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JJJJJJ
Seeker of truth
Registered: 10/12/06
Posts: 119
Last seen: 17 years, 17 days
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LC vs. Spore colonization
#6391366 - 12/20/06 05:30 PM (17 years, 3 months ago) |
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Howdy all,
Looking for advice, opinions, reassurance, with regards to some jars i have incubating right now. Interestingly enough 6 are colonized from the same LC and about 6 are from my first homemade spore syringe. They are PF cakes.
The ones inoculated with the LC turned whitish almost right away (sorta where they got wet) with real wispy looking mycelium along the edges of each inoculation point. The wispy strands spread fairly quickly at first then seemed to slow a bit. There are now the odd spot with the more familiar white ropy mycelium forming within the white area.
The ones inoculated with spores did nothing for the first few days but now show excellent growth looking very rhizomorphic ( i hope i used the word right)
Both sets of jars are from the same batch contents cooking everything.
I kind of thought it might be cobweb mold from the LC at first i don't think so now. I also thought it might have something to do with the jars being a bit dry.
So the questions this whole experience brings to mind are:
1. Can you get cobweb mold contamination from a bad LC? Does it even happen in jars?
2. Does older LC produce weaker mycelium? The one i used is about 2months in the fridge now. Jars made when it was fresh were very rhizomorphic.
3. Is everyone finished with their Christmas shopping?...just kidding.
Thanks, JJJJJJ
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creamcorn
mad scientist
Registered: 03/13/06
Posts: 2,962
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Re: LC vs. Spore colonization [Re: JJJJJJ]
#6391427 - 12/20/06 05:45 PM (17 years, 3 months ago) |
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sounds like perfectly normal growth characteristics for an LC. it does start out in wider areas, spread quickly, and look whispy and weak at first. it thickens up and gets nice and white after a few days.
an LC can carry any contamination. its all a matter of procedure. cobweb is rarity in jars but not impossible.
older LCs shouldn't produce weaker mycelium. as i learned the hard way just recently though, they can become contaminated bacteria, which apparently in certain cases can cause less healthy mycelium growth yet isn't "strong" enough to contaminate your whole jar. take a look at this thread i recently posted: http://www.shroomery.org/forums/showflat.php/Number/6365648
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: LC vs. Spore colonization [Re: JJJJJJ]
#6391431 - 12/20/06 05:46 PM (17 years, 3 months ago) |
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#1 I have never seen cobweb in jars,only on casings so I am not sure but as long as yours are nice and white now I think you are in the clear
#2 fresh LC is always better than old LC but I have used LC way more than 2 months old with no problems so once again I think you are fine
#3 yes I am finished with my Christmas shopping although I just finished yesterday
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JJJJJJ
Seeker of truth
Registered: 10/12/06
Posts: 119
Last seen: 17 years, 17 days
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Re: LC vs. Spore colonization [Re: Blutjager]
#6391482 - 12/20/06 06:02 PM (17 years, 3 months ago) |
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Thanks for the fast answers guys!
That's about what i thought but a second opinion always helps. I'd only used LC with grains except for a dozen pf+cracked corn jars that where the most rhizomorphic I've ever seen, granted that's not all that much yet.
PS. Blutjager the jars are for spawning, I've been experimenting with adding grains to PF style jars and so far it's the most reliable and easy method for me.
Thanks Again Folks. JJJJJJ
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: LC vs. Spore colonization [Re: JJJJJJ]
#6391507 - 12/20/06 06:15 PM (17 years, 3 months ago) |
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Quote:
JJJJJJ said:
PS. Blutjager the jars are for spawning, I've been experimenting with adding grains to PF style jars and so far it's the most reliable and easy method for me.
Out of curiosity isn't adding grains to pf jars defeat the point..I mean than you would still need a PC for the grains completely defeating the only reason anyone in the world should ever do pf anyway(Cause they don't have a PC)If you have a PC you would be better of making up quarts so you have that much spawn with infinitely less work
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buddhasninja
Stranger
Registered: 11/29/06
Posts: 83
Last seen: 14 years, 7 months
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Re: LC vs. Spore colonization [Re: JJJJJJ]
#6391514 - 12/20/06 06:18 PM (17 years, 3 months ago) |
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Ok, I could be wrong, but what the hell. I believe the reason that your LC inoculated jars colonized quicker than the spore inoculated jars is simply because the spores in the jars have to germinate. That takes a few days, and once germinated, then you'll start to see mycelium growth. The LC, which is basically mycelium in water, doesn't have anything to germinate, it's already done, and it just has to start feeding on your cakes and grow.
Good luck!
-------------------- Place the patty on a sesame seed bun, in accordance to the principle of balance as mentioned by the Buddha. This may be an ordinary bun, but a true ninja prefers to toast the bun lightly in order to better savor the flavor of his burger.
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JJJJJJ
Seeker of truth
Registered: 10/12/06
Posts: 119
Last seen: 17 years, 17 days
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Re: LC vs. Spore colonization [Re: buddhasninja]
#6391957 - 12/20/06 08:35 PM (17 years, 3 months ago) |
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I have a PC so i use it either way. The jar with the grains seem to colonize faster and also have added nutrients. I ialso haven't had much luck with grains, about 40% contam vs 0%. I also like the way you can control the size of the pieces when you break up a cake. I found WBS a bit small for use with straw.
I haven't given up on using straight grain, just need to break a streak of bad luck so to speak.
Buddhasninja I expected the delay, that part is normal. I was more the looks of the LC at first that had me a little scared.
Cheers, JJJJJJ
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: LC vs. Spore colonization [Re: JJJJJJ]
#6392107 - 12/20/06 09:08 PM (17 years, 3 months ago) |
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Quote:
JJJJJJ said: I have a PC so i use it either way. The jar with the grains seem to colonize faster and also have added nutrients. I ialso haven't had much luck with grains, about 40% contam vs 0%. I also like the way you can control the size of the pieces when you break up a cake. I found WBS a bit small for use with straw.
I haven't given up on using straight grain, just need to break a streak of bad luck so to speak.
Buddhasninja I expected the delay, that part is normal. I was more the looks of the LC at first that had me a little scared.
Cheers, JJJJJJ
Try rye,I never was able to get wbs to work until I already got good at rye,its allot more forgiving but the principal is the same so once you can do one you can apply what you know to the other,You will still always go back to rye though
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JJJJJJ
Seeker of truth
Registered: 10/12/06
Posts: 119
Last seen: 17 years, 17 days
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Re: LC vs. Spore colonization [Re: Blutjager]
#6392212 - 12/20/06 09:28 PM (17 years, 3 months ago) |
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I used to drink a lot of rye....leads to fights though...lol
I only tried rye once....it was too wet ended up fubar. That was my first attempt at any grain though.
G'night JJJJJJ
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Fingerfoods
Pale Blue Dot
Registered: 11/14/06
Posts: 181
Last seen: 11 years, 9 months
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Re: LC vs. Spore colonization [Re: JJJJJJ]
#6392715 - 12/21/06 12:05 AM (17 years, 3 months ago) |
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When you let an LC sit for 2 months do you periodicly have to adjitate it? Or can you just let it sit and clump together?
-------------------- http://palebluedot.ytmnd.com/ watch this shrooming and youll never think the same about the world agian.
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Oatman2000
-=Outa Space=-
Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 6 months
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Re: LC vs. Spore colonization [Re: Fingerfoods]
#6393301 - 12/21/06 09:16 AM (17 years, 3 months ago) |
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Quote:
Fingerfoods said: When you let an LC sit for 2 months do you periodicly have to adjitate it? Or can you just let it sit and clump together?
let it clump... then agitate when needed.
2months is not long for LC. 6 is when it starts to not work anymore
-------------------- Spawning to COIR My Chocolate Recipe WBS QUART SPAWN JAR PREPERATION ---------------------------- 4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: LC vs. Spore colonization [Re: Oatman2000]
#6394112 - 12/21/06 01:40 PM (17 years, 3 months ago) |
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Yes,let it get thick and healthy and than shake it up when you want to use it
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