|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
FreeSporePrints

Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 14 days, 10 hours
|
UV Lamp sterilization?
#6267892 - 11/10/06 04:42 AM (17 years, 6 months ago) |
|
|
Hi there, i was wondering wheter is possible to sterilize tools as syringes, blades, tinfoil or paper with an UV lamp.
- Theorically after a bath in water + 10% bleach of 12 hours can you sterilize the used plastic petri and do not have contaminations?
- In which raw material should be the box to stop the ultraviolet rays?
- Plastic, 100% opacity, works?
- How much time is needed to sterilize something?
Thank you! Fabio
|
shirley knott
not my real name

Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 4 months
|
|
i doubt it, that'd be too easy. the dark side of the moon must be out of radio contact, or something like that.
-------------------- buh
|
minesstudent
Who knows?


Registered: 12/12/05
Posts: 400
Last seen: 11 years, 4 months
|
|
It should work for surface sterilization. They use it in hospital to sterilize equipment.
http://en.wikipedia.org/wiki/Ultraviolet_Germicidal_Irradiation
-------------------- "The universe is the way it is because if it wasn't we wouldn't be here to talk about it"
|
OldSpice
Geritol Breath...


Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
|
|
Damn Shirley quit being such a bitch It would work damn good on tools before you used them The only problem with UV`s is Retina Damage and burned hands Ive used UV light for sterilization for a long time now
 I made this one outta scraps
-------------------- So hard to be ....WDWGFH? Texas is humongus compared to France Our Gair, who art in Texas, Paw Paw be thy Name.... My friends are thirsty
You never see a motorcycle parked outside a Psychiatrist office
|
shirley knott
not my real name

Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 4 months
|
Re: UV Lamp sterilization? [Re: OldSpice]
#6270159 - 11/10/06 08:13 PM (17 years, 6 months ago) |
|
|
i love bein a shitbag. get over it, biatch 
nice tube thingies, btw. they look a bit like tits, which is good 
rool: tits
-------------------- buh
|
OldSpice
Geritol Breath...


Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
|
|
Happy birthday woman
-------------------- So hard to be ....WDWGFH? Texas is humongus compared to France Our Gair, who art in Texas, Paw Paw be thy Name.... My friends are thirsty
You never see a motorcycle parked outside a Psychiatrist office
|
FreeSporePrints

Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 14 days, 10 hours
|
Re: UV Lamp sterilization? [Re: OldSpice]
#6271196 - 11/11/06 05:56 AM (17 years, 6 months ago) |
|
|
It is possible to sterilize plastic or glass petris?
Do you have plans to build a UV box? any infos?
thank you.
Fabio
|
Mycologizt
Stranger
Registered: 11/11/06
Posts: 2
Last seen: 17 years, 2 months
|
|
Yep, use an autoclave for glass. Use gas or liquid phenol (carbolic acid) for the plastic. Best bet is to use new plastic each time. UV does nothing but damage proteins. It is not the best method for sterilization, but it will work great for maintaining a sterile storage area. Idophore is another good sterilizer. Brewers use it all the time to eradicate undesired fungi and bacteria from their materials.
As for the uv box...just build or buy an enclosed cabinet, mount a uv bulb in the top, make sure all of your items receive adequate exposure. Use a regular bulb to check for exposure and for light that may escape the cabinet. Remember that uv will cause damage to your cells. Do not expose yourself to it.
|
OldSpice
Geritol Breath...


Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
|
|
That sterilizer is for my aquarium Ive never used a UV for anything myco related
-------------------- So hard to be ....WDWGFH? Texas is humongus compared to France Our Gair, who art in Texas, Paw Paw be thy Name.... My friends are thirsty
You never see a motorcycle parked outside a Psychiatrist office
|
FreeSporePrints

Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 14 days, 10 hours
|
Re: UV Lamp sterilization? [Re: OldSpice]
#6274178 - 11/12/06 02:19 AM (17 years, 6 months ago) |
|
|
i mean to sterilize empty petri then fill them with pressure cooked media.
If the box is entire closed UV shouldn't reach my cells.
More info are appreciated!
Fabio
|
fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
|
Re: UV Lamp sterilization? [Re: Mycologizt]
#6276451 - 11/12/06 06:28 PM (17 years, 6 months ago) |
|
|
> UV does nothing but damage proteins.
UV sterilization works by damaging DNA. The T's (thymine) absorb UV radiation with a wavelength of 254nm. There are many DNA repair mechanisms, but a good dose of UV will overcome them.
UV is great for sterilizing surfaces or the air. It doesn't penetrate plastic or (non quartz) glass though, so it will only work if you can directly expose all the surfaces.
-FF
|
Mycologizt
Stranger
Registered: 11/11/06
Posts: 2
Last seen: 17 years, 2 months
|
Re: UV Lamp sterilization? [Re: fastfred]
#6276820 - 11/12/06 08:22 PM (17 years, 6 months ago) |
|
|
O.K., more specificly creating thymine dimers and thus sequencing and coding errors, mutations.....
|
ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
|
|
Quote:
FreeSporePrints said: i mean to sterilize empty petri then fill them with pressure cooked media.
If the box is entire closed UV shouldn't reach my cells.
More info are appreciated!
Fabio
forget about it, wont work out
--------------------
MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
|
elduque
Major LeagueInfidel

Registered: 09/29/06
Posts: 246
Loc: Eastern AL.
Last seen: 17 years, 4 months
|
Re: UV Lamp sterilization? [Re: ohmatic]
#6277801 - 11/13/06 01:11 AM (17 years, 6 months ago) |
|
|
i just posted about UV in the cultivation forum...someone was asking about using a tanning bed to sterilize jars ...(i know!!!) anyway here is what i posted back to him. has some relevance here. you just cant go buy a UV lamp and hope for the best, you have to get a certain lamp for killing germs.
the reason for PC'ing jars at 15 psi is just like davesj1 said so the steam can penetrate deep into the substrate. UV light as it does kill contams, would only do so on the surface area of the jar. IMHO the benefit of it is not worth the time. if you want to do an experiment with a jar that you dont plan to inoculate, i think you would see contams springing forth straight away. i could be wrong, although i am pretty sure in theory it would not work.
and i will explain further why i think it will not just so you dont think i am just some nay sayer 
not all UV light kills germs, only UV-C Output of the ultraviolet spectrum at the critical 253.7 wavelength (254 nM) will kill the contaminants. "UV-C" takes in a very broad spectrum of wavelengths. And most wavelengths in the ultraviolet-c spectrum dont do much to either purify the air or kill germs. other wavelengths produce ozone. Only an extremely narrow band in the UV-C spectrum is actually useful to purify the air and kill viruses, bacteria, etc.
Tanning beds and sun lamps generally emit 93% to 99% UV-A radiation increasing the benefits of a tan. but doing nothing for the bacteria. if sun lamps did kill all bacteria, it would probably be very harmful to be exposed to for any length of time. IMO
so i dont think you would see any good use of a tanning bed, i hope this clears a few things up for you. really you cant get much better than a steam sterilizer (PC) for what you need.
-------------------- We have a little war going on here. I just heard an ambulance go around the corner. what will it be tonight? the son who kicked his mother down the stairs? the father who beat his wife to death with the telephone? the baby who got forgotten by his mother because the drugs were really good today? you never know. you never know and you never get to know unless it's you, and if its you, well, you dont want to know. ~Henry Rollins "Art To Choke Hearts"
|
FreeSporePrints

Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 14 days, 10 hours
|
Re: UV Lamp sterilization? [Re: elduque]
#6277829 - 11/13/06 01:23 AM (17 years, 6 months ago) |
|
|
i'm not planning to sterilize media or substrate with UV rays!
my cousin that is a biologist says that she uses UV lamp for around a minute to sterilize tools and EMPTY petris. i don't know if you've ever seen that some fabrics sell cheap plastic petri unsterilized for this use..
And yes just the UV-C rays work..
For me is far better use UV rays with empty petris because i can my pressure cooker just of agar jars (i've unlucky got a 7.5 psi one, you can't imagine what a mess..)
Fabio
|
ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
|
|
i see, i just buy the plastic pre-sterilized petri plates, no hassle and they'r cheap.
id just buy a normal pressure cooker, 7.5psi is the half of 15psi which is required, so yours is like a pod with a lid on it
--------------------
MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: UV Lamp sterilization? [Re: ohmatic]
#6281361 - 11/14/06 02:22 AM (17 years, 6 months ago) |
|
|
A slight bit off the subject.
Years back, gnats managed to get in my positive pressure ventilated grow room. 
I had a scrap GE industrial strength UV bulb & mount from a weird old 60's sterilizing set-up laying around. I mounted that in the metal duct work in the intake side of the ventilation system.
That ended the gnat problem. 
It did not seem to kill them outright, as occasionally, I saw adults flying around. But, for sure, they did not reproduce in substrate trays, after that. I gather UV affects their ability to reproduce.
I wonder how?
--------------------
|
shirley knott
not my real name

Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 4 months
|
Re: UV Lamp sterilization? [Re: agar]
#6281542 - 11/14/06 07:08 AM (17 years, 6 months ago) |
|
|
maybe it fries their little gnatty bollocks
-------------------- buh
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
|
Quote:
shirley knott said: maybe it fries their little gnatty bollocks
UV can be separated into various ranges, with short range UV (UVC) considered “germicidal UV.”
At certain wavelengths UV is mutagenic to bacteria, viruses and other micro-organisms.
At a wavelength of 2,537 Angstroms (254 nm)[3] UV will break the molecular bonds within micro-organismal DNA, producing thymine dimers in their DNA thereby destroying them, rendering them harmless or prohibiting growth and reproduction.
I gather it does, in some way render them sterile
--------------------
|
|