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Omnicracker
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Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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** An Important Lesson in Sub-Strain Variation **
#6308869 - 11/28/06 08:02 PM (17 years, 4 months ago) |
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For those that still dare to ask about strain variation, lets take a look at the variation that can exist within spores from the print of the same mushroom.
On my previous grow, i prepared 4 isolates from a Brazilian print. (BZ1. BZ2, BZ3, BZ4, clever eh?) Braz4 was the only one that even made it to fruiting that grow, so i used it for most my casings.
But for science sake, i had to check out the others again... except BZ3. fuck BZ3.
These bags are from the same batch of substrate, inoculated at the same time, incubated next to each other, and fruited on the same shelf.
the third tray is Braz4.
BZ2
IS NOT A FRUITER. a cock tease of a substrain indeed. Interestingly though, it was the first to put out pins, and a hell of a pinset at that.
but it just has a hard time developing full sized fruit bodies.
Meanwhile, BZ1 developes slender fruits with oddly pale cap color. a good fruiter, not a great pinset though.
and of course, my baby, Braz4
Anywho, i found this very fascinating as this is the first time ive gotten to work on substrain isolation. check the link in my sig. if you want to see the growlog.
Edited by Omnicracker (11/28/06 08:10 PM)
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RogerRabbit
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: Omnicracker]
#6308941 - 11/28/06 08:26 PM (17 years, 4 months ago) |
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Nice pictures. I've often said you can get as much variation off a single sporeprint as you can from 'strains' that originated half a world apart from each other. What do you think caused the fruits in that one tray to abort? RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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BigSistah
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Registered: 10/17/06
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: Omnicracker]
#6309013 - 11/28/06 08:49 PM (17 years, 4 months ago) |
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Great job of showing the story. Here’s my question: When we see this kind of variation, and select for precociousness (strong early fruiting) and/or desirable shape-color-substance, will the degree of variation is subsequent generations from spore propagation decrease?
I have two substrains going – one that closely resembles the original parent, and sub descended from the same parental spore print with thick, meaty flesh and larger, lighter brown caps. Are these morphological characteristics known to be linked to changes is psilocybin content/expression, or is that a genetic wild card?
Such a curious hobby.
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Omnicracker
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Registered: 09/18/05
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Loc: Wal-Merica
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: RogerRabbit]
#6309330 - 11/28/06 10:30 PM (17 years, 4 months ago) |
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Quote:
RogerRabbit said: Nice pictures. I've often said you can get as much variation off a single sporeprint as you can from 'strains' that originated half a world apart from each other. What do you think caused the fruits in that one tray to abort? RR
it wasnt just in that one tray, i did three BZ2 trays, they all behaved the same.
POOR FRUITER
and are continuing to look bizarre on their second flush. ill post those once they get a little bigger and more distinguished.
As for your ? bigsistah, i imagine that by continually selecting for those characteristics over a long period of time would result in more homogenous spores.
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S33D
this side up
Registered: 11/13/05
Posts: 511
Loc: skylabONE
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: Omnicracker]
#6319299 - 11/29/06 07:14 PM (17 years, 3 months ago) |
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Quote:
Omnicracker said:
That truly is some bizarre fruit you got there.
-------------------- If my calculations are correct, when this baby hits eighty-eight miles per hour... you're gonna see some serious shit. ~Dr. Emmett Brown
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whatsthestorymg
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Registered: 07/23/06
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: S33D]
#6319752 - 11/29/06 09:45 PM (17 years, 3 months ago) |
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!!!! I wanna grow like that !!!! that was a solid pan of shrooms! gah! makes me drool thinking about it
-------------------- I pity the fool who don't enjoy a good cubie I pity the fool who don't enjoy a good van eyck My Unitarian Jihad Name is: Brother Cattle Prod of Loving Kindness
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Dihnekis
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Registered: 07/11/06
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: whatsthestorymg]
#6320128 - 11/30/06 12:16 AM (17 years, 3 months ago) |
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K
Edited by Dihnekis (11/10/08 02:34 PM)
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Babo911
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Registered: 09/05/05
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: S33D]
#6321173 - 11/30/06 11:15 AM (17 years, 3 months ago) |
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Quote:
S33D said:
Quote:
Omnicracker said:
That truly is some bizarre fruit you got there.
That is a great isolation, just do us all a favor and don't clone that fruit
-------------------- Ronhero on the Growery is me
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Omnicracker
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Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: Babo911]
#6321206 - 11/30/06 11:30 AM (17 years, 3 months ago) |
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glad to see this post wasnt erased. funny goings on around here at the shroomery... CIA infiltration?
Quote:
Dihnekis say: Very interesting. Annoying to learn that a lot of this stuff comes down to lucking out with a good substrain though.
thats a big part of it, but IME, multispore innoculations usually end up with the stronger of the substrains at the reins. BZ2 colonized at the same speed at Braz4, but it continued to keep colonizing until the bag was bright white! i thought that there was hope for it yet, but indeed, a tease.
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Babo911
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: Omnicracker]
#6324871 - 12/01/06 04:11 PM (17 years, 3 months ago) |
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Quote:
Omnicracker said: funny goings on around here at the shroomery... CIA infiltration?
Did I miss something?
-------------------- Ronhero on the Growery is me
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Omnicracker
Crusted Trolltivator
Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: Babo911]
#6326545 - 12/02/06 05:57 AM (17 years, 3 months ago) |
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miss something? i dont know. i guess in order to upload the shadow matrix into the shroomery, the bushreich gestapo had to put 'er down for a minute. in slip the little white men, mind them, their minds slip from rhythm to processed jizzum in an instant. avoid the contamination, be risen.
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Babo911
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: Omnicracker]
#6326559 - 12/02/06 06:08 AM (17 years, 3 months ago) |
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Quote:
Omnicracker said: the bushreich gestapo had to put 'er down for a minute.
Haha, on that note have you ever typed failure into google and looked at the first result?
-------------------- Ronhero on the Growery is me
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royer
±±±±±±±±±±
Registered: 05/15/06
Posts: 4,801
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: Babo911]
#6326590 - 12/02/06 06:55 AM (17 years, 3 months ago) |
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babo i herd the same thing you just said on the morning show / subastin in the morning or something like that on 106.9
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
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Omnicracker
Crusted Trolltivator
Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: ** An Important Lesson in Sub-Strain Variation ** [Re: royer]
#6327227 - 12/02/06 12:59 PM (17 years, 3 months ago) |
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thats off topic. http://www.whitehouse.gov/president/ but funny too.
wel i wanted to show you pictures of the second flush, but i seem to have lost my camera. snap.
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