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Offlinetallmonkey
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LC with myc or mushrooms
    #6267047 - 11/09/06 10:52 PM (14 years, 5 months ago)

I am harvesting currently with little mushies, I also feel that i wont get many spores or if i do theyll be contamed. I was wondering if i could take a piece of mycelium from the middle of a colonized cake that has allready fruited and plop it into the Liquid culture?
Could i use a piece of mushroom also the problem with that is it could have contams most likely does. thannks in advance.


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OfflineWronguy
Registered: 03/05/05
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Re: LC with myc or mushrooms [Re: tallmonkey]
    #6267110 - 11/09/06 11:05 PM (14 years, 5 months ago)

I personally wouldn't use a piece of substrate that has already been exposed to the elements, but some people do. You could take a piece of the mushroom from the center by splitting it in half. You should be able to get a nice sterile piece. I highly recommend you do this in front of a flow hood or inside a glove box. Open air transfers are a crap shoot even under the best circumstances.

If you could get a piece of the substrate from the middle, you might be able to get a sterile sample now that I think about it. Taking a sample from the center of the mushroom would be my preference though.


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Offlinechainsaw1298
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Re: LC with myc or mushrooms [Re: tallmonkey]
    #6267120 - 11/09/06 11:08 PM (14 years, 5 months ago)

from what I've been reading lately you could take some tissue from the center of the stem of one of your little shrooms and clone it now I'm not shure if you can toss that stright into a jar for lc but I have seen pics of petree dishes with clones growing


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OfflineWronguy
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Re: LC with myc or mushrooms [Re: chainsaw1298]
    #6267143 - 11/09/06 11:12 PM (14 years, 5 months ago)

Quote:

chainsaw1298 said:
I'm not shure if you can toss that stright into a jar for lc




I do it all the time. Works great.  :thumbup:


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Offlinechainsaw1298
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Re: LC with myc or mushrooms [Re: Wronguy]
    #6267244 - 11/09/06 11:31 PM (14 years, 5 months ago)

thats great that it works like that I'll have to start doing that for LC


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InvisibleBlutjager
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Re: LC with myc or mushrooms [Re: chainsaw1298]
    #6267362 - 11/10/06 12:01 AM (14 years, 5 months ago)



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Offlinetallmonkey
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Re: LC with myc or mushrooms [Re: Blutjager]
    #6267422 - 11/10/06 12:28 AM (14 years, 5 months ago)

thanks all great help giveing me a new hope


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InvisibleBlutjager
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Re: LC with myc or mushrooms [Re: tallmonkey]
    #6267440 - 11/10/06 12:40 AM (14 years, 5 months ago)

No problem and good luck


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Offlinehucker696
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Re: LC with myc or mushrooms [Re: Blutjager]
    #6267775 - 11/10/06 04:42 AM (14 years, 5 months ago)

I just made my first good LC using the link above,I had to try it four time before I got a good one.I suggest you make 5 or 6 jars at once.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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