Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineBooming
n00b psybot

Registered: 10/05/06
Posts: 71
Last seen: 17 years, 10 months
flaming syringe
    #6299615 - 11/19/06 03:30 PM (18 years, 2 months ago)

is it really necessary to flame the syringe with a lighter 30 seconds? that seems a bit extreme to me... i alcohol swab the syringe as well after i use the lighter, therefore it seems a bit crazy to do it for 30 seconds?

is it really necessary? or could i do it for less time?

Extras: Filter Print Post Top
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
Trusted Cultivator
Re: flaming syringe [Re: Booming]
    #6299622 - 11/19/06 03:33 PM (18 years, 2 months ago)

Heat util the tip is cherry usually no more than ten seconds.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
Offlinethetonebone72
Hunter -Gatherer
 User Gallery

Registered: 03/11/06
Posts: 1,125
Loc: Oregon
Last seen: 6 years, 1 month
Re: flaming syringe [Re: hyphae]
    #6299632 - 11/19/06 03:38 PM (18 years, 2 months ago)

I inoculated 10-12 jars with the same syringe over several weeks and had no contamination using alcohol swab (paper towel) only. I venture to say I was lucky but if you are very careful, apparently you do not need to flame the syringe.


--------------------
Hunt On, Good Fellow


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: flaming syringe [Re: thetonebone72]
    #6300874 - 11/19/06 10:10 PM (18 years, 2 months ago)

Alcohol doesn't clean inside the needle. Flame until red hot, then use.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: flaming syringe [Re: RogerRabbit]
    #6300916 - 11/19/06 10:24 PM (18 years, 2 months ago)

Unless I just opened a sterile needle I always flame it until its red and than either wipe it with an alcohol or just drip a drop or two of solution to cool it before injecting

Extras: Filter Print Post Top
OfflineCepheus
Balance
Male User Gallery

Folding@home Statistics
Registered: 04/19/06
Posts: 8,266
Loc: the space between reality...
Last seen: 9 months, 14 days
Re: flaming syringe [Re: Blutjager]
    #6301347 - 11/20/06 01:03 AM (18 years, 2 months ago)

Yah I just use a sterile needle.

The only time I've had contams is when I've flamed it.


--------------------
"I only ever hope to reach equilibrium, in Nature's matrix, in line with the meridian" ~ Jehst

:sun: "...and I know that I have to keep breathing, as tomorrow the sun will rise, who knows what the tide will bring?" :sun:

Free Spore Ring Europe
Send any spare spore prints you might have and help the distribution :grin:

Open Source. Freedom.  GNU/Linux

Addicting is not a word.

Extras: Filter Print Post Top
OfflineBooming
n00b psybot

Registered: 10/05/06
Posts: 71
Last seen: 17 years, 10 months
Re: flaming syringe [Re: Cepheus]
    #6301823 - 11/20/06 06:19 AM (18 years, 1 month ago)

how often should i do this during the course of innoculating jars? should it be every couple of jars?

Extras: Filter Print Post Top
Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 11 years, 10 months
Re: flaming syringe [Re: RogerRabbit]
    #6301827 - 11/20/06 06:22 AM (18 years, 1 month ago)

Quote:

RogerRabbit said:
Alcohol doesn't clean inside the needle.  Flame until red hot, then use.
RR




:thumbup: :thumbup: :thumbup:

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: flaming syringe [Re: tonyperez420]
    #6301853 - 11/20/06 06:45 AM (18 years, 1 month ago)

If you flame between each jar, you eliminate the possibility of cross contamination.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 11 years, 10 months
Re: flaming syringe [Re: RogerRabbit]
    #6301992 - 11/20/06 08:22 AM (18 years, 1 month ago)

Quote:

RogerRabbit said:
If you flame between each jar, you eliminate the possibility of cross contamination.
RR



:thumbup: :thumbup: :thumbup:

I use rubbing alcohol and then flame :wink:

Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
OG Cultivator
Re: flaming syringe [Re: Booming]
    #6302147 - 11/20/06 09:44 AM (18 years, 1 month ago)



--------------------

Extras: Filter Print Post Top
OfflineBooming
n00b psybot

Registered: 10/05/06
Posts: 71
Last seen: 17 years, 10 months
Re: flaming syringe [Re: agar]
    #6302189 - 11/20/06 10:04 AM (18 years, 1 month ago)

question as far as air sanitization - i was using Oust air sanitizer and it says it kills odor causing bacteria.. does this mean the air is sanitized? also does the black residue that the flame leaves on the syringe cause contamination

Extras: Filter Print Post Top
Invisiblegema
Freedom from the Known

Registered: 10/24/04
Posts: 1,767
Loc: t(here)
Re: flaming syringe [Re: Booming]
    #6302196 - 11/20/06 10:10 AM (18 years, 1 month ago)

I never flame a syringe but use a new one everytime. If I get one from a vendor then I use an alcohol swab. All under a glove box.

Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
OG Cultivator
Re: flaming syringe [Re: Booming]
    #6302214 - 11/20/06 10:15 AM (18 years, 1 month ago)



--------------------

Extras: Filter Print Post Top
InvisibleFeanor
 User Gallery

Registered: 05/07/06
Posts: 1,546
Re: flaming syringe [Re: gema]
    #6302219 - 11/20/06 10:18 AM (18 years, 1 month ago)

I've recently only been using alchohol swabs, and I've had no contamination thus far. I've had contams from when I used a lighter..

The syringe needles that I now use have a large plastic part where they join the syringes. This isn't good because this plastic begins to melt as soon as I flame the needle.


--------------------

May Terence McKenna Live Long

The DMT Chronicles

Extras: Filter Print Post Top
InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: flaming syringe [Re: Booming]
    #6302297 - 11/20/06 10:44 AM (18 years, 1 month ago)

Quote:

Booming said:
question as far as air sanitization - i was using Oust air sanitizer and it says it kills odor causing bacteria.. does this mean the air is sanitized? also does the black residue that the flame leaves on the syringe cause contamination




I always wipe off the soot with an alcohol swab but I have read numerous times that it wont cause any harm

Extras: Filter Print Post Top
Invisiblecreamcorn
mad scientist
Male User Gallery

Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: flaming syringe [Re: Booming]
    #6302315 - 11/20/06 10:47 AM (18 years, 1 month ago)

Quote:

Booming said:
question as far as air sanitization - i was using Oust air sanitizer and it says it kills odor causing bacteria.. does this mean the air is sanitized? also does the black residue that the flame leaves on the syringe cause contamination




Yes, it means its "sanitized"... aka, somewhat cleaner than before. It by no means makes the air sterile or the working environment totally clean, and as you mention targets bacteria, its effectiveness on other forms of contaminates isn't necessarily very high. In other words, it might help some, but its definitely no substitute for good procedure and being careful/using common sense when it comes time to do your inoculations.

Extras: Filter Print Post Top
Offlinemycallister
Stranger
Registered: 04/14/18
Posts: 3
Last seen: 6 years, 6 months
Re: flaming syringe [Re: RogerRabbit]
    #25165679 - 04/25/18 09:28 PM (6 years, 8 months ago)

I am a noob but I am getting hung up on the flaming the syringe part. If I am flaming it red hot between each jar or petri, aren't I killing the couple of drops inside the needle? I am only injecting one or two drops per jar/petri so aren't I just injecting dead fried up spores that were in the needle part?

Extras: Filter Print Post Top
OfflineAma420
Thickems
Male User Gallery


Registered: 04/12/17
Posts: 1,159
Last seen: 3 years, 2 months
Re: flaming syringe [Re: mycallister]
    #25165719 - 04/25/18 09:50 PM (6 years, 8 months ago)

Squirt a few drops out needle tip to rid those dead spores and to cool needle before inoculation. Those few drops you are not losing that much spores


--------------------

Edited by Ama420 (04/25/18 09:51 PM)

Extras: Filter Print Post Top
OfflineAma420
Thickems
Male User Gallery


Registered: 04/12/17
Posts: 1,159
Last seen: 3 years, 2 months
Re: flaming syringe [Re: Ama420]
    #25165723 - 04/25/18 09:52 PM (6 years, 8 months ago)

Never wipe with alcohol after flame, flame sterilizes, alcohol sanitizes... just an fyi


--------------------

Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,438
Trusted Cultivator
Re: flaming syringe [Re: mycallister]
    #25165844 - 04/25/18 11:21 PM (6 years, 8 months ago)

Quote:

mycallister said:
I am a noob but I am getting hung up on the flaming the syringe part. If I am flaming it red hot between each jar or petri, aren't I killing the couple of drops inside the needle? I am only injecting one or two drops per jar/petri so aren't I just injecting dead fried up spores that were in the needle part?




Yeah you're killing those few drops in the needle but who cares? :shrug:

You're effectively sterilizing the needle and everything inside so why does it matter if it gets injected with the healthy stuff?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Extras: Filter Print Post Top
InvisibleMrRabbit-HMR
Professional Duck Washer


Registered: 08/20/17
Posts: 262
Loc: UK Flag
Re: flaming syringe [Re: elasticaltiger]
    #25165982 - 04/26/18 02:12 AM (6 years, 8 months ago)

I always squirt a little bit of solution from the syringe to cool the needle after flame steralizing. Its quicker to cool than letting it cool via air alone.

Also, it could melt any injection ports if not allowed to cool properly and if that's what people are using.

I used to use an alcohol lamp, but it took a while to get my needles and scalpel red hot, I then used a propane gas torch, but was top heavy and awkward. I now use a portable propane bunsen burner which is perfect.

Extras: Filter Print Post Top
InvisibleZiran
The Hero of Time
I'm a teapot User Gallery


Folding@home Statistics
Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
Re: flaming syringe [Re: elasticaltiger]
    #25165984 - 04/26/18 02:17 AM (6 years, 8 months ago)

Quote:

elasticaltiger said:
Quote:

mycallister said:
I am a noob but I am getting hung up on the flaming the syringe part. If I am flaming it red hot between each jar or petri, aren't I killing the couple of drops inside the needle? I am only injecting one or two drops per jar/petri so aren't I just injecting dead fried up spores that were in the needle part?




Yeah you're killing those few drops in the needle but who cares? :shrug:

You're effectively sterilizing the needle and everything inside so why does it matter if it gets injected with the healthy stuff?




if he's that worried, the first few drops go on the SAB floor, and then the next drop goes onto a plate. :super:

also, super :oldthread:


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* Flaming needle inbetween injections..... PROBLEM ganjagoa 1,887 10 12/23/02 06:16 AM
by Alien
* Flaming Spore Syring Needle Question... FreshCaps 5,853 5 08/10/05 06:41 PM
by agar
* Self healing inject points for jars Peterthinks 987 1 11/21/04 01:51 PM
by aussieone
* Getting ready to make my first spore syring fromthemoon 8,292 10 06/29/07 08:49 PM
by wikedferret
* 2 questions, 1 on ratio of contamination and the other is the need to flame a brand new needle? ICU812 825 5 08/10/05 10:49 AM
by Pinx
* Newbie about to dive in head first - amount of spore syring CCs into 4 lb grow bag?
( 1 2 all )
ChrisHippyLoser 15,187 25 06/02/16 05:47 PM
by OldSawbonesHisself
* Injecting water? RedeyedElf 1,052 2 04/11/02 12:25 AM
by sixpac440
* The whole flaming the needle..
( 1 2 all )
Cray 4,273 26 03/02/05 04:02 PM
by XTCollection

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,424 topic views. 12 members, 86 guests and 18 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.005 seconds on 12 queries.