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 matt66
member
Registered: 03/17/02
Posts: 126
Loc: Connecticut
Last seen: 15 years, 4 months
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 sinking spores 
#627483 - 05/12/02 08:09 AM (18 years, 9 months ago) |
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I got what I think is a good print from Ralphster. It was straight up black, and I shook some off (just a litte relative to what was in the aluminum foil) into a few squirts of water in a sterilized shot glass. The black stuff turned a bit more purple upon hitting the water, floated on top for a while, then everything seemed to sink to the bottom. I tried sucking up some of the spores, but when I shook the syringe up, I really couldn't see anything floating around before squirting a bit into the substrate jars (I saw the drops run down the side). Anyway, that was 3 days ago, I put the jars away and was wondering how long I should wait for the white growth up the sides before trying my hand at inculating again. Any advice on how much spore clumpage to shake off per ml of water would be great. Also, 10cc? What are the coversions to ml?
much love,
matt
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Truth is the daughter of time, time doesn't exist...
Truth cannot exist within the human convention of time- The Goat Hath Spoken
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Sterile
mushroom lover
Registered: 03/16/01
Posts: 2,526
Loc: under the Amanita
Last seen: 3 months, 12 days
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 Re: sinking spores [Re: matt66]
#627511 - 05/12/02 08:34 AM (18 years, 9 months ago) |
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Very few spores are needed to make a spore syringe.
If the print is dark and medimun sized, 1/15 - 1/20 will be enough for a syringe.
Usually people use a lot more, since it may speed up things a little bit, but i think it doesnt worth the spores...
Too many spores in a syringe can be a problem too i think.
A good thing is to srap off some spores in your shotglass, and THEN let a few drops of water fall on them. Mix well and then squirt another 5cc or so in the shotglass, mix again, and fill the syringe. This method has only been a success for mycologists around the globe 
Dont forget to shake the syringe before every inoculation to distribute the spores evenly.
Shroom on!
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The Source Of The Force
Is The Power Of The Mind
"if you don't like what you're doing, you can always pick up your needle and move to another groove." - timothy leary"
Anno: "-I can do anything with those clouds!"
Annos Tek
Edited by Sterile (05/12/02 08:36 AM)
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ChromeCrow
one ancient mutha
Registered: 02/21/02
Posts: 1,887
Loc: Hoosier HELL
Last seen: 6 months, 15 days
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 Re: sinking spores [Re: matt66]
#627715 - 05/12/02 12:22 PM (18 years, 9 months ago) |
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cc and ml are the same thing 10cc syringe=10ml syringe
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ISO: Orissa, Malabar, z strain
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