to thicken up with pure, white mushroom mycelium. Then you can transfer that grain into a container for fruiting. Allow the grain a week or so it can heal up from the transfering process. Then case with peat based potting soil or peat alone and allow that to colonize. Once colonized introduce into fruiting conditions that consist of 95% RH and a temperature of 77-80 degrees.
Edited by Hotnuts (12/28/06 11:02 PM)
|
Helltick
Player


Registered: 10/30/05
Posts: 684
Loc: Deep...
|
Re: sclerotia question - please help!! [Re: RogerRabbit]
#6406811 - 12/28/06 11:31 PM (17 years, 1 month ago) |
|
|
Dunno Man...But harvesting stones from rye jars, kinda reminds me of sifting through a kitty litter box searching for shit.
Grow some real fruits and pick the trays...much better yeilds for your hard earned time.
GL.
-------------------- My Agar Tek. My Greenhouse Tek. "It's not good when it's done, it's done when it's good"
|
Brainiac
Rogue Scientist


Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋
|
Re: sclerotia question - please help!! [Re: Helltick]
#6406821 - 12/28/06 11:37 PM (17 years, 1 month ago) |
|
|
you could do both fruit it and have some stones
--------------------
Fair is Fair
|
|