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Offlinerokin
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Registered: 12/24/06
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1st timer Australian
    #6402082 - 12/24/06 04:04 PM (14 years, 4 months ago)

Hello everyone,
This is my first time cultivating mushrooms. And im not even so sure about the strain im using; Anyone with information regarding Australian? Is it Cubensis?
Anyways I used 8 sterilizied (pressure cooker) glasses with vermiculite, rice flour, and distilled water. then used tin foil as lids. Made 2 inoculation holes and about 1-1.5 cc per Glass.
I also used distilled water making the syringe.
The glasses are incubating 3 days now at 27-28c degrees and i think i already see some fuzzy mycelium that im not so sure about.A picture wont be comprehendable enough. For now, no contams are visible.
Wish me luck guys.. ill be back with pics as soon as possible i hope
thanks


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Offlinegoodolgoogy
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Re: 1st timer Australian [Re: rokin]
    #6402089 - 12/24/06 04:07 PM (14 years, 4 months ago)

You aren't sure if it's Cubensis? Didn't it say on the place you bought it from?


--------------------
RogerRabbit said:
Don't assault your mycelium with peroxide.  That's like throwing acid in your girlfriends face just to see if it sizzles.


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Offlinerokin
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Re: 1st timer Australian [Re: goodolgoogy]
    #6402137 - 12/24/06 04:25 PM (14 years, 4 months ago)

I got then from the FSRE. It only had AUSTRALIAN written on it.
I think it is cubensis though...


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Offlineetam
it's a hobby...
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Re: 1st timer Australian [Re: rokin]
    #6402238 - 12/24/06 05:16 PM (14 years, 4 months ago)

It's probably cubensis. Did you you sterilize the distilled water before you made the syringe?

Good luck!


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Offlinerokin
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Re: 1st timer Australian [Re: etam]
    #6402845 - 12/27/06 07:12 PM (14 years, 4 months ago)

sure
i was as sterile as i could
here are pictures from the fifth day
http://img218.imageshack.us/img218/5057/dsc00987he4.jpg
http://img220.imageshack.us/img220/8505/dsc00985ay8.jpg
http://img220.imageshack.us/img220/8086/dsc00990rr9.jpg
http://img336.imageshack.us/img336/4963/dsc00989mp3.jpg

today is the 6th day and out of 8 glasses i inoculated 5 have mycelium on them, its kinda fuzzy mycelium as you can see but im happy cuz this is my first time.
i dont know what to do with the 3 other glasses... should i wait to see if mycelium shows up on them or pass?
i also inoculated yesterday two jars of rye seed and one PF cake with Thai spores. The spores were weird, they werent really visible like the Australian. I hope i did it right.
Well, tell me what you think people, i cant wait till i see the first fruit :laugh: haaaa


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Invisibletakk
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Re: 1st timer Australian [Re: rokin]
    #6403073 - 12/27/06 08:50 PM (14 years, 4 months ago)

there's aussie psilocybe cubensis, psilocybe subcubensis (known locally as "gold caps" and/or "gold tops"), psilocybe subaeruginosa, and copelandia cyanescens (the latter is known locally as "blue meanies")

anyways, good luck with the grow dude :thumbup:
pity FSRE doesn't shed much light on their website either:

http://www.fsre.nl/index.php?option=com_content&task=view&id=50&Itemid=57

You should def. follow these babies through to fruiting and give them a nice big picture! :grin:


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Offlinegoodolgoogy
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Re: 1st timer Australian [Re: takk]
    #6403083 - 12/27/06 08:54 PM (14 years, 4 months ago)

So far so good. Keep em incubated and keep an eye out for any contamination.


--------------------
RogerRabbit said:
Don't assault your mycelium with peroxide.  That's like throwing acid in your girlfriends face just to see if it sizzles.


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InvisibleBlutjager
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Re: 1st timer Australian [Re: goodolgoogy]
    #6403256 - 12/27/06 10:06 PM (14 years, 4 months ago)

I bet its a cube strain


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Offlinerokin
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Re: 1st timer Australian [Re: Blutjager]
    #6403963 - 12/28/06 05:58 AM (14 years, 4 months ago)

ummm
well thanks for all the feedback
but i still have a few questions.
Now i know almost for sure after 7 days that 5 jars are with mycelium and 3 others arent.
Im almost sure mycelia wont develeop in these 3 jars anymore cuz its been a week y'know..... should i discard them?
Oh and another thing... Im using the fish tank heater method ti incubate, i set the heat for 30 degrees cuz i think that if i put it on 27-28 its actually lower than that.
some new pics...
http://img208.imageshack.us/img208/4985/dsc01011xr0.jpg - first jar
http://img207.imageshack.us/img207/8708/dsc01013ne2.jpg - second jar
http://img214.imageshack.us/img214/7130/dsc01016di2.jpg - third jar
http://img413.imageshack.us/img413/8495/dsc01018kf8.jpg - fourth jar
the fifth doesnt have quite visible enough mycelium so i didnt take pictures
No visible contams on any of the five jars as you can see.
The newley inoculated Rye jars with Thai spores are at 2nd day incubating and are still showing no mycelium.
Check it out, thanks


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Invisibletakk
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Re: 1st timer Australian [Re: rokin]
    #6403997 - 12/28/06 07:19 AM (14 years, 4 months ago)

whoa buddy, one week is one week - it might take that whole week just for two spores from your syringe/print to find eachother and make sweet holy matrimony all over your uncle's rye grain/substrate a la miscelania  :humpme:

in any case, just keep the 3 you want to throw away for another week! In the mean time, why not knock up some honey lc to pass the time? :hook:


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Offlinerokin
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Re: 1st timer Australian [Re: takk]
    #6410182 - 12/30/06 12:03 PM (14 years, 4 months ago)

sup
I recently noticed strange bluish-greenish-geryish discolorations on some of my jars. Im not sure if these are contaminations. It also looks to me maybe theyre too wet or humid.
Here is a pictue of one of the jars.
http://img413.imageshack.us/img413/5959/dsc01043tv4.jpg
what do you think?
I'll upload some new pictures soon. I also started another culture of Thai where mycelium occured pretty fast.


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InvisibleBlutjager
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Re: 1st timer Australian [Re: rokin]
    #6411785 - 12/30/06 11:35 PM (14 years, 4 months ago)

The color on mt monitor is a bit off but from what I can see I recommend you toss them,sorry man but that doesn't look too healthy to me


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Offlinerokin
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Re: 1st timer Australian [Re: Blutjager]
    #6412624 - 12/31/06 05:01 AM (14 years, 4 months ago)

yeah i dont know i think ill keep em a few more days just to see but ill seperate them from the healthy jars.
The mycelium in this jar is really weird instead of being fuzzy or strainy rhizomorphic it looks like patches of mycelium. YUCK
:P


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Offlinerokin
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Re: 1st timer Australian [Re: rokin]
    #6412793 - 12/31/06 07:44 AM (14 years, 4 months ago)

Anyways. For better news, i have new pics of all of my 10 jars.

JAR 1 - this jar has been incubating for more than a week but still mycelium visible. No contams visible
http://img69.imageshack.us/img69/883/jar1cn7.jpg
http://img69.imageshack.us/img69/5382/jar12qh0.jpg
http://img69.imageshack.us/img69/8039/jar13bb1.jpg

JAR 2 - Check this jar.does it looks contaminated ?
http://img69.imageshack.us/img69/9331/jar2wy1.jpg
http://img372.imageshack.us/img372/1286/jar21bx4.jpg
http://img482.imageshack.us/img482/3061/jar22oi2.jpg
http://img490.imageshack.us/img490/2208/jar23tb7.jpg

JAR 3 - Another jar thats showing neither mycelium nor contams.
http://img482.imageshack.us/img482/1958/jar3zq3.jpg
http://img221.imageshack.us/img221/7567/jar31qn3.jpg
http://img461.imageshack.us/img461/650/jar32tm9.jpg

JAR 4 - nice strainy mycelium starting to form in top of Jar. No contams visible.
http://img461.imageshack.us/img461/2157/jar4fy2.jpg
http://img482.imageshack.us/img482/4976/jar41ic6.jpg
http://img461.imageshack.us/img461/7945/jar42jz0.jpg

JAR 5 - Another suspicious jar with mycelium and chance for
contaminations.
http://img482.imageshack.us/img482/2295/jar5da1.jpg
http://img461.imageshack.us/img461/6508/jar51ya6.jpg

JAR 6 - This jar has allot of mycelium already. No contams visible. Looking good all in all.
http://img222.imageshack.us/img222/4946/jar6kk3.jpg
http://img222.imageshack.us/img222/9035/jar61lw6.jpg

JAR 7 - Another good looking jar with mycelium and no visible contams
http://img214.imageshack.us/img214/6320/jar7ur9.jpg
http://img222.imageshack.us/img222/159/jar71ll7.jpg
http://img222.imageshack.us/img222/6697/jar72na4.jpg

JAR 8 - Suspicious Jar. Is this mycelium? maybe it is but it looks a little bit nasty.
http://img222.imageshack.us/img222/700/jar8jj2.jpg
http://img482.imageshack.us/img482/9689/jar81de4.jpg

JAR 9 - This one is a different culture, this is a Thai cake. Incubated for about 5 days its looking pretty good and colonizing pretty fast. Here also no contams are visible.
http://img482.imageshack.us/img482/1701/thaifront2ry5.jpg
http://img222.imageshack.us/img222/5485/thaibackvh3.jpg

RYE JAR - This jar with rye is also Thai and also been incubating for 5 days. this pic is from yesterday. Today it looks even better.
http://img222.imageshack.us/img222/5135/rye1me0.jpg
http://img222.imageshack.us/img222/7449/rye2jw0.jpg
http://img58.imageshack.us/img58/6545/rye3zj6.jpg

FEED ME BACK :P


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Offlinerokin
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Re: 1st timer Australian [Re: rokin]
    #6415274 - 01/01/07 12:47 PM (14 years, 4 months ago)

Well, i threw away jars 2,5 and 8 cuz they looked too nasty.
Now i only wish the healthy jars wont get infected too.


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Offlinerokin
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Re: 1st timer Australian [Re: rokin]
    #6422102 - 01/03/07 06:42 PM (14 years, 4 months ago)



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Offlinegoodolgoogy
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Re: 1st timer Australian [Re: rokin]
    #6422112 - 01/03/07 06:44 PM (14 years, 4 months ago)

Quote:

rokin said:
Well, i threw away jars 2,5 and 8 cuz they looked too nasty.
Now i only wish the healthy jars wont get infected too.




I took a look at the pics.. they didn't look that bad I would have isolated and waited a few days.


--------------------
RogerRabbit said:
Don't assault your mycelium with peroxide.  That's like throwing acid in your girlfriends face just to see if it sizzles.


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Offlinerokin
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Re: 1st timer Australian [Re: goodolgoogy]
    #6425507 - 01/04/07 08:06 PM (14 years, 4 months ago)

hey
well ive got an important question.
its hard to get casing material like peat and coco coir where i live.
all i can get is those jiffy peat pellets and that those coco FIBERS.
atleast vermiculite is no problem to get
well my substrate will be ready soon and maybe you guys can help me decide how to case. do u think i can use those peat peellets instead of peat?


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Offlinerokin
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Re: 1st timer Australian [Re: rokin]
    #6425523 - 01/04/07 08:10 PM (14 years, 4 months ago)

oh... yeah
i forgot to mention i also have organic compost combined with peat that i got. could that be of any use?


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Offlinegoodolgoogy
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Re: 1st timer Australian [Re: rokin]
    #6425641 - 01/04/07 08:46 PM (14 years, 4 months ago)

I've never even attempted casings so I might be overstepping my bounds of authority on the subject but I know that horse poo and peat are often used as a casing so to my knowledge... that would probably work.


--------------------
RogerRabbit said:
Don't assault your mycelium with peroxide.  That's like throwing acid in your girlfriends face just to see if it sizzles.


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