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Offlinechrisscole
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agrocybe aegerita spore germination
    #6250462 - 11/05/06 05:18 PM (14 years, 11 months ago)

Hi there.

Was wanting to get a bit of help. I got some A. Aegerita spores from FSP about a month ago, and I made a spore syringe with them - filtered boiled water. I then inoculated a petri covered in Malt Extract Agar (food grade agar), and never got any growth. Was wondering if I could get some advice, does anyone know what could be wrong.

Not 100% sure about this as I have since thrown away the agar - after 3 wks incubating, but it may have been v slightly lighter coloured where the syringe liquid was on the agar and there didn't seem to be any disernable contamination on the agar (not any moulds anyway). I still have the syringe in the fridge, so may try it again sometime - when I get some new petris or tubes.

Chris


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InvisibleZen Peddler
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Registered: 06/18/01
Posts: 6,379
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Re: agrocybe aegerita spore germination [Re: chrisscole]
    #6252602 - 11/06/06 08:19 AM (14 years, 11 months ago)

I had a similar problem recently with a spore print of the same species - not sure how that happened. I can remember where i got mine from.
Thankfully ive got more on the way - this is a real goer of a species - when i grew it last time i got three good meals out of the first flush...


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OfflineOwl
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Registered: 03/20/04
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Loc: Netherlands
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Re: agrocybe aegerita spore germination [Re: chrisscole]
    #6252700 - 11/06/06 09:31 AM (14 years, 11 months ago)

Chris, how old are the spores? If they are old you might need to use more spores for inoculations because the number of viable spores decline as the spore print ages.

Try to use an agar recipe with a little bit less agar, 15 grams per l. instead of 20 gr,this makes the agar more moist and allow you to hydrate the spores on your agar plate.

More spores also results in more chance of contams in your inoculum. As soon as you see healthy mycelium transfer it away to a fresh plate.


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OfflineFreeSporePrints
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Re: agrocybe aegerita spore germination [Re: Owl]
    #6252738 - 11/06/06 10:01 AM (14 years, 11 months ago)

His sporeprints is not dated, i know it's 2 years old


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InvisibleSemilanceata
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Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: agrocybe aegerita spore germination [Re: chrisscole]
    #6256131 - 11/07/06 03:30 AM (14 years, 11 months ago)

Older spores don´t germinate as well as fresh ones. I had the same problem with some Sparassis crispa spores (4 years old) until I hydrated them for an enough long time. Now that your syringe has been sitting for a while spores might be well hydrated now and will germinate better. You can also try to inoc. a Karo jar or something.

Good luck.


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Sr_Setahongo


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