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Invisibleroach6
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i need tips on pouring slants
    #6349348 - 12/08/06 11:25 PM (14 years, 5 months ago)

When you pour slants do you lay the tubes on there side and then use a funnel? Or pour them stading up then lay them down?


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Invisibleroach6
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Re: i need tips on pouring slants [Re: roach6]
    #6349439 - 12/08/06 11:59 PM (14 years, 5 months ago)

Okay i just read in TMC to pour 1/3 of the tube with agarthen plug with cotton (polyfil would be better?) and cover with foil. PC for 30 min at 15 psi. Once they are done take them out and shake.

This method is better then cooking the agar in a bottle and the pouring?


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Offlinenotagoodusername
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Re: i need tips on pouring slants [Re: roach6]
    #6349455 - 12/09/06 12:04 AM (14 years, 5 months ago)

the only problem with pc'ing agar in the jar then pouring is that you risk contam during the transfer from jar to tube. It will work either way as long as you are sure your in a contam free environment. I would be careful about shaking the tubes after pc'ing if you have a polyfill plug - don't get the plug wet. More like swirling them as opposed to SHAKING.


Edited by notagoodusername (12/09/06 12:09 AM)


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OfflineDem_Bones
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Re: i need tips on pouring slants [Re: notagoodusername]
    #6349629 - 12/09/06 01:00 AM (14 years, 5 months ago)

well there is no reson to shake it if you premixed the agar

Big time note ... do not remove the presser releaf from a PC that has agar in it, like any other liqued it will boil over big time and you will have the shit everywhere ,, you MUST let it cool one its own


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Re: i need tips on pouring slants [Re: Dem_Bones]
    #6349680 - 12/09/06 01:25 AM (14 years, 5 months ago)

good point on both


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Invisibleroach6
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Re: i need tips on pouring slants [Re: notagoodusername]
    #6349709 - 12/09/06 01:38 AM (14 years, 5 months ago)

I have a hood and i know how to pc agar. The method above was in TMC by Paul Stamets. I was asking if there is a better way to make to make them.


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OfflineRogerRabbitM
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Re: i need tips on pouring slants [Re: roach6]
    #6349783 - 12/09/06 02:09 AM (14 years, 5 months ago)

TMC is twenty years old now, and we have better ways of doing lots of things.

Fill your test tubes 1/2 full. I always insert a piece of wood, such as a medical tongue depressor into each slant. The mycelium colonizes the wood and lasts much longer under storage.

Get test tubes with screw on lids. Drill a 1/8" hole in the lid. Cut a synthetic filter disk to fit inside the test tube lid. Screw the lid down tight, and PC in a rack with the test tubes standing upright.

When removed from the PC, lay the rack over with a jar lid or something under one side so the test tubes are at an angle as they cool. Try to get the mycelium within 1/2" or so of the opening, so you don't have to reach in as far when you want to get some myceium out. You'll have to flame any part of your scalpel that penetrates into the slant, so it's easier if you don't have to reach in as far.

I use triple the usual amount of water in the PC when I make agar so that the PC cools down slower and releases pressure slower to prevent boil over. One should NEVER lift the weight or release the valve to let pressure off a PC. It ruins the product inside. Agar boils over and grains (or your pot roast) dry out.

You also shouldn't use a jar for preparing agar. Use a whiskey bottle or something similar with a long neck that is made for pouring. It also will have a smaller opening that will be less prone to allowing contaminants to enter.
RR


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