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InvisibleSumGuy
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Storing a mushroom that you want to clone
    #639842 - 05/22/02 03:18 AM (14 years, 10 months ago)

Question is this ... My friend is fruiting some PESA's and there's this HUGE mofo' that he wants to clone on agar, only problem is he wont have a chance to pour any dishes until monday but tomorrow is when it will be picked ... can a piece of it be cut and stored in a ziplock in the fridge for 4 days ... and then cloned?


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OfflineAnnoA
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Re: Storing a mushroom that you want to clone [Re: SumGuy]
    #639846 - 05/22/02 03:32 AM (14 years, 10 months ago)

>can a piece of it be cut and stored in a ziplock in the fridge for 4 days

Better put a mushroom in a paper bag and in the fridge, and then clone it when you have agar.
OR cut a small piece of the inner tissue and store in (slightly peroxidated?) sterile water.


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InvisibleSumGuy
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Re: Storing a mushroom that you want to clone [Re: Anno]
    #639856 - 05/22/02 04:04 AM (14 years, 10 months ago)

good plan anno.. thanks a bunch


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Offlineavarrin
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Re: Storing a mushroom that you want to clone [Re: Anno]
    #639867 - 05/22/02 04:34 AM (14 years, 10 months ago)

Once the agar is fully colonized... do you just cut a peice off and place it into your jar of grains that you want to spawn?

~ava


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InvisibleMushroom_Madness
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Re: Storing a mushroom that you want to clone [Re: SumGuy]
    #639907 - 05/22/02 05:58 AM (14 years, 10 months ago)

I would suggest cutting out a piece of the specimens tissue, and then storing it into a ***NO SPAM HERE OR I'LL BAN YOU*** by dropping a piece through the Laboratory access port, closing it, and then refrigerate it. It is an air tight seal with sterile conditions. (A mixture of h2o2 w/water will be a good medium to place it in.)


Edited by CLuB99 (06/03/02 02:12 AM)


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InvisibleHippie3
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Re: Storing a mushroom that you want to clone [Re: SumGuy]
    #641166 - 05/23/02 03:33 AM (14 years, 10 months ago)

your best bet is to leave it attached to the cake, and put then entire cake in the fridge, it will stay alive for weeks.


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InvisibleSumGuy
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Re: Storing a mushroom that you want to clone [Re: Hippie3]
    #642049 - 05/23/02 05:03 PM (14 years, 10 months ago)

My friend said it was a wheat straw casing ... that'd be hard to do


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OfflineMeneerCactus
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Re: Storing a mushroom that you want to clone [Re: Mushroom_Madness]
    #642589 - 05/24/02 02:12 AM (14 years, 10 months ago)

I would suggest cutting out a piece of the specimens tissue, and then storing it into a Dynamic Spore-a-Nator


How much advertisement can you make in one post?
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OfflineMAIA
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Re: Storing a mushroom that you want to clone [Re: SumGuy]
    #642842 - 05/24/02 08:12 AM (14 years, 10 months ago)

Spore-a-what ?
Use a slant tube with perioxidated water and put it inside the fridge.

Peace,
MAIA


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InvisibleSumGuy
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Re: Storing a mushroom that you want to clone [Re: MAIA]
    #643024 - 05/24/02 10:08 AM (14 years, 10 months ago)

my friend said they just opened a bottle of spring water used like 3/4's of it to wet perlite in a terarrium and then threw in a few mL's of peroxide into the remaining water and threw a few pieces of the mushroom in it then put the bottle in the fridge... should work ok... I guess he'll see... that advertisement is funny too :P The person probably doesn't need a dynamic thingie... They're from the netherlands anyway ordering that would be too much work :P


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-SumGuy :cool:


Edited by SumGuy (05/24/02 10:10 AM)


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InvisibleDreaMaTrix
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Re: Storing a mushroom that you want to clone [Re: SumGuy]
    #643031 - 05/24/02 10:14 AM (14 years, 10 months ago)

Let us know how it goes please!!!!
My friend tried to store a piece in peroxidated agar, only to have the sample turn blue, it didn't grow on agar Maybe too much h2o2.

Good luck


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InvisibleBillyGrass
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Re: Storing a mushroom that you want to clone [Re: SumGuy]
    #645406 - 05/26/02 03:09 AM (14 years, 10 months ago)

How are fresh mushrooms at the grocery stored? Refrigerate, and make sure there are holes in whatever you choose to store it in.


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Offlinebassplayer74
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Re: Storing a mushroom that you want to clone [Re: Anno]
    #645558 - 05/26/02 07:24 AM (14 years, 10 months ago)

Hey...Is it also possible to cut a small piece of mushroom tissue and drop it directly into your sterilized jar of grain? This would seem easier. Is there any reason why this wouldnt work as well as using agar? Thanks ...


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Offlinebassplayer74
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Re: Storing a mushroom that you want to clone [Re: bassplayer74]
    #647284 - 05/27/02 08:08 AM (14 years, 10 months ago)

I am going to try this unless someone tells me its a terrible idea...bump


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Invisiblemycofile
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Re: Storing a mushroom that you want to clone [Re: bassplayer74]
    #653209 - 05/30/02 11:51 AM (14 years, 10 months ago)

Grain can be innoculated directly with fruit samples. I'd add peroxide to the grain post cooking and cooling for sure (10-ish cc's per quart). Expect a lag period (couple days) for the tissue to switch gears from tissue mode to mycelium mode.

Fruit samples can also be stored in sterile distilled water in the fridge for several weeks (IME at least 3 weeks). I'd also add some peroxide to this water (allow for ventilation of gas).

I've also stored a fruit in the fridge in a paper bag for a few days, but like the sterilty of distilled water and peroxide in a jar better, and also think it keeps longer. Then again, I never tried it dry and in a bag for more than a few days.

Best of luck.


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Invisiblepsyphon
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Re: Storing a mushroom that you want to clone [Re: mycofile]
    #653344 - 05/30/02 12:49 PM (14 years, 10 months ago)

Would it also work to put the inner stem piece into some H2O2 and let it react for a little bit and then drop it in the grain jar? Would it still be necessary to add H2O2 to the jar?

Thanks


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I wish you all ceaselessly flowing moments of happiness.


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Offlinebassplayer74
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Re: Storing a mushroom that you want to clone [Re: mycofile]
    #653456 - 05/30/02 01:54 PM (14 years, 10 months ago)

Thanks Mycofile...I always trust and value you posts.


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Offlinebassplayer74
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Re: Storing a mushroom that you want to clone [Re: psyphon]
    #653465 - 05/30/02 01:56 PM (14 years, 10 months ago)

Good question psyphon...That's pretty much what I was gonna try. Seems almost too easy though.


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Invisiblepsyphon
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Re: Storing a mushroom that you want to clone [Re: bassplayer74]
    #659447 - 06/02/02 07:39 PM (14 years, 10 months ago)

Thanks, I hope it works for you. It does seem too easy but it also seems like it should work and it'd be great if it did! As soon as my friend has some suitable fruits I'll have her give it a try, and I'll post info on her results.



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"The real voyage of discovery consists not in seeking new landscapes but in having new eyes."
- Marcel Proust

I wish you all ceaselessly flowing moments of happiness.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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