Home | Community | Message Board


FreeSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars

Jump to first unread post. Pages: 1
Offlinearchnut
member

Registered: 05/14/02
Posts: 125
Loc: the east
Last seen: 14 years, 5 days
pre packaging??
    #634137 - 05/17/02 10:29 AM (14 years, 6 months ago)

my friend and i have not recieved the spores we are about to use, but we went ahead and prepared 5 jars ( PF style) and sealed them in a plastic box which we rubbed down with alchohol and lysol. the longest time they might have to sit is about 3 or 4 days. will they still be alright to sterilize and innoculate or is this risky?

thanks in advance


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineanonymoushate
member

Registered: 03/12/02
Posts: 103
Last seen: 12 years, 3 months
Re: pre packaging?? [Re: archnut]
    #634156 - 05/17/02 10:47 AM (14 years, 6 months ago)

Not a good idea at all. Leaving the substrate unsterilized is a welcome to any number of contaminants to germinate. If something takes hold, you've lost the entire jar it was in, as the possibility that the entire jar is contaminated is present. A good rule is to never prepare substrates until you have everything ready for the sterilization/innoculation stages.


--------------------
All text in the above message, expressed or implied, is fiction.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemew
member
Registered: 05/09/01
Posts: 151
Last seen: 14 years, 2 months
Re: pre packaging?? [Re: anonymoushate]
    #634228 - 05/17/02 11:24 AM (14 years, 6 months ago)

yah if it was sterilised yes you could get by my friend has spawned qts. of bs up to 5 days after sterilising them with no problems but mixing ingredients up with out sterilising is asking for an infection unless they were promptly sterilised personally i would'nt use that,chalk it up for practice till i had all my needed supplies together then i'd start with a freshly sterilised batch thats a lotta risk there .MEW


--------------------
support the FSR


Edited by mew (05/17/02 11:29 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineellisdee625
enthusiast
Registered: 03/28/02
Posts: 271
Last seen: 13 years, 11 months
Re: pre packaging?? [Re: mew]
    #634353 - 05/17/02 01:05 PM (14 years, 6 months ago)

i do it all the time. let's think... if you just fill the jars and let them sit, the nasties that could germinate will germinate. then when you pressure cook it, you kill them all. it is easier to kill germinated nasties then dormant ones. this is a good discussion. i cant wait to read the next few replies.


--------------------
Have a smurfy day!!!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineanonymoushate
member

Registered: 03/12/02
Posts: 103
Last seen: 12 years, 3 months
Re: pre packaging?? [Re: ellisdee625]
    #634639 - 05/17/02 06:25 PM (14 years, 6 months ago)

How many heat resistant bacteria are floating around in the air? Too many to name I am sure. Sterilizing a jar with substrate that has been sitting for a few days is a bit too risky for me. Sure, we all get anxious when a batch is coming up, especially our first, but allowing uninnoculated jars to sit isn't very sterile. Even if you innoculate, there may be a well developed bacterial contamination resistant to heat. Would I allow my jars to sit for a few hours? Sure, but a few days seems too neglegent.


--------------------
All text in the above message, expressed or implied, is fiction.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 25 days, 13 hours
Re: pre packaging?? [Re: ellisdee625]
    #637515 - 05/20/02 10:45 AM (14 years, 6 months ago)

> let's think... if you just fill the jars and let them sit, the nasties that could germinate will germinate. then when you pressure cook it, you kill them all

Lets continue to think:

1) Not all contamination needs to germinate. Only spore or endorspore forming microbs need this extra time to soften them up.

2) Many microbs produce toxins as they grow. Sterilization kills the microbs, but does nothing about the toxins that may have been produced.

3) Many microbs produce spores and endospores as they grow. If something happens and you or somebody else ends up opening the jar before it gets sterilized, you (or they) could be in for a nasty surprise.

4) A single bacteria can multiply into several billion organisms within hours. It is a lot easier to kill one ogramism than a trillion organisms.


My recommendations: Let prepared substrate sit at least 12 hours and no more than 24 hours before sterilization. Do not allow sterilized substrate to set more than a few days before innoculation.


--------------------
Just another spore in the wind.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* how successful are you with germinating homemade spores? sublimesubmind 968 8 07/26/04 04:30 AM
by ATWAR
* How fast can bacteria germinate? jellyfishS 1,159 7 12/10/06 04:27 PM
by agar
* Creepers... contanimation? Cambodians.. no germination? wtf... HELP! resorb 1,987 3 09/21/06 02:28 PM
by resorb
* 2 day germination smurfyshmu 679 3 08/15/06 01:07 AM
by CureCat
* Half Contam'd / Half No Germinate mycmyc 665 2 09/28/07 07:37 PM
by RogerRabbit
* Anyone have pics of trich germinating? hightimesreader 567 0 01/14/08 10:17 AM
by hightimesreader
* uninvited germination? frogsheath 807 7 03/06/02 12:44 PM
by frogsheath
* Sour smell, but no visible contamination or germination with brf Paulverizer 1,122 2 09/09/07 12:17 AM
by Seventy

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte
760 topic views. 0 members, 4 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Mushrooms.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.04 seconds spending 0.005 seconds on 14 queries.