Home | Community | Message Board

Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Brown Rice Flour, pH Test Strips, Vermiculite

Jump to first unread post. Pages: 1

Registered: 06/02/01
Posts: 117
Last seen: 14 years, 6 months
Excellent Spore Rehydration Media
    #639506 - 05/21/02 06:34 PM (15 years, 6 months ago)

Just to pass along the results of a pseudo-test.

Two test tubes of L-broth (10 mL broth) were each innoculated with a large innoculation loop worth of spores that were sitting around for approx 8 months. Tubes were placed at 72-75 F; 48 hrs later cloudy mycellial network was visible surrounding/intermixing the spores.

L-broth: 1% peptone, 0.5% yeast nutrient, 0.5% NaCl, pH = 7.2 (contact your local science supplier)

Experiment was done because no growth was seen from a mycellium xfer to honey water (pH=?) done over a month prior.

Post Extras: Print Post  Remind Me! Notify Moderator
Registered: 02/24/02
Posts: 74
Last seen: 13 years, 6 months
Re: Excellent Spore Rehydration Media [Re: homebrew]
    #639523 - 05/21/02 06:55 PM (15 years, 6 months ago)

I usually have very good results with honey water tec but I have seen others have problems. What was ratio of honey to water...I wonder if that may be reason for so many failures. I just use about a teaspoon to a cup of water then I microwave for 5 mins.
I did some 1/2 pint jars aout 2 weeks ago with 1/3 cup chunky BRF, 2/3 cup vermiculite, and 1/3 cup of 4 day old honey water culture. mixed the jars up dry and did em in 300F oven for 1 hr let em cool and just dumped culture in, closed up and shook like hell. They colonized in 5 days at 80F. No contams. I'm not suggesting this as a way of growing jars but it worked.

I'm never SURE of anything

Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Brown Rice Flour, pH Test Strips, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* spore rehydration iggy 746 3 01/07/04 07:39 PM
by iggy
* spore rehydration Anonymous 565 3 07/22/03 12:45 PM
by Anonymous
* spore rehydration antimatt3r 472 1 04/03/07 09:42 AM
by agar
* spore rehydration in syringe crazychild5 527 2 10/29/04 02:19 AM
by dog
* Letting Spores Rehydrate? tex1 1,266 11 11/20/08 03:29 PM
by seven
* 12 hours for spores to "rehydrate"?! WOmbdAbblER 1,313 4 01/09/02 04:39 PM
by Champion des Champignons
* Rehydrating spores?!!! With pics tahoe 1,277 8 05/16/05 01:34 AM
* spore print -> spore syringe question baltazar 967 4 03/11/04 09:42 AM
by baltazar

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
597 topic views. 13 members, 80 guests and 20 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Everything Mushrooms
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.007 seconds on 19 queries.