Home | Community | Message Board


Avalon Magic Plants
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Cultivation Supplies, Injection Grain Bag   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon Agar, Laminar Flow Hood

Jump to first unread post. Pages: 1
Offlinebnjenkin
Stranger
Registered: 10/22/06
Posts: 3
Last seen: 14 years, 3 months
UV light to sterilize
    #6381609 - 12/18/06 12:47 AM (14 years, 4 months ago)

I have just built a glovebox and am looking for a way to further prevent contamination.

I had the idea to mount a UV germicidal light in it to sterilize the surfaces and the air in the box. I know the UV would kill the spores so I would wrap the syinge in foil, put everything in the box, turn the light on, wait a few seconds, then turn it off and start inoculating. Even if I didn't wrap the syringe in foil I don't think it would kill the spores b/c UV can't pass through plastic (but I could be wrong about that)

I had posted another forum about this and some one told me it was overkill and I should just build a hood. Seems odd that a $10.00 UV light is overkill but a hood is just what I needed.

would the UV thing work? Has anyone ever heard of this being done?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleBlutjager
Inhuman
Male

Registered: 06/12/06
Posts: 9,220
Re: UV light to sterilize [Re: bnjenkin]
    #6381628 - 12/18/06 12:56 AM (14 years, 4 months ago)

You don't need a UV lamp and you don't need a hood either,get a glovebox and a solution of 10-12% bleach,its all you need and will do you just fine


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: UV light to sterilize [Re: bnjenkin]
    #6381642 - 12/18/06 01:01 AM (14 years, 4 months ago)

Build a good quality still air glove box.
Make it large enough to work comfortably in.

UV is DANGEROUS, without a LOT of precautions.
Would you enjoy skin cancer :eek:


--------------------


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,200
Loc: upstate NY, USA
Last seen: 11 days, 9 hours
Trusted Cultivator
Re: UV light to sterilize [Re: agar]
    #6382594 - 12/18/06 11:46 AM (14 years, 4 months ago)

Agar is correct. Gloveboxes work by having a still air environment, not by being sterile, which they are not. A still air glovebox works as well as a laminar flow hood, just not quite as convenient.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineGrogan
Shroomieologist
 User Gallery

Registered: 05/20/03
Posts: 1,146
Last seen: 5 years, 11 months
Re: UV light to sterilize [Re: RogerRabbit]
    #6382599 - 12/18/06 11:48 AM (14 years, 4 months ago)

Screw uv.. get a flow hood if your that worried!


--------------------
"Maybe a cow occasionally ate a shroom, but it certainly wouldn't be such a potent shroom that the cow would be trippin balls. " LOL


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineYuckMan
Student
Male

Registered: 11/28/06
Posts: 69
Last seen: 4 years, 5 months
Re: UV light to sterilize [Re: Grogan]
    #6385290 - 12/19/06 05:06 AM (14 years, 4 months ago)

UV light also produces ozone gas. I don't know how long it would hang around in the box, or what kind of effects it would have on the spores, but i'd imagine it couldn't be good.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinedonkers5257
Grand Inquisitor
Registered: 12/10/06
Posts: 52
Last seen: 13 years, 7 months
Re: UV light to sterilize [Re: YuckMan]
    #6385731 - 12/19/06 11:14 AM (14 years, 4 months ago)

Back home my parents have a germicidal lamp (one parent has worked in hopsitals for ever) sitting in their basement and I considered it but decided it's applications were too limited to bother taking the unit (it's a freakin' huge 4-lamp fixture)

Is a single-bulb lamp necessary for the glovebox? No. People seem to manage just fine without it. But hey if you can pick one up cheap, why not? ($10, seriously?) You could use it for sterilizing some of your equipment and work/storage areas. Just remember that UV can't pass through opaque surfaces so only what is actually exposed to the direct UV waves gets sterilized. If it's only $10, it's probably worth installing it in an the vent (if it's possible to do it) nearest the area where you'll be incubating/fruiting, just to reduce the number of airborne contaminants.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,200
Loc: upstate NY, USA
Last seen: 11 days, 9 hours
Trusted Cultivator
Re: UV light to sterilize [Re: donkers5257]
    #6386051 - 12/19/06 12:42 PM (14 years, 4 months ago)

No. UV light causes mutations in fruits and mycelium. It causes cancer in humans. It's also worthless for sterilizing tools. UV is not a suitable choice for mycology. Trash it and follow established sterile technique.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: North Spore Cultivation Supplies, Injection Grain Bag   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon Agar, Laminar Flow Hood

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* UV sterileization 2Experimental 2,668 14 07/30/10 08:07 PM
by Doc_T
* UV Sterilization COMBO_RICO 991 10 03/06/17 07:15 PM
by mushpunx
* Question about sterility??? VIgnisFatuusI 1,199 14 07/10/03 10:58 PM
by VIgnisFatuusI
* Need advice on sterility & trading spores. The_Gnome_King 3,178 16 06/25/20 07:22 AM
by Zakkery
* sterilizing straw in oven bags Sterile 1,627 12 03/15/05 10:04 AM
by Sterile
* Every how often do i have to sterilize the misting water? Sterile 1,425 3 05/26/04 11:48 AM
by Sterile
* uv light through glass pat126 1,806 12 03/05/17 04:16 PM
by bodhisatta
* UV Germicidal Bulbs gsmith 720 4 12/15/04 10:42 AM
by gsmith

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
1,786 topic views. 15 members, 105 guests and 49 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
MRCA Tyroler Gluckspilze
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.005 seconds on 13 queries.