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Invisibleelasticaltiger
Like Tigers in Coitus
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Re: flaming syringe [Re: mycallister]
    #25165844 - 04/25/18 11:21 PM (6 years, 8 months ago)

Quote:

mycallister said:
I am a noob but I am getting hung up on the flaming the syringe part. If I am flaming it red hot between each jar or petri, aren't I killing the couple of drops inside the needle? I am only injecting one or two drops per jar/petri so aren't I just injecting dead fried up spores that were in the needle part?




Yeah you're killing those few drops in the needle but who cares? :shrug:

You're effectively sterilizing the needle and everything inside so why does it matter if it gets injected with the healthy stuff?


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InvisibleMrRabbit-HMR
Professional Duck Washer


Registered: 08/20/17
Posts: 262
Loc: UK Flag
Re: flaming syringe [Re: elasticaltiger]
    #25165982 - 04/26/18 02:12 AM (6 years, 8 months ago)

I always squirt a little bit of solution from the syringe to cool the needle after flame steralizing. Its quicker to cool than letting it cool via air alone.

Also, it could melt any injection ports if not allowed to cool properly and if that's what people are using.

I used to use an alcohol lamp, but it took a while to get my needles and scalpel red hot, I then used a propane gas torch, but was top heavy and awkward. I now use a portable propane bunsen burner which is perfect.

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InvisibleZiran
The Hero of Time
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Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
Re: flaming syringe [Re: elasticaltiger]
    #25165984 - 04/26/18 02:17 AM (6 years, 8 months ago)

Quote:

elasticaltiger said:
Quote:

mycallister said:
I am a noob but I am getting hung up on the flaming the syringe part. If I am flaming it red hot between each jar or petri, aren't I killing the couple of drops inside the needle? I am only injecting one or two drops per jar/petri so aren't I just injecting dead fried up spores that were in the needle part?




Yeah you're killing those few drops in the needle but who cares? :shrug:

You're effectively sterilizing the needle and everything inside so why does it matter if it gets injected with the healthy stuff?




if he's that worried, the first few drops go on the SAB floor, and then the next drop goes onto a plate. :super:

also, super :oldthread:


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