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 Lapius
Super Fighting Robot
Registered: 04/05/02
Posts: 308
Loc: Earth 25XX
Last seen: 9 years, 9 months
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 what do i do?? 
#623912 - 05/01/02 09:37 AM (21 years, 7 months ago) |
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hello everyone,
quick question...
what do i do if iput the rubber facing down... is there anyway i could fix them or is it too late to risk contam... its been 3 days since innoculation and there are no signs of growth yet, any input would help me alot thanks everyone,
-PeZ
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 WakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
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 Re: what do i do?? [Re: Lapius]
#623928 - 05/01/02 09:58 AM (21 years, 7 months ago) |
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Well, it's a little risky but, you could untape one hole on each jar. I hope you have a nice thick verm layer.
Free Spore Ring
--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)
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Lapius
Super Fighting Robot
Registered: 04/05/02
Posts: 308
Loc: Earth 25XX
Last seen: 9 years, 9 months
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well this is my set up on each jar.... filter disk- metal lid- ring- tin foil..
i have about an inch of dry verm as a protective agent, but do you think ifi removed the lid and flipped the lid to rubber side out i would get contam?
tanks for your help :")
-PeZ
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Seuss
Error: divide byzero
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 24 days, 3 hours
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 Re: what do i do?? [Re: Lapius]
#623931 - 05/01/02 10:02 AM (21 years, 7 months ago) |
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If you have already PC'd the jars, there is no reason to worry about the rubber seal being up or down. Leave it alone until the jars have colonized or you will most likely get contamination.
Edit: I am assuming you are using pftek with the nail holes or something similiar for air exchange. If not, I would take the rings off and crack the seal so the lids are loose and then put the rings back on loose as well.
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Just another spore in the wind.
Edited by Seuss (05/01/02 10:16 AM)
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Lapius
Super Fighting Robot
Registered: 04/05/02
Posts: 308
Loc: Earth 25XX
Last seen: 9 years, 9 months
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Re: what do i do?? [Re: Seuss]
#623933 - 05/01/02 10:05 AM (21 years, 7 months ago) |
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awsome thanks !!!
yeah i pressure cooked these babies for one hour and 10 min 
so i shouldent have a problem, but im assuming that it will take a bit longer, thanks everyone i defianitly apreciate all the help!
-PeZ
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WakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
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Re: what do i do?? [Re: Lapius]
#623937 - 05/01/02 10:07 AM (21 years, 7 months ago) |
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If there is a filter disk between the rubber seal and the glass,
I'd say you'll be fine. I would get rid of the foil though. It's just
to keep the water from dripping from above and seeping into
the jars while you're cooking.
Free Spore Ring
--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)
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XAZIA
glitter goddess
Registered: 08/22/01
Posts: 678
Loc: oklahoma
Last seen: 21 years, 2 months
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hi lapius!, waking up late! suess!
yeah take the foil off. you have plenty of protection with verm layer & filters. and you really don't need to pressure cook for one and a half hours. this time is for boiling in a regular pot. if you staerilize for too long you will dry your substrate or significantly change your water ratio. 30 minutes is suffiecient with 1/2 pts and a pc.
peace!
--------------------
"Emancipate yourself from mental slavery, none but ourselves can free our minds."
Bob Marley
Remember, EGOISM is the beginning, the source, and the root of EVIL!
http://www.fanaticus.com/pf-tek.htm
http://www.mycotopia.net/teks/hongus.html
Edited by XAZIA (05/01/02 10:15 AM)
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Lapius
Super Fighting Robot
Registered: 04/05/02
Posts: 308
Loc: Earth 25XX
Last seen: 9 years, 9 months
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thanks, but the filter disk is after the metal lid...
should i take he advise and remove the tinfoil inside the incubating chamber?
thanks
-PeZ
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indicaz
Lysol God
 
Registered: 04/12/02
Posts: 580
Loc: My Chair
Last seen: 14 years, 8 months
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 Re: what do i do?? [Re: Lapius]
#623965 - 05/01/02 10:43 AM (21 years, 7 months ago) |
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while on the subject of tinfoil....
I can remove the tinfoil once in incubator???
do I have to???? I handle my jars atleast once a day and kinda like the tinfoil layer on top.... I didnt tape the holes off and I do have a layer of verm... just thought the tinfoil would prevent water that builds up on the lid of the incubator from dripping onto and in jars and also kinda adds the extra level of security from contams... I have 3 dogs and am a smoker.. so Im always thinking that bacteria is everywhere.... plus ive had a pretty bad cold as of late.... im surprised my jars even colonized without contams considering all the bacteria running round my room...
l8r
indicaz
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XAZIA
glitter goddess
Registered: 08/22/01
Posts: 678
Loc: oklahoma
Last seen: 21 years, 2 months
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Re: what do i do?? [Re: Lapius]
#624082 - 05/01/02 01:33 PM (21 years, 7 months ago) |
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lapius...you are being way too paranoid about sterility! if i understand right you have a verm layer, a filter disk, a metal lid, tape over your holes, and tin foil over the whole lid. the verm layer and filter disk are MORE than sufficient. every thing after that,remove.
indicaz...you should not have a large quantity of water dripping in your incubator. remove the foil.
the build up of co2 levels is what promotes a healthy vegetative stage of growth (colonization). but it also needs a a small supply of fresh air.
indicaz...since you are using an incubator you can control the environment directly around your colonizing jars. whenever you go to check your jars, keep it breif and keep a jar oflysol right next to your incubator. before you open the lid, spray lysol in the area and let settle. before you put the lid back on spray a cloud of lysol in the are. and only check your jars after you take a shower or bath or at the very least right after washing your hands.
peace!
--------------------
"Emancipate yourself from mental slavery, none but ourselves can free our minds."
Bob Marley
Remember, EGOISM is the beginning, the source, and the root of EVIL!
http://www.fanaticus.com/pf-tek.htm
http://www.mycotopia.net/teks/hongus.html
Edited by XAZIA (05/01/02 01:37 PM)
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: what do i do?? [Re: XAZIA]
#624721 - 05/02/02 08:24 AM (21 years, 7 months ago) |
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" lapius...you are being way too paranoid about sterility! "
" indicaz...whenever you go to check your jars, keep it breif and keep a jar oflysol right next to your incubator. before you open the lid, spray lysol in the area and let settle. before you put the lid back on spray a cloud of lysol in the are. and only check your jars after you take a shower or bath or at the very least right after washing your hands. "
Sisi.....your starting to sound paranoid about sterility too.
lolzz
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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XAZIA
glitter goddess
Registered: 08/22/01
Posts: 678
Loc: oklahoma
Last seen: 21 years, 2 months
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 Re: what do i do?? [Re: Roadkill]
#624736 - 05/02/02 08:41 AM (21 years, 7 months ago) |
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hey roadkill! nah, he just said he had lots of dogs and stuff in his house. unforunately, i think i am actually getting a little too lax about sterility, last night i innoculated jars with just a alcohol soaked cotton ball and one glove on, on my nightstand.
--------------------
"Emancipate yourself from mental slavery, none but ourselves can free our minds."
Bob Marley
Remember, EGOISM is the beginning, the source, and the root of EVIL!
http://www.fanaticus.com/pf-tek.htm
http://www.mycotopia.net/teks/hongus.html
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: what do i do?? [Re: XAZIA]
#624737 - 05/02/02 08:45 AM (21 years, 7 months ago) |
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shame on you!!
lolzz
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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XAZIA
glitter goddess
Registered: 08/22/01
Posts: 678
Loc: oklahoma
Last seen: 21 years, 2 months
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 Re: what do i do?? [Re: Roadkill]
#624871 - 05/02/02 12:21 PM (21 years, 7 months ago) |
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i know...i should be punished severely!
--------------------
"Emancipate yourself from mental slavery, none but ourselves can free our minds."
Bob Marley
Remember, EGOISM is the beginning, the source, and the root of EVIL!
http://www.fanaticus.com/pf-tek.htm
http://www.mycotopia.net/teks/hongus.html
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indicaz
Lysol God
Registered: 04/12/02
Posts: 580
Loc: My Chair
Last seen: 14 years, 8 months
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Re: what do i do?? [Re: XAZIA]
#625399 - 05/03/02 01:11 AM (21 years, 7 months ago) |
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well really you cant bee too sterile...
i always wash my arms down with antibacterial soap first then lysol arms and hands then lysol area/room then lift lid more lysol check jars, lysol area and close lid.... doing all that Im still paranoid. but in the same room my dogs hang out, my computer is here and i smoke trees and ciggs all day.... sooo who knows how things will go. the chamber is nearly airtight, and in regards to where I prepare everything, basically a kitchen counter, cleaned with alcohol/peroxide spray area with lysol, its pretty draft free. I pressure cook and microwave everything. I even have added a little peroxide to the water I add to substrate im so paranoid. Dosent look like the peroxide has hurt anything, still worried about being sterile though always..
Xazia, amazingly I bet thatone gloved jar will do great and mine will rot.. hahahaha
l8z
indi
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biggwigg
member
Registered: 10/24/01
Posts: 112
Loc: PA
Last seen: 3 years, 5 months
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Re: what do i do?? [Re: indicaz]
#625423 - 05/03/02 01:42 AM (21 years, 7 months ago) |
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yeah i always use the rubber side down and i leave the foil on i never had a prob i even use the pint size jars you should be alright
--------------------
I have only been wrong one time in my life.
thats when i thought i was wrong.
If its not a rave its not a party
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