the syringe dilemma - Mushroom Cultivation

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esin
cheesefondue

Registered: 11/21/01
Posts: 1,275
Loc: Lysergia
Last seen: 15 years, 7 months

the syringe dilemma

#630042 - 05/14/02 06:43 AM (23 years, 2 months ago)

After a contamination disaster i had which left me out of spores i got a print from someone. This print was really small and light (at first i didn't even see it) and i decided i would make only one syringe from it.
The syringe is completely clear. There isn't even one spore cluster visible inside the syringe - looks like just plain water.
One syringe usually gets me 4 qt jars of grain inoculated. Now i'm afraid of inoculating that many jars as i don't know if the amount of spores is enough to get them going.
I'm thinking of using the syringe to inoculate only one jar. That way, if i can get it to fully colonize i can use it to inoculate other jars and get some spore prints. On the other hand i'm afraid of doing this because if that jar gets contaminated i lose everything.
What would you do in this situation?

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Alpheratus
member

Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 18 years, 7 months

Re: the syringe dilemma

[Re: esin]
#630275 - 05/14/02 09:30 AM (23 years, 2 months ago)

i personaly would do 2 jars...and one honey water jar, or an agar dish. just my thoughts....
good luck, just take it slow and steady with every last percaution to guarentee no contams....
slow and steady wins the race.

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blasted holes into night until she bled sunshine