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cannabisg
Stranger

Registered: 03/21/06
Posts: 182
Last seen: 8 years, 9 months
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LC extraction for inoculation>?
#6370211 - 12/14/06 12:27 PM (17 years, 1 month ago) |
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This is my first time doing a LC that I purchased from one of the sponsors, question is how do i properly extract the mycelium out of the jar? I know when I inoculated it with 2ml spore there was vaccumm in the jar from PC. I have empty used syringes, just need to know the procedure. Any help appreciated.
Edited by cannabisg (12/14/06 12:28 PM)
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Abortfungi
:rootshroomsuggestion:


Registered: 05/01/06
Posts: 148
Last seen: 6 years, 6 months
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Re: LC extraction for inoculation>? [Re: cannabisg]
#6370536 - 12/14/06 01:57 PM (17 years, 1 month ago) |
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I simply poke another hole to the can if this vacuum effect happens. I use baby food jars(125ml) with metal screw caps. I like to do small batches, cause there's a smaller risk all my shit to get contaminated.
It would help to know what kinda LC are you using.
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MustardMan
Peace Frog


Registered: 10/18/05
Posts: 970
Last seen: 5 years, 5 months
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Re: LC extraction for inoculation>? [Re: Abortfungi]
#6370563 - 12/14/06 02:05 PM (17 years, 1 month ago) |
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Yea, like abort said, just take a sterile poker instrument and poke a whole in the side (as far to the side as you can, this makes it easy to extract by just poking your needle through it and tilting the jar)
I use one of these

Because they're very sharp and I've found the size whole is perfect for a syringe to fit through.
Also, for the future, I suggest you buy some RTV Silicon from an autoparts store (very durable) and apply some over the hole you've made on the side- this creates a self healing injector port 
You can then poke a larger whole in the middle of the lid and stuff with polyfill or cover with tyvek to allow gas exchange.
-------------------- Wild Psilocybe Ovoideocystidiata
 Cultivated Cubensis

Edited by MustardMan (12/14/06 02:06 PM)
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cannabisg
Stranger

Registered: 03/21/06
Posts: 182
Last seen: 8 years, 9 months
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Re: LC extraction for inoculation>? [Re: MustardMan]
#6371994 - 12/14/06 09:24 PM (17 years, 1 month ago) |
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Light malt extract/dextrose, My LC is fully colonized poking a hole with there still being a good amount of vaccumm wont that just introduce contams?
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: LC extraction for inoculation>? [Re: cannabisg]
#6372027 - 12/14/06 09:30 PM (17 years, 1 month ago) |
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Quote:
cannabisg said: Light malt extract/dextrose, My LC is fully colonized poking a hole with there still being a good amount of vaccumm wont that just introduce contams?
Do it in a freshly sterilized glovebox just to be safe
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SaturnGlass
So white andnerdy
Registered: 05/12/06
Posts: 804
Loc: The other side
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Re: LC extraction for inoculation>? [Re: Blutjager]
#6372053 - 12/14/06 09:34 PM (17 years, 1 month ago) |
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Take a plunger out of a syringe and stuff pollyfiber inside the syringe, inject that syringe into the silicon injection site. Then take the syringe that you want your LC in inject into the silicon and extract the LC
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cannabisg
Stranger

Registered: 03/21/06
Posts: 182
Last seen: 8 years, 9 months
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Re: LC extraction for inoculation>? [Re: SaturnGlass]
#6372063 - 12/14/06 09:38 PM (17 years, 1 month ago) |
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Thanks, sounds like a plan
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