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creamcorn said:
awww come on now don't be so harsh on our beloved LCs. 

they're a trade-off, you have to be willing to accept a less than perfect success rate in exchange for not having to deal with strict sterile procedures.  there's a lot of value in that though, and its shortsighted to write them off because its less than perfect.  if you had people following the procedure you mention on a regular basis, i promise there would be just as many "why didn't it work" questions.  you only see lots of problematic posts about LCs because LCs are so popular.  im sure if folks posted every time they had a successful LC the servers would catch fire and burn to the ground.

you make perfectly valid points, but established procedures are good for experienced pros with proper equipment - so that's easy for somebody like you to say!  most around these parts have neither experience or proper equipment... things like LC aren't just a fad and its not going to go away any time soon... get used to it rather than bash it.

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Ganja420Boy said:

Does this mean it dont work or just a very low success rate?
I just did this yesterday sure hope I didnt waste my valuable spores on something so crapy

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It will more than likely work just fine,don't worry just leave it alone for a week and than go look at it 

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RogerRabbit said:
I just strongly feel new growers are doing themselves a disservice when they simply inject spores into honey water and then sit there waiting for something to happen. At the very least, inject directly to grains so you can visually inspect the process. Perhaps Agar's lids with injection ports will help those who have never used grains to get off to a good start.
RR

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RogerRabbit said:
You might want to read more than the last paragraph. I described a method to generate ten times as much LC in a shorter period of time without contamination. I'm not against liquid culture. I use it all the time. I'm simply dead against the idea of squirting a syringe into a bottle of karo/water. I insist on having at least some verification my LC is good before I inject a bunch of jars.

The analogy of a new growers greatest fear doesn't hold water. If his pf jars contaminated due to contaminated spores/syringe, it does no good to have even ten gallons of contaminated LC on hand. If they contaminated due to his sloppy procedure, he's likely to have used the same sloppy procedure making an LC.

In my posts, I always try to show a way to have the greatest possible success rate, not necessarily the cheapest, easiest or laziest method.
RR

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RogerRabbit said:
Throwing spores into honey or karo has to be the worst possible way to make a liquid culture and I'll be glad when this current fad passes and folks can get back to established techniques.  Just inoculate some grains or brf in the standard way and you'll be miles ahead.

For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores.  Upon full colonization, shake the jar to loosen each individual kernel.  Be sure to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc.  In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains.  3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out.  You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes.  Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this.

Remember, mycelium grows poorly in water unless under constant stir which oxygenates it.  With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels.
RR

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This is awesome RR,I plan on giving this a try sometime soon,Never really can have too much lc now can you   

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Blutjager said:

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RogerRabbit said:
Throwing spores into honey or karo has to be the worst possible way to make a liquid culture and I'll be glad when this current fad passes and folks can get back to established techniques.  Just inoculate some grains or brf in the standard way and you'll be miles ahead.

For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores.  Upon full colonization, shake the jar to loosen each individual kernel.  Be sure to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc.  In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains.  3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out.  You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes.  Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this.

Remember, mycelium grows poorly in water unless under constant stir which oxygenates it.  With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels.
RR

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This is awesome RR,I plan on giving this a try sometime soon,Never really can have too much lc now can you   

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RogerRabbit said:
Throwing spores into honey or karo has to be the worst possible way to make a liquid culture and I'll be glad when this current fad passes and folks can get back to established techniques. Just inoculate some grains or brf in the standard way and you'll be miles ahead.

For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores. Upon full colonization, shake the jar to loosen each individual kernel. Be sure to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc. In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains. 3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out. You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes. Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this.

Remember, mycelium grows poorly in water unless under constant stir which oxygenates it. With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels.
RR

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